Amylase lq

Lipase, amylase and aspartate aminotransferase (AST) activity were determined in plasma by the LIPASE-LQ, AMYLASE-LQ and GOT (AST)-LQ, respectively, (Spinreact, Girona, Spain). The procedures were performed according to the indications of the kit.
Triglycerides and total lipids were determined in liver tissue by the TRIGLYCERIDES-LQ and TOTAL LIPIDS, respectively (Spinreact, Girona, Spain). The procedures were performed according to the indications of the kit in liver homogenate in PBS (100 mg/mL). Results were normalized by protein concentration.

The α-amylase-inhibitory activity was assessed by using a commercial kit (SPINREACT, ref Amylase-LQ, Girona, Spain). The formation rate of 2-chloro-4-nitrophenol, which was observed photometrically, is positively correlated with the catalytic concentration of α-amylase present in the sample. Then 80 μL of protein hydrolysate solution was added to 40 μL of α-amylase enzyme (from porcine pancreas, A3176, 1 μU), and they were mixed accordingly. Then the reaction mixture was incubated at 37 °C, for 10 min. After that, 160 μL of the 2-chloro-4-nitrophenyl-α-D-maltotrioside (CNPG3) containing ((MES pH = 6, 100 mM), (CNPG3, 2.25 mM), (sodium clorhidre, 350 mM), (calcium acetate, 6 mM), (potassium thiocyanate, 900 mM), (sodium azide 0.95 gr/L)) was added, and then the reaction mixture was stored at 37 °C, for 5 min. Finally, the optical density was recorded at 405 nm. Phosphate buffer 20 mM (pH 6.8) and Acarbose (2 mg/mL phosphate buffer) were considered as blank and positive controls, respectively (SPINREACT, Girona, Spain). The α-amylase-inhibitory activity was determined as follows: