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Dnajb1

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DNAJB1 is a protein that functions as a molecular chaperone, assisting in the folding and unfolding of other proteins. It is involved in the regulation of the heat shock response and plays a role in protein quality control.

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Lab products found in correlation

Ph s675 β catenin, by Cell Signaling Technology (2 mentions) Ph ser33 37 thr41 β catenin, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) Cyclin d1, by Santa Cruz Biotechnology (2 mentions) Hace1, by Abcam (1 mentions) Streptavidin beads, by Merck Group (1 mentions) Hsp90, by R&D Systems (1 mentions) V5 peptide, by Merck Group (1 mentions) 3xflag peptide, by Merck Group (1 mentions) Cyp3a4, by Santa Cruz Biotechnology (1 mentions) Sparc, by Abcam (1 mentions) Col3a1, by Cell Signaling Technology (1 mentions) Thy 1, by Santa Cruz Biotechnology (1 mentions)

3 protocols using dnajb1

1

Comprehensive Evaluation of Wnt/β-Catenin Pathway

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β-catenin (Abcam; (E247) ab32572); ph-S675-β-catenin (Cell Signaling Tech; #4176S), ph-Ser33/37/Thr41-β-catenin (Cell Signaling Tech; #9561), TCF4 (Cell Signaling, #C48H11), p300 (Invitrogen; sc: PAI-848), HDAC1 (EDM Millipore Corp. 2E10), DNAJB1 (Abcam; ab69402), p21 (Santa Cruz; sc: F5), PGAP1 (Invitrogen; sc-PA5 72340), RUNDC1 (Abcam; sc-AB 151583), HACE1 (Abcam; sc-133637), cyclin D1 (Santa Cruz; sc-753), cdc2 (Santa Cruz; sc-954), β-actin (Sigma; A5316), polyclonal GPC3 (Abcam, ab18687), monoclonal GPC3 (Abcam, ab216606), AFP (Santa Cruz, C3), cdk2 (Transduction Laboratory, C18520), and cdk4 (Abcam, ab7955).
Gulati R., Hanlon M.A., Lutz M., Quitmeyer T., Geller J., Tiao G., Timchenko L, & Timchenko N. (2022). Phosphorylation-Mediated Activation of β-Catenin-TCF4-CEGRs/ALCDs Pathway Is an Essential Event in Development of Aggressive Hepatoblastoma. Cancers, 14(24), 6062.
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2

Affinity Purification of Tagged Proteins

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FLAG- or V5-tagged proteins were immunoprecipitated from cell lysate by incubation with FLAG- or V5-conjugated agarose beads for 1 hour (Sigma). For chemical precipitation, b-C86, GA beads, or PU-H71 beads (synthesized as described previously (34 (link), 35 (link))) were incubated with recombinant Hsp40 (DnaJB1, Abcam), Hsp70 (provided by Sue Wickner, NCI), Hsp90 (R&D Systems) or cell lysate for 1 hour, followed by streptavidin beads (Sigma) for 1 hour. In each case, beads were then washed at least 3 times with TNES buffer and bound protein eluted with 3xFLAG peptide (Sigma), V5 peptide (Sigma), or 2X gel loading buffer. Samples were analyzed by SDS-PAGE and WB.
Moses M.A., Kim Y.S., Rivera-Marquez G.M., Oshima N., Watson M.J., Beebe K.E., Wells C., Lee S., Zuehlke A.D., Shao H., Bingman WE I.I.I., Kumar V., Malhotra S.V., Weigel N.L., Gestwicki J.E., Trepel J.B, & Neckers L.M. (2018). Targeting the Hsp40/Hsp70 chaperone axis as a novel strategy to treat castration-resistant prostate cancer. Cancer research, 78(14), 4022-4035.
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3

Immunohistochemical Analysis of FLC Samples

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Liver and tumor sections from patients with FLC were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned (7‐μm sections). Immunohistochemistry for PKAc, ph‐S675‐β‐catenin, ki67, and post‐GPI attachment to proteins (PGAP1) was performed on FLC samples from primary liver tumors and lung metastasis.
List of antibodies used in this study: ph‐S675‐β‐catenin (Cell Signaling Tech; #D2F1),TCF4 (Cell Signaling; #C48H11), p300 (Invitrogen; sc: PAI‐848), Col3A1 (Cell Signaling Tech; #30565), secreted protein acidic and cysteine rich (SPARC) (Abcam; ab203284), Thy1 (Santa Cruz; sc: H‐110), versican (VCAN) (Invitrogen; MA5‐34654), HDAC1 (EDM Millipore Corp.; 2E10), DNAJB1 (Abcam; ab69402), E2F1 (Santa Cruz; sc:C20), p21 (Santa Cruz; sc: F5), PGAP1 (Invitrogen; sc‐PA5 72,340), RUN domain‐containing 1 (RUNDC1) (Abcam; sc‐AB 151583), HECT‐type E3 ubiquitin transferase (HACE1) (Abcam; sc‐133,637), cyclin D1 (Santa Cruz; sc‐753), cdc2 (Santa Cruz; sc‐954), CYP3A4 (Santa Cruz; sc‐30,621), β‐actin (Sigma; A5316), ph‐Ser33/37/Thr41‐β‐catenin (Cell Signaling; #8561), and ph‐Tyr333‐β‐catenin (Bioss; #12856R‐CY3).
Gulati R., Johnston M., Rivas M., Cast A., Kumbaji M., Hanlon M.A., Lee S., Zhou P., Lake C., Schepers E., Min K., Yoon J., Karns R., Reid L.M., Lopez‐Terrada D., Timchenko L., Parameswaran S., Weirauch M.T., Ranganathan S., Bondoc A., Geller J., Tiao G., Shin S, & Timchenko N. (2022). β‐catenin cancer–enhancing genomic regions axis is involved in the development of fibrolamellar hepatocellular carcinoma. Hepatology Communications, 6(10), 2950-2963.
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