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Molm 13

Manufactured by Leibniz Institute DSMZ
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Sourced in Germany, United States

MOLM-13 is a cell line derived from a patient with acute myeloid leukemia. It is a suspension-growing cell line that can be used for various cell biology and cancer research applications.

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Lab products found in correlation

Oci aml3, by Leibniz Institute DSMZ (31 mentions) Mv4 11, by Leibniz Institute DSMZ (25 mentions) Mv4 11, by American Type Culture Collection (18 mentions) Hl 60, by Leibniz Institute DSMZ (13 mentions) Thp 1, by American Type Culture Collection (13 mentions) Oci aml2, by Leibniz Institute DSMZ (12 mentions) Thp 1, by Leibniz Institute DSMZ (10 mentions) Nomo 1, by Leibniz Institute DSMZ (10 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (10 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (10 mentions) Kasumi 1, by Leibniz Institute DSMZ (9 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Mycoalert mycoplasma detection kit, by Lonza (7 mentions) Rpmi 1640, by Thermo Fisher Scientific (6 mentions) L glutamine, by Thermo Fisher Scientific (6 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions) Hek293t, by American Type Culture Collection (6 mentions) Penicillin, by Thermo Fisher Scientific (5 mentions) Streptomycin, by Thermo Fisher Scientific (5 mentions) Jurkat, by American Type Culture Collection (5 mentions)

Close spelling variants of this product

Molm-13 cells (8 citations) MOLM-13 cell line (3 citations)

84 protocols using molm 13

1

Cell Line Procurement and Culture

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HL-60 cells were obtained from NCI 60-Panel. Jurkat and MV4-11 cells were obtained from ATCC. OCI-AML5, RS4;11, SEM, ML-2, MOLM-13, MOLM14, NOMO-1, OCI-AML2, KOPN-8, EOL-1, and OCI-AML3 cells were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). All used cells were cultured in the appropriate media and conditions.
Brzezinka K., Nevedomskaya E., Lesche R., Steckel M., Eheim A.L., Haegebarth A, & Stresemann C. (2019). Functional diversity of inhibitors tackling the differentiation blockage of MLL-rearranged leukemia. Journal of Hematology & Oncology, 12, 66.
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2

AML Cell Lines for Therapeutic Development

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Since there is an urgent need to develop new therapeutics for AML with t (8; 21) translocation and the MLLr-AML, Kasumi-1 (M2 subtype of AML with t (8; 21) translocation, DSMZ: ACC 220), MOLM-13 (M5 subtype of AML with MLLr, DSMZ: ACC 554), and THP-1 (M5 subtype of AML with MLLr, DSMZ No.: ACC 16) cell lines were used. In addition, KG-1 was also used to assess the effect of I3 on the proliferaton of leukemic stem-like cells, which has leukemic stem cells characteristics and was established from the bone marrow cells of an AML patient (DSMZ: ACC 14). Kasumi-1, KG-1, MOLM-13, and THP-1 cells were purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Leibniz, Germany) and maintained in RPMI-1640 medium, supplemented with 10% or 20% FBS and 1% penicillin-streptomycin, and incubated at 37°C in 5% CO2.
Zhao M., Duan Y., Wang J., Liu Y., Zhao Y., Wang H., Zhang L., Chen Z.S., Hu Z, & Wei L. (2022). Histone Deacetylase Inhibitor I3 Induces the Differentiation of Acute Myeloid Leukemia Cells with t (8; 21) or MLL Gene Translocation and Leukemic Stem-Like Cells. Journal of Oncology, 2022, 3345536.
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3

Expression Analysis of KIF2A in AML

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Human AML cell lines, including KG-1 [American Type Culture Collection (ATCC)], Kasumi-1 (ATCC), MOLM-13 [Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ)] and OC-AML2 (DSMZ), were cultured with 90% RPMI-1640 supplemented with 10% fetal bovine serum (FBS; both Hyclone; Cytiva) according to the manufacturer's instructions. The cells were cultured in 5% CO2 at 37°C. The expression of KIF2A in AML cell lines was determined by RT-qPCR and western blotting using BMMCs from healthy subjects as the control.
Liang X, & Xia R. (2021). Kinesin family member 2A acts as a potential prognostic marker and treatment target via interaction with PI3K/AKT and RhoA/ROCK pathways in acute myeloid leukemia. Oncology Reports, 47(1), 18.
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4

