MGC‐803, HGC‐27 (purchased from the Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences, Shanghai, China), and THP1 (iCell Bioscience Inc, Shanghai, China) were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin. HLECs were obtained from ScienCell Research Laboratories (ScienCell, San Diego, CA). HLECs were cultured in endothelial cell medium (ScienCell, San Diego, CA) supplemented with 10% fetal bovine serum, 1% endothelial cell growth supplement, and 1% antibiotic solution. All cells were cultured in a humidified incubator at 37 °C with 5% CO2 (Thermo, Waltham, MA). None of the cell lines were listed in the database of commonly misidentified cell lines maintained by the International Cell Line Authentication Committee (ICLAC). All cell lines were routinely tested for mycoplasma contamination and were all free of contamination.
Hlecs
Manufactured by ScienCell
Sourced in United States
Human Lung Endothelial Cells (HLECs) are primary cells derived from human lung tissue. They are essential for the study of lung physiology and pathology.
Automatically generated - may contain errors
Lab products found in correlation
Endothelial cell medium (ecm), by ScienCell (9 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (7 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions)
Trypsin edta, by Thermo Fisher Scientific (4 mentions)
Egm 2 mv singlequot kit, by Lonza (3 mentions)
Huvecs, by Lonza (3 mentions)
Umuc3, by American Type Culture Collection (3 mentions)
Ms751, by American Type Culture Collection (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Sv huc 1, by American Type Culture Collection (2 mentions)
Um uc 3, by Thermo Fisher Scientific (2 mentions)
Endothelial cell growth supplement (ecgs), by ScienCell (2 mentions)
Fetal bovine serum (fbs), by Avantor (2 mentions)
Human broblasts, by Thermo Fisher Scientific (2 mentions)
T24 and 5637 human bca cell, by American Type Culture Collection (2 mentions)
Penicillin, by ScienCell (2 mentions)
Streptomycin, by ScienCell (2 mentions)
Me 180, by American Type Culture Collection (2 mentions)
Pen strep, by Thermo Fisher Scientific (2 mentions)
41 protocols using hlecs
1
Cell Culture Protocols for Cancer and Immune Cell Lines
2
Cell Culture of Human Lung Cells
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Normal human bronchial epithelial cell line BEAS-2B was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), together with the following human NSCLC cell lines: A549, HCC827, NCI-H1650, NCI-H1299, NCI-H1703, NCI-H520, and NCI-H460. HLECs were obtained from ScienCell Research Laboratories (San Diego, California, USA).
BEAS-2B cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, catalog no. C11995500BT). A549 cells were cultured in Ham’s F-12K culture medium (Gibco, catalog no. 21127022), while the other NSCLC cell lines were cultured in RPMI 1640 culture medium (Gibco, catalog no. C11875500BT). Medium for all these cell lines was supplemented with 10% fetal bovine serum (FBS; BI, catalog no. 04-001-1ACS). HLECs were cultured in endothelial cell medium (ECM) supplemented with 5% FBS (ScienCell Research Laboratories, catalog no. 1001). All cultures were maintained at 37 °C in an incubator containing humidified air and atmosphere of 5% CO2.
BEAS-2B cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, catalog no. C11995500BT). A549 cells were cultured in Ham’s F-12K culture medium (Gibco, catalog no. 21127022), while the other NSCLC cell lines were cultured in RPMI 1640 culture medium (Gibco, catalog no. C11875500BT). Medium for all these cell lines was supplemented with 10% fetal bovine serum (FBS; BI, catalog no. 04-001-1ACS). HLECs were cultured in endothelial cell medium (ECM) supplemented with 5% FBS (ScienCell Research Laboratories, catalog no. 1001). All cultures were maintained at 37 °C in an incubator containing humidified air and atmosphere of 5% CO2.
3
KRAS-Mutant Pancreatic Cell Lines
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Human PDAC cell lines (KRASG12D: PANC-1, AsPC-1; KRASG12V: Capan-2; KRASG12C: Mia-PaCa2; KRASWT: BxPC-3) were obtained from American Type Culture Collection (ATCC). HPDE cells were obtained from Binsui Biotechnology. The HLECs were obtained from ScienCell Research Laboratories. The PANC-1 (ATCC, CRL-1469MET; RRID: CVCL_A4BT) and Capan-2 cells (ATCC, HTB-80; RRID: CVCL_0026) were maintained in DMEM (Invitrogen) containing 10% FBS. The AsPC-1 (ATCC, CRL-1682; RRID: CVCL_0152), BxPC-3 (ATCC, CRL-1687; RRID: CVCL_0186), Mia-PaCa2 (ATCC, CRM-CRL-1420; RRID: CVCL_0428), and HPDE cells (ATCC, HTX1979C) were maintained in RPMI 1640 medium (Invitrogen) containing 10% FBS. The HLECs (ScienCell Research Laboratories, 2500) were maintained in endothelial cell medium (ScienCell Research Laboratories) supplemented with 5% FBS. All cells were cultured at 37°C in humidified air with 5% CO2.
