Iraffinity 1 ftir spectrometer

After overnight drying of the OSA-COS samples in a 70 °C oven, 1 mg of material was ground with 100 mg of KBr to a uniform powder, as described in our previous publication [28 (link)]. A manual press and pellet die were used to obtain a thin pellet. The samples were analyzed on an IR Affinity-1 FTIR spectrometer (Shimadzu, Nagoya, Japan) between 4000 and 400 cm⁻¹. Sixty-four scans were performed at a 4.0 cm⁻¹ resolution.

Melting points were determined in the open capillary tubes on an Electrothermal IA 9300 melting point apparatus Electrothermal Engineering Ltd, Rochford, UK. The values given are uncorrected. Optical rotation properties were measured with an ATAGO SAC-I polarimeter (Atago, Tokyo, Japan) using a sodium lamp (589 nm) at 20 °C. The NMR spectra (600/150 MHz) were recorded on a Bruker Avance (Bruker , Billerica, MA, USA) III 600 spectrometer in CDCl₃. Chemical shifts were reported in ppm (δ), and J values in Hz. Multiplicity was designated as the singlet (s), doublet (d), triplet (t), quartet (q) and multiplet (m). High-resolution mass spectral (HR-MS) analysis was performed on a Bruker Impact II instrument. Solid state infrared spectra were recorded in the range of 4000–1000 cm⁻¹ using the Shimadzu IRAffinity-1 FTIR spectrometer (Shimadzu, Kyoto, Japan) and KBr pellet method. Thin layer chromatography (TLC) was performed on silica gel 60 254F plates (Merck, Darmstadt, Germany) using a mixture of different organic solvents as an eluent. The chromatographic spots were detected by spraying with a solution of 5% sulfuric acid, followed by heating. Column chromatography was performed on silica gel 60, <63 μm (Merck), with the mixture of chloroform and ethanol (15:1, v/v) or hexane and ethyl acetate (3:2, v/v) as an eluent. Solvents were dried and purified according to usual procedures.

Fourier-transform infrared (FTIR) spectroscopy was used to study the interaction between lipopolymers and amino acids as well as the changes induced by the polymerization process. To this end, 0.4 ml of the liposomal suspension with or without amino acids or polymerization was lyophilized. The solid was then resuspended in D₂O in order to avoid interference induced by H₂O. The lipid suspension was pipetted onto ATR cells and dried with a hair drier. Infrared spectra were recorded from 3000 to 1000 cm⁻¹ in an IRAffinity-1 FTIR spectrometer (Shimadzu). Data were analyzed, normalized, and baseline corrected with the IRsolution software (Shimadzu). Frequencies were determined by the peak identification routine of the software.

The FT-IR spectra of the unmodified and modified agar samples were obtained using the method described by Gope et al. [37 (link)] with an IRAffinity-1 FT-IR spectrometer (Shimadzu, Kyoto, Japan).

Fourier transform infrared spectroscopy (FTIR) was performed using a Shimadzu IRAffinity‐1 FTIR spectrometer. The oil samples were applied to the surface of KBr pellets. After 24 hr of vacuum drying, the FTIR spectra of oil samples were recorded in the region of 4,000–1,660 cm⁻¹, with 120 scans acquired at a resolution of 4 cm⁻¹.

The metal ion (Cu/Ni) content in the Cu@Ni-MOF was recorded by inductively coupled plasma mass spectrometry (ICP–MS) (Icap Qc). The electrochemical measurements were carried out on a CHI 660E potentiostat electrochemical workstation (Thermo-Fisher, Berlin, Germany). X-ray diffraction (XRD) patterns were recorded with a Rigaku Miniflex 600 X-ray diffractometer (Rigaku, Tokyo, Japan) from 5° to 50°. An infrared spectrum was recorded using an IR Affinity-1 FT-IR spectrometer (Shimadzu, Kyoto, Japan) in the range of 400–4000 cm⁻¹. Cu(NO₃)₂, Ni(NO₃)₂, 2,6-pyridinedicarboxylic acid were bought commercially(Jinan Henghua Technology Co., Ltd., Jinan, China), acetylene carbon black and PolyVinylidene Fluoride were bought commercially(TIMCAL, Changzhou, China)