Phospho p38 mapk thr180 tyr182 rabbit mab

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb is a highly specific antibody that recognizes p38 MAPK when phosphorylated at Thr180 and Tyr182. This antibody can be used to detect and quantify the levels of activated p38 MAPK in various cell and tissue samples.

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4 protocols using phospho p38 mapk thr180 tyr182 rabbit mab

Lentiviral Knockdown of PNPLA7 in Mice

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WT C57BL/6 mice aged from 6 to 8 weeks were obtained from the Experimental Animal Center of Chongqing Medical University. The lentiviral pLVX IRES Puro constructs to express GFP and PNPLA7-GFP Mission^® lentiviral pLKO.1 vectors encoding for scrambled shRNA or shRNAs targeting murine PNPLA7 were maintained in our lab [18 (link)]. LPS from Escherichia coli O111:B4 was obtained from Sigma. The MiniBEST Universal RNA Extraction Kit, PrimeScript™ II 1st Strand cDNA Synthesis Kit, and TB Green^® Fast qPCR Mix were purchased from TaKaRa. A PNPLA7 rabbit antiserum was generated in our lab by immunizing rabbits with a peptide in the regulatory domain of mouse PNPLA7. The generation of this antiserum and its specificity have been previously described [17 (link)]. The SIRT1 mouse monoclonal antibody (mAb) was purchased from Santa Cruz Biotechnology and β-actin mouse mAb, α-Tubulin mouse mAb, GAPDH mouse mAb, NF-κB p65 rabbit mAb, NF-κB1 p105/p50 rabbit mAb, iNOS antibody (Mouse Specific), IκBα (44D4) Rabbit mAb, p38 MAPK rabbit mAb, and Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb were obtained from Cell Signaling Technology. Anti-SOCS1 antibody and Anti-NF-κB p65 (acetyl K310) antibody were obtained from Abcam. Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor^® 647 conjugate was purchased from Thermo Fisher Scientific.

Zhao Z., Heier C., Pang H., Wang Y., Huang F, & Chang P. (2022). The Patatin–Like Phospholipase Domain Containing Protein 7 Regulates Macrophage Classical Activation through SIRT1/NF-κB and p38 MAPK Pathways. International Journal of Molecular Sciences, 23(23), 14983.

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Western Blot Antibody Panel for Signaling Pathways

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The following antibodies were used for western blot analysis: Rabbit anti-rat ANX A1 (ab65844) was obtained from Abcam (Cambridge, USA); and phospho-p44/42 MAPK (Thr202/Tyr204) rabbit mAb (catalogue no. 4370); p44/42 MAPK rabbit mAb (catalogue no. 4695); phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (catalogue no. 4511); p38 MAPK rabbit mAb (catalogue no. 8690); and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) rabbit mAb (catalogue no. 5174) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). All the primary antibodies were used at a dilution of 1:1,000. The secondary anti-rabbit IRDye 800CW antibody was obtained from LI-COR Biosciences (Lincoln, NE, USA).

PAN X., PENG L, & YIN G. (2015). Downregulation of Annexin A1 by short hairpin RNA inhibits the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. International Journal of Molecular Medicine, 36(2), 406-414.

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Protein Profiling by Western Blotting

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Western blotting was performed as described previously (Chen et al. 2013b (link); Sun et al. 2014 (link)). Proteins were probed with the following antibodies: SIAH1 rabbit pAb (Abcam, Cambridge, MA, USA), Phospho-p53 (Ser15) rabbit pAb (Cell Signaling, Beverly, MA, USA), p53 mouse mAb (Abcam, Cambridge, MA, USA), β-Actin mouse mAb (Santa Cruz, SantaCruz, CA, USA), Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (Cell Signaling, Beverly, MA, USA), p38 MAPK rabbit pAb (Cell Signaling, Beverly, MA, USA), Phospho-MAPKAPK-2 (Thr222) antibody (Cell Signaling, Beverly, MA, USA), MAPKAPK-2 antibody (Cell Signaling, Beverly, MA, USA), PUMA rabbit pAb (Abcam, Cambridge, MA, USA), Bak rabbit pAb (Cell Signaling, Beverly, MA, USA) and Cleaved Caspase-3 (Asp175) rabbit pAb (Cell Signaling, Beverly, MA, USA). The membranes were developed on a Kodak X-OMAT 2000A imaging system (Kodak, Rochester, NY, USA). The intensity of the protein band was analyzed by ImageJ software (1.46b, National Institutes of Health, USA). All Western blot analyses were performed in triplicate.

Yuan F., Chen X., Liu J., Feng W., Wu X, & Chen S.Y. (2016). Up-regulation of Siah1 by ethanol triggers apoptosis in neural crest cells through p38 MAPK-mediated activation of p53 signaling pathway. Archives of toxicology, 91(2), 775-784.

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Western Blotting Analysis of Apoptosis-Related Proteins

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Western blotting was performed by standard protocols as described previously (Chen et al., 2013b (link); Sun et al., 2014 (link)). Proteins were probed with the following antibodies: SIAH1 rabbit pAb (Abcam, Cambridge, MA, United States), β-Actin mouse mAb (Santa Cruz, Santa Cruz, CA, United States), Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (Cell Signaling Technology, Inc., Beverly, MA, United States), p38 MAPK rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States), Phospho-p53 (Ser15) rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States), p53 mouse mAb (Abcam, Cambridge, MA, United States), p53 upregulated modulator of apoptosis (PUMA) rabbit pAb (Abcam, Cambridge, MA, United States), Bcl-2 homologous antagonist/killer (Bak) rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States), and cleaved caspase-3 (Asp175) rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States). The membranes were developed on Molecular Imager ChemiDoc XRS + (Bio-Rad, Hercules, CA, United States), and the intensity of the protein band was analyzed by ImageJ software (1.48V, National Institutes of Health, United States). All Western blot analyses were performed in triplicate.

Yuan F., Yun Y., Fan H., Li Y., Lu L., Liu J., Feng W, & Chen S.Y. (2020). MicroRNA-135a Protects Against Ethanol-Induced Apoptosis in Neural Crest Cells and Craniofacial Defects in Zebrafish by Modulating the Siah1/p38/p53 Pathway. Frontiers in Cell and Developmental Biology, 8, 583959.

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