Cell Line Characterization and Manipulation

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Cell lines used in the study included 293T (American Tissue Culture Collection [ATCC], CRL-3216), Hela (ATCC, CCL-2), MV4;11 (ATCC, CRL-9591), RS4;11 (ATCC, CRL-1873), MOLM-13 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ], ACC554), KOPN-8 (DSMZ, ACC552), EOL-1 (DSMZ, ACC386), K562 (ATCC, CRL-243), and THP-1 (ATCC, TIB-202). An MM1.S-derivative line with CRBN knockout (KO; CRBN-/-) was provided by Drs. J Brander and W Kaelin (Dana Farber Cancer Institute). Luciferase (luc)-labeled cells were generated by infection with MSCV-luciferase-IRES-neo retrovirus and subsequent selection. Luciferase expression was validated with Luciferase Assay System (Promega, #E1500). MV4;11 cells stably expressed with doxycycline (Dox)-inducible Cas9 (iCas9)61 were a gift of Drs. X Shi and H Wen (Van Andel Institute). Identity of cell lines was ensured by UNC’s Tissue Culture Facility with genetic signature analyses and examination of mycoplasma contamination performed using commercial detection kits (Lonza, #LT27–286).
Methods for cell transfection, generation of stable expression cell lines, assays for cell cycle progression, apoptosis and colony formation, as well as hematopoietic stem/progenitor cell (HSPC) purification and related colony formation unit (CFU) assay, were provided in Supplementary Note file.
Wang J., Yu X., Gong W., Liu X., Park K.S., Ma A., Tsai Y.H., Shen Y., Onikubo T., Pi W.C., Allison D.F., Liu J., Chen W.Y., Cai L., Roeder R.G., Jin J, & Greg Wang G. (2022). EZH2 non-canonically binds cMyc and p300 through a cryptic transactivation domain to mediate gene activation and promote oncogenesis. Nature cell biology, 24(3), 384-399.
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5

Cell Culture of MLL-r AML and HEK293T

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MLL‐r AML‐derived MOLM‐13 and MV4‐11 cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany), and American Type Culture Collection (ATCC, Manassas, VA, USA), respectively. These MLL‐r AML cell lines were cultured in Roswell Park Memorial Institute 1640 medium containing 10% heat‐inactivated FBS and 1% penicillin–streptomycin (PS) under 5% CO2 and 95% air at 37 °C. Embryonic kidney HEK293T cells were obtained from Japanese Collection of Research Bioresources (JCRB, Ibaraki, Osaka, Japan). HEK293T cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% PS under 5% CO2 and 95% air at 37 °C.
Noura M., Matsuo H., Koyama A., Adachi S, & Masutani H. (2020). TXNIP induces growth arrest and enhances ABT263‐induced apoptosis in mixed‐lineage leukemia‐rearranged acute myeloid leukemia cells. FEBS Open Bio, 10(8), 1532-1541.
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6

Establishing MLL-fusion AML Cell Lines

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Human MLL-fusion AML cell lines MV4;11 [37 (link)] and THP-1 [35 (link)] were from ATCC (American Type Culture Collection; VI, USA) and Molm-13 [36 (link)] from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen; Leibniz, Germany). Culture medium was RPMI 1640 + 10% FBS, with inclusion of 50 μM 2-mercaptoethanol for THP-1 cells.
Anstee N.S., Bilardi R.A., Ng A.P., Xu Z., Robati M., Vandenberg C.J, & Cory S. (2018). Impact of elevated anti-apoptotic MCL-1 and BCL-2 on the development and treatment of MLL-AF9 AML in mice. Cell Death and Differentiation, 26(7), 1316-1331.
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7

Cell Lines Acquisition and Culturing

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Cell lines were acquired from American Type Culture Collection (ATCC) or the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), as indicated: HEK293T cells (ATCC #CRL-3216), MOLM13 (DSMZ Cat#ACC554), MV4;11 (ATCC Cat#CRL-9591), OCI-AML2 (DSMZ Cat#ACC-99), THP-1 (ATCC Cat#TIB-202), OCI-AML3 (DSMZ Cat#ACC-582), U937 (ATCC Cat#CRL-1593.2), and HL60 (ATCC Cat#CCL-240). The IMS-M2 cell line (NPM1c) was a kind gift from Dr. Daniel Tenen, Harvard Medical School, with original source as previously described70 . Identification cell lines was independently confirmed by cytogenetics profiling. Routine mycoplasma testing was negative. Cells were cultured in RPMI 1640 (Gibco Cat #11875–093) supplemented with 10% foetal bovine serum, 100U/mL penicillin (Gibco), 100 μg/mL streptomycin (Gibco), and L-glutamine 2 mM at 37°C and 5% CO2.
Aubrey B.J., Cutler J.A., Bourgeois W., Donovan K.A., Gu S., Hatton C., Perlee S., Perner F., Rahnamoun H., Theall A.C., Henrich J.A., Zhu Q., Nowak R.P., Kim Y.J., Parvin S., Cremer A., Olsen S.N., Eleuteri N.A., Pikman Y., McGeehan G.M., Stegmaier K., Letai A., Fischer E.S., Liu X.S, & Armstrong S.A. (2022). IKAROS and MENIN Coordinate Therapeutically Actionable Leukaemogenic Gene Expression in MLL-r Acute Myeloid Leukaemia. Nature cancer, 3(5), 595-613.
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8

