Identification of P. aeruginosa was done on the basis of Gram staining, colony morphologies on MacConkey's agar, motility, pigment production, oxidase reaction, growth at 42°C, and the biochemical tests included in the API 20NE identification kit (Biomerieux, Marcy l'Étoile, France). The Vitek 2 system (Vitek 2 software, version R02.03; Advanced Expert System [AES] software, version R02.00N (bioMerieux, Marcy l'Étoile, France) was used with the ID-GNB card for identification of Gram-negative bacilli. The identified strains were stored in glycerol broth cultures at −70°C.
Id gnb card
Manufactured by bioMérieux
Sourced in France
The ID-GNB card is a laboratory diagnostic tool used for the identification of Gram-negative bacteria. It provides a rapid and reliable method for the identification of a wide range of bacterial species.
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Lab products found in correlation
6 protocols using id gnb card
1
Identification of Pseudomonas aeruginosa
2
Identification of Colistin-Resistant K. pneumoniae
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The colistin-resistant K. pneumoniae strains were identified using Vitek-2 and ID-GNB card for Gram-negative bacilli according to manufacturer's instructions (bioMérieux, Marcy l'Etoile, France).
3
Enterobacteriaceae Identification Protocol
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All the Enterobacteriaceae isolates were identified using the Vitek 2 automatic system and the ID-GNB card for identification of Gram-negative bacilli according to the manufacturer’s instructions (bio-Merieux, Marcy l’Etoile, France). Two Raoultella spp., and one Enterobacter spp. were further identified as R. planticola, R. ornithinolytica and E. aerogenes by amplification and sequencing of the rpoB gene as previously described [17 (link)].
4
Carbapenem-Resistant Enterobacteriaceae in Pediatric Cancer Hospital
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The epidemiology of carbapenem-resistant Enterobacteriaceae was studied in a 320-bed tertiary care hospital (Children’s Cancer Hospital) for pediatric cancer patients between November 2014 and August 2016, in Cairo, Egypt. A total of 149 non-duplicated Gram-negative isolates were collected. All isolates were identified by both biochemical testing and VITEK 2 automated system using the ID-GNB card for identification of Gram-negative bacilli according to the manufacturer’s instructions (bioMérieux, France). The study approval was obtained from the Research Ethics Committee of the Faculty of Pharmacy, Cairo University (approval no. MI-1203). No patients’ consent form was required as all the isolates were obtained from the hospital microbiology lab after being grown on MacConkey and Trypticase Soy Agar, for routine hospital work, then stored at −80°C in LB medium supplemented with 15% v/v glycerol.
5
Identification of Klebsiella pneumoniae
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Klebsiella pneumoniae isolates were identified using the Vitek 2 automatic system and the ID-GNB card according to the manufacturer’s instructions (bioMérieux, Marcy l’Etoile, France) as previously described (Del Franco et al., 2015 (link)).
6
Isolating Pseudomonas aeruginosa from Burn Wounds
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The standard method developed by Zgair et al. was followed. 9 Wound swabs were collected from 110 patients suffering from burn wound infections and treated at the Baghdad Teaching Hospital in Baghdad, Iraq. All individuals gave written informed consent to participate in the study. The patients did not receive any antibiotics for 2 days before sample collection. The swabs were subjected to an asparagine broth enrichment medium to enhance P. aeruginosa growth, and incubated for 48 h at 37°C with vigorous shaking at 200 rpm. A loopful of bacterial suspension was streaked onto asparagine plates containing 1.5% agar (HiMedia, Mumbai, India) and incubated at 37°C until colonies developed. 9 A VITEK 2 DensiCHEK fluorescence instrument system with an ID-GNB card (bioMérieux, Marcy-l'Étoile, France) was used to identify the isolates of P. aeruginosa. 10 (link) The study was conducted following approval from the Human Ethical Committee of the University of Baghdad, Iraq (Reference No. HS-212, April 1, 2020).
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