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Application suite v3

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The Application Suite V3 software is a comprehensive platform designed to provide users with a robust and intuitive interface for controlling and managing Leica laboratory equipment. The software offers a unified and integrated solution for seamless operation of Leica's imaging and analysis systems.

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Lab products found in correlation

Dm2500 microscope, by Leica (7 mentions) Dfc420 camera, by Leica (5 mentions) Dhc490, by Leica (5 mentions) Dfc420c camera, by Leica (4 mentions) Dfc420, by Leica (3 mentions) Dfc310 fx, by Leica (3 mentions) Icc50 hd camera, by Leica (3 mentions) Matrigel, by BD (3 mentions) Dfc425c, by Leica (3 mentions) Shandon excelsior, by Thermo Fisher Scientific (3 mentions) Dmrbe microscope, by Leica (3 mentions) Mz10f microscope, by Leica (2 mentions) Gentamycin, by Thermo Fisher Scientific (2 mentions) Axiovision 4, by Zeiss (2 mentions) Axioscope 2, by Zeiss (2 mentions) Axiocam mrc5, by Zeiss (2 mentions) Dm2500, by Leica (2 mentions) Dfc 300 fx digital camera, by Leica (2 mentions) Dmi4000b inverted microscope, by Leica (2 mentions) M205 fa, by Leica (2 mentions)

85 protocols using application suite v3

1

Histological Analysis of Testicular Tissue

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Testes were fixed in Bouin solution overnight and embedded in paraffin. Five μm thick sections were stained with Periodic-Acid-Schiff and analysed using a MZ9.5 (Leica) microscope coupled with a DHC490 (Leica) camera and application suite V3.3.0 (Leica) software and processed with Adobe Photoshop. Macroscopic views were performed with a MZ16 (Leica) microscope coupled with a DHC490 (Leica) camera and application suite V3.3.0 (Leica) software and processed with Adobe Photoshop.
Gregoire E.P., Stevant I., Chassot A.A., Martin L., Lachambre S., Mondin M., de Rooij D.G., Nef S, & Chaboissier M.C. (2018). NRG1 signalling regulates the establishment of Sertoli cell stock in the mouse testis. Molecular and cellular endocrinology, 478.
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2

Iodine Staining Distinguishes Sporulation in Fission Yeast

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Iodine vapour stains spores but not vegetative S. pombe cells (Forsburg and Rhind 2006 (link)). On sporulation medium, colonies of a switching competent h90 wild-type strain turn homogeneously brown when stained with iodine, while colonies of non-sporulating heterothallic strains are yellow. h90msh3 mutants form mottled colonies and frequently segregate iodine-negative h+ colonies due to duplications in the mating-type region (Egel et al. 1984 (link); Fleck et al. 1990 (link)). Images of iodine-stained colonies were taken with a LEICA MZ10F microscope using LEICA Application Suite V3 software. To determine the frequency of heterothallic colonies, three h90 colonies of each strain were streaked on MEA and grown for 3 days at 30 °C. From each original h90 colony, approximately 300–500 colonies were inspected after iodine staining.
Villahermosa D., Knapp K, & Fleck O. (2017). A mutated dph3 gene causes sensitivity of Schizosaccharomyces pombe cells to cytotoxic agents. Current Genetics, 63(6), 1081-1091.
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3

Oligodendrocyte Precursor Cell Migration

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OPCs were seeded on pFn or aFn coated‐polyethylene terephthalate membranes of 8 µm pore size (Becton‐Dickinson Labware) in 12‐well modified Boyden transwell microchambers at a density of 80,000 cells per insert. OPCs were allowed to migrate through the membranes for 4 h using 10 ng/mL PDGF‐AA as a chemoattractant in the bottom of the well. Nonmigrating cells were removed from the top compartment with a cotton swab. Remaining cells in the membranes were fixed for 20 min in 5% acetic acid in ethanol and nuclei were visualized using DAPI (1 µg/mL) in PBS for 30 min. After washing in PBS, membranes were mounted on glass slides and images of DAPI‐positive, migrated cells were captured using a Zeiss Axioskop 2 plus microscope with Leica Application Suite v3 software (15 × 20 fields per membrane). The numbers of cells were assessed using FIJI software.
Stoffels J.M., Hoekstra D., Franklin R.J., Baron W, & Zhao C. (2014). The EIIIA domain from astrocyte‐derived fibronectin mediates proliferation of oligodendrocyte progenitor cells following CNS demyelination. Glia, 63(2), 242-256.
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4

Stereomicroscopy Image Capture and Visualization

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Images were taken using a Leica MZ FLII stereomicroscope and the Leica Application Suite V3 software. Contrast and image size were adjusted with Adobe Photoshop CS5.1. All bar plots were generated using GraphPad Prism 7. All figures were generated with Adobe Photoshop or Adobe Illustrator.
Gamart J., Barozzi I., Laurent F., Reinhardt R., Martins L.R., Oberholzer T., Visel A., Zeller R, & Zuniga A. (2021). SMAD4 target genes are part of a transcriptional network that integrates the response to BMP and SHH signaling during early limb bud patterning. Development (Cambridge, England), 148(23), dev200182.
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5