Cell Lines Acquisition and Culturing

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Cell lines were acquired from American Type Culture Collection (ATCC) or the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), as indicated: HEK293T cells (ATCC #CRL-3216), MOLM13 (DSMZ Cat#ACC554), MV4;11 (ATCC Cat#CRL-9591), OCI-AML2 (DSMZ Cat#ACC-99), THP-1 (ATCC Cat#TIB-202), OCI-AML3 (DSMZ Cat#ACC-582), U937 (ATCC Cat#CRL-1593.2), and HL60 (ATCC Cat#CCL-240). The IMS-M2 cell line (NPM1c) was a kind gift from Dr. Daniel Tenen, Harvard Medical School, with original source as previously described70 . Identification cell lines was independently confirmed by cytogenetics profiling. Routine mycoplasma testing was negative. Cells were cultured in RPMI 1640 (Gibco Cat #11875–093) supplemented with 10% foetal bovine serum, 100U/mL penicillin (Gibco), 100 μg/mL streptomycin (Gibco), and L-glutamine 2 mM at 37°C and 5% CO2.
Aubrey B.J., Cutler J.A., Bourgeois W., Donovan K.A., Gu S., Hatton C., Perlee S., Perner F., Rahnamoun H., Theall A.C., Henrich J.A., Zhu Q., Nowak R.P., Kim Y.J., Parvin S., Cremer A., Olsen S.N., Eleuteri N.A., Pikman Y., McGeehan G.M., Stegmaier K., Letai A., Fischer E.S., Liu X.S, & Armstrong S.A. (2022). IKAROS and MENIN Coordinate Therapeutically Actionable Leukaemogenic Gene Expression in MLL-r Acute Myeloid Leukaemia. Nature cancer, 3(5), 595-613.
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9

Cell Line Cultivation Protocols

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AML-derived THP-1 and KG-1a cells were purchased from RIKEN biological resource center (BRC, Japan). AML-derived Kasumi-1, HL60, SKNO-1, NOMO-1 cells and embryonic kidney derived HEK293T cells were from Japanese Collection of Research Bioresources (JCRB, Japan). AML-derived KO52, NB4, ME-1, ML-2, OCI-AML2, MV4-11, MOLM-13 and HEL cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Germany). HEK293T cells were maintained in Dulbecco's modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% Penicillin–Streptomycin (PS) in a humidified incubator with 5% CO2 and 95% air at 37 °C. The other cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% FBS and 1% PS under 5% CO2 and 95% air at 37 °C.
Noura M., Morita K., Kiyose H., Matsuo H., Nishinaka-Arai Y., Kurokawa M., Kamikubo Y, & Adachi S. (2020). Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Scientific Reports, 10, 20245.
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10

AML Cell Lines and Inhibitors

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Acute Myeloid Leukemia (AML) cell lines F-36P, HL-60, KASUMI-1, MOLM-13, MONO-MAC-6, MV-4-11, NB-4, NOMO-1, OCI-AML2, OCI-AML3, and THP-1 were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Germany). Cell lines were maintained at 37°C and 5% CO2 in appropriate media (Supplementary Table 1). 293T cells were cultured in DMEM (Gibco, Germany) along with 10% FBS (Biochrom, Germany). Cell lines were routinely verified for mycoplasma contamination using Venor®GeM Mycoplasma Detection Kit (Sigma-Aldrich, Germany). Cell lines were authenticated by Multiplexion, Germany. The inhibitors were purchased from Abcam (UK), Biozol (Germany), and Santa Cruz Biotechnology (US).
More P., Goedtel-Armbrust U., Shah V., Mathaes M., Kindler T., Andrade-Navarro M.A, & Wojnowski L. (2019). Drivers of topoisomerase II poisoning mimic and complement cytotoxicity in AML cells. Oncotarget, 10(51), 5298-5312.
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