Placement and removal of the E-VAC system was performed as following: A polyurethane foam sponge (pore size 400–600 μm; KCI, Wiesbaden Germany, Smith & Nephew, Hamburg, Germany) was adapted to the particular wound size as estimated by the endoscopist. The sponge size was required to be smaller than the wound cavity to promote collapse and subsequent closure of the fistula. The sponge was fixed to the tip of a duodenal tube with a mersilene suture (Freka Tube, 15 Ch; Fresenius Kabi, Bad Homburg, Germany; 0.35 mm; Johnson & Johnson, St-Stevens-Woluwe, Belgium). The sponge was grasped with grasping forceps (Olympus, Hamburg, Germany; Boston Scientific, Marlborough, Massachussets, United States) and introduced either into the necrotic cavity or in the colonic lumen under vision using a regular orthograde endoscope (Gif-Q165, Gif-Q180H, Gif-Q190; Olympus). Continuous or intermittent suction of 50 to 100 mm Hg was applied using a vacuum pump (KCI, Smith & Nephew). For sponge removal the suction was discontinued and the tube was grasped with grasping forceps close to the distal end pulled out of the wound cavity. The sponge was exchanged approximately twice a week until the base of the cavity appeared to be firmly closed or the cavity was completely epithelialized/granulated. All procedures were performed by or in the presence of an experienced endoscopist (> 200 colonoscopies/year).
Gif q165
Manufactured by Olympus
Sourced in United States, Japan
The GIF-Q165 is a high-quality optical microscope designed for laboratory use. It features a sturdy construction, a comfortable and ergonomic design, and advanced optics for precise observation and analysis.
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Lab products found in correlation
7 protocols using gif q165
1
Endoscopic Vacuum-Assisted Closure of Fistulas
2
Comprehensive Evaluation of Hereditary Hemorrhagic Telangiectasia
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Baseline demographic data, underlying diseases, Curaçao criteria, Epistaxis Severity Score (ESS), genetic testing, anemia-related variables, and GI involvement characteristics were collected. ESS is an online tool that quantifies epistaxis severity considering different parameters during the previous three months (30 (link)). Screening for HHT-related visceral involvement is described elsewhere (31 (link)). Anemia-related variables include: baseline hemoglobin (Hb) level at the beginning of follow-up, minimal Hb level during follow-up, severe anemia, IV iron therapy, RBC transfusion requirements, and number of RBC units transfused per patient. Minimal Hb level was selected instead of mean Hb level during follow-up because it better reflects the degree of anemia control and the severity of chronic bleeding episodes. Severe anemia was defined as minimal Hb levels <80 g/L.
GI evaluation was performed with esophagogastroduodenoscopy or colonoscopy (Olympus GIF-Q165) or endoscopic capsule (PillCamSB 3) in patients with disproportionate anemia to the amount and severity of epistaxis (4 (link)). Telangiectasias were classified according to their number (few: ≤ 10 telangiectasias or multiple: >10 telangiectasias) and size (small: ≤ 3 mm or large: >3 mm) (13 (link)). Previous use of APC therapy before SA treatment was also collected.
GI evaluation was performed with esophagogastroduodenoscopy or colonoscopy (Olympus GIF-Q165) or endoscopic capsule (PillCamSB 3) in patients with disproportionate anemia to the amount and severity of epistaxis (4 (link)). Telangiectasias were classified according to their number (few: ≤ 10 telangiectasias or multiple: >10 telangiectasias) and size (small: ≤ 3 mm or large: >3 mm) (13 (link)). Previous use of APC therapy before SA treatment was also collected.
3
Standardized Endoscopic Ultrasound Examinations
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All gastroscopies (Olympus GIF Q 180, GIF Q 165, GIF Q 145) were performed by endoscopists in the Endoscopy Laboratory of the Department of Gastroenterology. All procedures were performed by an endosonographer with formal training and expertise in endoscopic ultrasound. Standards for EUS examination were followed. Examinations were performed in planned mode. Patients remained in the fasting state before the examinations. The examinations were performed in the left lateral recumbent position. The patients who tolerated gastroscopy poorly underwent EUS with intravenous anaesthesia. A radial echoendoscope, a linear echoendoscope, or both (Olympus GF-UCT 160, Olympus GF-UM 20, Pentax EG-3870 UTK) were used for the examination as needed.
The study was conducted in accordance with the Declaration of Helsinki and was approved by the institutional review board as well by the Local Ethics Committees when required. All the patients provided informed consent.
The study was conducted in accordance with the Declaration of Helsinki and was approved by the institutional review board as well by the Local Ethics Committees when required. All the patients provided informed consent.
4
Endoscopic Ultrasound Examination Protocol
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All gastroscopies (Olympus GIF Q 180, GIF Q 165, GIF Q 145) were performed by endoscopists in the Endoscopy Laboratory of the Department of Gastroenterology. One EUS examiner was involved in this study. A radial echoendoscope (Olympus GF-UM 20) and/or a linear echoendoscope (Olympus GF-UCT 160, Pentax EG-3870 UTK) were used for the examination as required. All procedures were performed by an endosonographer with formal training and expertise in endoscopic ultrasonography. Before the examinations, patients remained in the fasted state. Standards for EUS examination were followed. Examinations were performed in the supine position on the left side. Intravenous anaesthesia was carried out in those patients who were known to badly endure gastroscopy. Those patients required access to the vein of the forearm.