4
Bladder Cancer Cell Lines Cultivation
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The human urinary bladder transitional cell carcinoma cell lines T24 (RRID: CVCL_0554), UM-UC-3 (RRID: CVCL_1783) were purchased from ATCC, RT112 (RRID: CVCL_1670) was purchased from German Collection of Microorganisms and Cell Cultures GmbH (DSMZ) and UM-UC-1 (RRID: CVCL_2743) was purchased from European Collection of Authenticated Cell Cultures (ECACC). Human normal bladder epithelial cell line SV-HUC-1 (RRID: CVCL_3798) was purchased from ATCC. Murine transitional cell carcinoma cell line MB49 (RRID: CVCL_7076) was purchased from Millipore, and murine bladder epithelial cells (MBEC) were purchased from Procell (catalog no. CP-M058). Human lymphatic endothelial cells (HLEC) were obtained from ScienCell Research Laboratories. All cells were maintained in a humidified incubator with 5% CO2 at 37°C. The T24, RT112, and UM-UC-1 cells were cultured in RPMI 1640 medium (Gibco, catalog no. C11875500BT). The UM-UC-3 and MB49 cells were cultured in DMEM (Gibco, catalog no. C11995500BT). The SV-HUC-1 cells were cultured in Ham's F12K medium (Gibco, catalog no. 21127022). All medium was supplemented with 10% FBS (BI, catalog no. 04–001–1ACS). The HLECs were cultured in endothelial cell medium (ECM) with 5% FBS (ScienCell Research Laboratories, catalog no. 1001). The authentication and Mycoplasma testing of all cell lines were qualified.
5
Culturing and Characterizing Primary Human Lymphatic Endothelial Cells
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Primary hLECs (ScienCell Research Laboratories) were cultured according to the supplier’s instructions up to passage 5. hLECs were seeded at a density of 5000 cells per cm2 into T-75 flasks that had been coated with fibronectin (Sigma-Aldrich) overnight at 37 °C. hLECs were incubated at 37 °C with 5% CO2 overnight in endothelial cell medium (ECM) (ScienCell Research Laboratories) supplemented with 5% (v/v) foetal bovine serum (ScienCell Research Laboratories) and 1% (v/v) endothelial cell growth supplement (ScienCell Research Laboratories). The cells were then washed with phosphate buffered saline (PBS) and the culture medium replaced. The cells were grown until 90% confluent, detached from the flask using 2.5 mL 0.25% Trypsin-EDTA (Gibco) and used for experimentation (or passaged into new flasks). For confocal microscopy, 10,000 hLECs in 300 μL with complete ECM were seeded onto fibronectin-treated 1.5-thickness 10-mm-diameter glass coverslips (Glaswarenfabrik Karl Hecht) placed into each well of 24-well plates treated with tissue culture. For electron microscopy, 300,000 hLECs were seeded in 5 mL with complete ECM in fibronectin-treated T-25 flasks.
6
Culturing Human Lymphatic Endothelial Cells
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Human lymphatic endothelial cells (HLECs) were purchased from the ScienCell™ Research Laboratories and cultured in endothelial cell medium (ECM) containing 5 % fetal bovine serum (FBS), 1 % endothelial cell growth supplement (ECGS), 100 IU/mL penicillin and 100 μg/mL streptomycin. Lewis lung carcinoma LL/2 cells were obtained from American Type Culture Collection (ATCC) and cultured in DMEM containing 10 % FBS, 100 IU/mL penicillin and 100 μg/mL streptomycin. Subcultures were performed with trypsin-EDTA. All cells were incubated in an atmosphere of 5 % CO2 at 37 °C.
7
Cell Culture Protocols for Cancer Research
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Seven CCa cell lines, including Siha, HeLa, Caski, MS751, ME180, C33A and HeLa229, and normal cervix derived cell line H8 were purchased from ATCC and cultured in a humidified atmosphere with 5% CO2 at 37 °C. Human lymphatic endothelial cells (HLECs) were obtained from ScienCell Research Laboratories and maintained in the recommended endothelial cell medium (ScienCell, CA). All cell lines were cultured in complete medium with 10% FBS (Gibco, USA) as previously described31 (link).
8
Culturing Human Lymphatic Endothelial Cells
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Human lymphatic endothelial cells (HLECs, ScienCell, 2500) were cultured in standard T75 cm2 cell culture flasks (Corning, CLS430641U) coated with fibronectin as previously described [30 ,31 ] at a starting cell concentration of 5·105 cells. Cultures were maintained in endothelial basal medium-2 (Lonza, CC-3156) with EGM-2 MV SingleQuot Kit (Lonza, CC-4147), hereafter referred to as endothelial media. HLECs were cultured to 90-95% confluency at passage 3 for all experiments. Primary fibroblasts were routinely cultured in DMEM (Gibco, 11965092) with 10% fetal bovine serum (FBS, VWR, 97068-085), 1% Pen/Strep (ThermoFisher, 15140-122), and 1 µg/mL hydrocortisone (STEMCELL Technologies Inc., 07925). Primary human normal oral fibroblasts (HOrF, ScienCell, 2640) were cultured in standard T75 cm2 cell culture flasks (Corning, CLS430641U) at a starting cell concentration of 5·105 cells in fibroblast media (ScienCell, 2301). All cultures were kept in a humidified incubator at 37°C with 5% CO2. Media was refreshed every 2-3 days, and cells were cultured to 90-95% confluency.
9
Cell Culture of Bladder Cancer and Lymphatic Endothelial Cells
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Bladder cancer cells SW780 and UM-UC-3 were bought from the American Type Culture Collection (ATCC, USA) and grown in Dulbecco’s modified Eagle medium (Invitrogen, Carlsbad, California, USA) with fetal bovine serum (10%; HyClone, Logan, Utah, USA). Human lymphatic endothelial cells (HLECs) were acquired from ScienCell Research Laboratories (Carlsbad, California, USA) and maintained based on the manufacturer’s directions. Cell incubation was done at 37 °C in a 95% air and carbon dioxide (5%) environment.
10
Cell Culture Protocol for HeLa, SiHa, MS751 and HLECs
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