H 7700 tem system

CR is a diazo textile dye used for the qualitative analysis of cellulose and amyloid fibers as well as curli fibers produced by E. coli. It is also used in indirect identification measurement of c-di-GMP (48 (link), 49 (link)). E. coli W1688 and ΔycgF cell suspension samples were stained with 5 μL CR (25 mg/mL), which was followed by full-wavelength scanning to quantitatively analyze the amount of CR on the biofilm and further determine the amount of biofilm. TEM analysis facilitates high-resolution visualization of specific structural components, such as polysaccharide fibrils and environmental DNA (eDNA) (48 (link)). The two strains were cultured until an OD₆₀₀ value of 1 was recorded and then added to a 24-well plate containing fresh M63 medium and incubated under dark and blue light (450 nm; 1,300 lx) conditions for static culture at 30°C for 30 h. The bacteria were collected via centrifugation (12,000 × g, 2 min), resuspended, and washed with 1% PBS to prepare suspension samples. The suspension sample was dropped on a copper mesh with a support membrane and retained for 2 min; the excess liquid was absorbed from the edge of the droplet with filter paper. The copper mesh was inserted into the phosphotungstic acid dye solution and dyed for 15 s. After drying, the samples were observed under the Hitachi H-7700 TEM system.

CR is a diazo textile dye used for the qualitative analysis of cellulose and amyloid fibers as well as curli fibers produced by E. coli. It is also used in indirect identification measurement of c-di-GMP (48 (link), 49 (link)). E. coli W1688 and ΔycgF cell suspension samples were stained with 5 μL CR (25 mg/mL), which was followed by full-wavelength scanning to quantitatively analyze the amount of CR on the biofilm and further determine the amount of biofilm. TEM analysis facilitates high-resolution visualization of specific structural components, such as polysaccharide fibrils and environmental DNA (eDNA) (48 (link)). The two strains were cultured until an OD₆₀₀ value of 1 was recorded and then added to a 24-well plate containing fresh M63 medium and incubated under dark and blue light (450 nm; 1,300 lx) conditions for static culture at 30°C for 30 h. The bacteria were collected via centrifugation (12,000 × g, 2 min), resuspended, and washed with 1% PBS to prepare suspension samples. The suspension sample was dropped on a copper mesh with a support membrane and retained for 2 min; the excess liquid was absorbed from the edge of the droplet with filter paper. The copper mesh was inserted into the phosphotungstic acid dye solution and dyed for 15 s. After drying, the samples were observed under the Hitachi H-7700 TEM system.