Prussian Blue Staining of Heart Valves

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Prussian blue reaction involves the treatment of sections with acid solutions of ferrocyanides. Any ferric ion (Fe3+) present in the tissue combines with the ferrocyanide and results in the formation of a bright blue pigment called Prussian blue, or ferric ferrocyanide. Heart valve tissues were fixed with 10 % neutral buffered formaldehyde for two days followed by decalcination (EDTA-TRIS buffer) and embedded in paraffin wax. 4 μm slides were then deparaffinized for further staining. For special staining, slides were incubated in 5% potassium ferrocyanide and 5% hydrochloric acid containing solution for 30 minutes in fume hood. Nucleus staining was performed in Gallego solution for 5 minutes and samples were dehydrated. The intensity and distribution of ferrocyanide complex were assessed by light microscopy (Leica DM2500 microscope, DFC 420 camera and Leica Application Suite V3 software, Wetzlar, Germany).
Sikura K.É., Potor L., Szerafin T., Zarjou A., Agarwal A., Arosio P., Poli M., Hendrik Z., Méhes G., Oros M., Posta N., Beke L., Fürtös I., Balla G, & Balla J. (2019). POTENTIAL ROLE OF H-FERRITIN IN MITIGATING VALVULAR MINERALIZATION. Arteriosclerosis, thrombosis, and vascular biology, 39(3), 413-431.
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6

Immunohistochemical Analysis of CSE and SMA in Fixed Tissues

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Briefly, tissues were fixed in formaldehyde for 1 day followed by Tris buffer and embedded in paraffin wax. Subsequently, slides were deparaffinized in xylene and then rehydrated. For immunohistochemistry, slides were subjected to the peroxidase‐blocking reagent. Samples were incubated with the following primary antibodies: anti‐CSE antibody at dilution 1:1,000 (Proteintech Group Cat# 12217‐1‐AP, RRID:AB_2087497) and anti‐SMA antibody at a dilution of 1:1,000 (Santa Cruz Biotechnology Cat# sc‐32251, RRID:AB_262054). Antibody binding was visualized by the Super Sensitive™ One Step Polymer‐HRP IHC Detection System. Liquid DAB chromogen (BG‐QD630‐XAKm BioGenex) was added for samples. The intensity and distribution of antibodies expression were assessed by light microscopy (Leica DM2500 microscope, DFC 420 camera, and Leica Application Suite V3 software, Wetzlar, Germany).
Sikura K.É., Potor L., Szerafin T., Oros M., Nagy P., Méhes G., Hendrik Z., Zarjou A., Agarwal A., Posta N., Torregrossa R., Whiteman M., Fürtös I., Balla G, & Balla J. (2019). Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease. British Journal of Pharmacology, 177(4), 793-809.
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7

DHEA Effect on C. elegans Morphology

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C. elegans were cultivated and treated with or without the DHEA solutions from sweet potatoes as in the life-span assay described above. The day when the nematodes first treated with DHEA was considered as day 0. Picked one of the nematodes in good condition in each well to take photos on the 3rd, 6th and 9th days under utilization of Leica Application suite V3 software (version 3.40) . Then used Photoshop software to measure the length and width of the nematode. All determinations were replicated three times.
Chen H., Zou D., Wang L., & Huang J. (2020). Evaluation of the effect of life extension of DHEA extract from sweet potato on Caenorhabditis elegans.
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8

Termite Collection and Imaging Methodology

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Termites were aspirated from hatchet-exposed dead logs, branches, and fence posts and preserved in 85% ethanol. All samples are housed in the University of Florida Termite Collection (UFTC), Davie, Florida. Soldiers and the imago of I. platycephalus were photographed as multilayer montages using a Leica M205C stereomicroscope controlled by Leica Application Suite v. 3 software (Figs 1A,C,2A,C). The I. platycephalus locality map was prepared using ArcMap v. 10.3.
Scheffrahn R.H. (2020). New records of the drywood termite, Incisitermes platycephalus (Light, 1933) (Isoptera, Kalotermitidae), from Central America and senior synonym of I. nigritus (Snyder, 1946). Check List, 16(2), 501-505.
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9

DHEA Impacts on C. elegans Lifespan

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The worms were cultivated on the 96-well plates with various concentrations of DHEA or S. Medium as the control group, as mentioned in the life-span assay. The microscope and Leica Application Suite V3 software (version 3.40) was applied to determine the pharyngeal pumping rate every 20s of C. elegans on the 3rd, 6th and 9th days. The experiment was repeated three times.
Chen H., Zou D., Wang L., & Huang J. (2020). Evaluation of the effect of life extension of DHEA extract from sweet potato on Caenorhabditis elegans.
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10

Corneal Cell Viability Imaging

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The corneas were kept at 37°C in the chamber for 2 hours, then removed, rinsed with normal saline buffer and observed using fluorescence microscopy on a DMI4000B fluorescence microscope (Leica). The corneas were subsequently still kept in vials with Steinhardt medium at 37°C for 24 hours, prior to further fluorescence microscopy observation. The staining of cell nuclei was performed by incubating the corneas in 1 µM DAPI in PBS for 30 minutes endothelial side up, prior to observation. Images were acquired and merged using the Leica Application Suite V3 software.
Vicente-Pascual M., Albano A., Solinís M.Á., Serpe L., Rodríguez-Gascón A., Foglietta F., Muntoni E., Torrecilla J., Pozo-Rodríguez A.D, & Battaglia L. (2018). Gene delivery in the cornea: in vitro & ex vivo evaluation of solid lipid nanoparticle-based vectors. Nanomedicine (London, England), 13(15).
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