5
Esophageal Injury Assessment Post Ablation
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The decision to perform EUS was made at the discretion of the operators based on potential risk factors of EI3 (link),13 (link)–17 (link), as well as clinical manifestation post ablation (such as fever, chest discomfort, elevated white blood cell count and C-reaction protein level).
EUS was performed by experienced operators to assess the magnitude of EI. EI was defined as any esophageal lesion adjacent to the contact area between the esophagus and the LA, and was defined according to Kansas City classification: type 1: erythema; type 2: ulcers (2a: superficial ulcers; 2b: deep ulcers); type 3: perforation (3a: perforation without communication with the atria; 3b: perforation with atrioesophageal fistula)18 (link). All gastroscopies (GIFQ 260, GIFQ 165, GIFQ 145; Olympus, Japan) were performed by endoscopists in an endoscopy laboratory. A radial echoendoscope (EU-ME2 PREMIER PLUS, Olympus, Japan) was used for examination. Careful examination of the mediastinum and esophageal wall was performed to assess mucosal and periesophageal/mediastinal lesions. Patients were kept fasting for at least 8 hours prior to the procedure. Examinations were performed in the left lateral decubitus position and under conscious sedation with propofol.
EUS was performed by experienced operators to assess the magnitude of EI. EI was defined as any esophageal lesion adjacent to the contact area between the esophagus and the LA, and was defined according to Kansas City classification: type 1: erythema; type 2: ulcers (2a: superficial ulcers; 2b: deep ulcers); type 3: perforation (3a: perforation without communication with the atria; 3b: perforation with atrioesophageal fistula)18 (link). All gastroscopies (GIFQ 260, GIFQ 165, GIFQ 145; Olympus, Japan) were performed by endoscopists in an endoscopy laboratory. A radial echoendoscope (EU-ME2 PREMIER PLUS, Olympus, Japan) was used for examination. Careful examination of the mediastinum and esophageal wall was performed to assess mucosal and periesophageal/mediastinal lesions. Patients were kept fasting for at least 8 hours prior to the procedure. Examinations were performed in the left lateral decubitus position and under conscious sedation with propofol.
6
Endoscopic Evaluation of Celiac Disease
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Esophagogastroduodenoscopy (EGDs) with at least four biopsies obtained from the second part of the duodenum was performed in all patients using a flexible video-gastro scope (Olympus GIF-Q165, GIFQ185). A subgroup of CD patients underwent endoscopic histologic re-evaluation in a period ranging from 12 to 30 months after beginning the GFD.
The same pathologist expert in the field of CD, who was blinded to clinical data, examined the intestinal biopsies for each patient both at diagnosis and follow-up. Biopsies were analysed after haematoxylin and eosin and immunohistochemical staining for CD3 counts and were assessed using the Marsh classification system modified by Oberhuber [16 (link),17 (link)]. The histological persistence of the above-listed alterations was described and classified as Marsh 1, Marsh 2, or Marsh 3 (A, B, or C), as previously defined.
The same pathologist expert in the field of CD, who was blinded to clinical data, examined the intestinal biopsies for each patient both at diagnosis and follow-up. Biopsies were analysed after haematoxylin and eosin and immunohistochemical staining for CD3 counts and were assessed using the Marsh classification system modified by Oberhuber [16 (link),17 (link)]. The histological persistence of the above-listed alterations was described and classified as Marsh 1, Marsh 2, or Marsh 3 (A, B, or C), as previously defined.
7
Celiac Disease Diagnosis and Follow-up Protocol
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EGDS with at least four biopsies obtained from the second part of the duodenum was performed in all patients using a flexible video-gastroscope (Olympus GIF-Q165, GIFQ185). All included patients underwent EGDS at diagnosis and histological re-evaluation in a period ranging from 12 to 28 months after beginning an adequate GFD.
The same expert pathologist in the field of CD (E.P.), who was blinded to clinical data, examined the intestinal biopsies for each patient both at diagnosis and follow-up. Biopsies were analyzed after hematoxylin and eosin and immunohistochemical staining for CD3 counts and were assessed using the Marsh classification system modified by Oberhuber [20 (link),22 (link)]. The histological persistence of the above-listed alterations was described and classified as Marsh 1, Marsh 2 or Marsh 3 (A, B or C), as previously defined. To assess the concomitant presence of Helicobacter pylori (H.p.) infection and/or other gastric diseases, at least five gastric biopsies (1 from the lesser and 1 from the greater curve of the antrum, 1 from angular incisura and 2 from the corpus/fundus) were taken at CD diagnosis and at the time of re-evaluation according to the updated Sydney system [23 (link)].
The same expert pathologist in the field of CD (E.P.), who was blinded to clinical data, examined the intestinal biopsies for each patient both at diagnosis and follow-up. Biopsies were analyzed after hematoxylin and eosin and immunohistochemical staining for CD3 counts and were assessed using the Marsh classification system modified by Oberhuber [20 (link),22 (link)]. The histological persistence of the above-listed alterations was described and classified as Marsh 1, Marsh 2 or Marsh 3 (A, B or C), as previously defined. To assess the concomitant presence of Helicobacter pylori (H.p.) infection and/or other gastric diseases, at least five gastric biopsies (1 from the lesser and 1 from the greater curve of the antrum, 1 from angular incisura and 2 from the corpus/fundus) were taken at CD diagnosis and at the time of re-evaluation according to the updated Sydney system [23 (link)].
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