The cells were harvested and stained with Tetrechloro-tetraethyl benzimidazol carbocyanine iodide 1 (JC-1) for 15 min at room temperature in the dark. Then the Δψm was assessed using JC-1 Mitochondrial Potential Detection Kit (Biotium Inc, US) following the manufacture’s protocol. The fluorescence signal was measured at 550 nm and 485 nm.
Jc 1 mitochondrial potential detection kit
Manufactured by Biotium
Sourced in United States
The JC-1 Mitochondrial Potential Detection Kit is a laboratory tool used to measure the mitochondrial membrane potential in cells. It contains the fluorescent dye JC-1, which exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green to red.
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6 protocols using jc 1 mitochondrial potential detection kit
1
Mitochondrial Membrane Potential Measurement
2
Mitochondrial Membrane Potential Analysis
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The transmembrane potential ΔΨm was analyzed using a JC-1 Mitochondrial Potential Detection Kit (Biotium Inc.) by FCM. The cell suspension was filtered through a 300-mesh nylon mesh, washed twice with cold PBS, and stained by 5,5′,6,6′-tetrachloro-1,1′,3,3′ tetraethylbenzimidazolcarbocyanine iodide (JC-1; Molecular Probes) in PBS for 15 min at room temperature in the dark, followed by flow cytometric analysis.
3
Measuring Mitochondrial Membrane Potential
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The transmembrane potential, ΔΨm, was analyzed using the JC-1 Mitochondrial Potential Detection Kit (Biotium Inc., Hayward, CA, USA). The cell suspension was filtered through 300-mesh nylon, washed twice with cold PBS and stained with 5,5′,6,6′-tetrachloro-1,1′,3,3′ tetraethylbenzimidazolcarbocyanine iodide (JC-1; Molecular Probes) in PBS for 15 min at room temperature in the dark, followed by flow cytometric analysis.
4
Analyzing Mitochondrial Potential Dynamics
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The mitochondrial transmembrane potential Δψm was analyzed using a JC-1 Mitochondrial Potential Detection Kit (Biotium Inc., Hayward, CA, US). Briefly, HeLa cells (3 × 105 per well) were seeded into 30 mm dishes with 1.8 mL complete culture medium and exposed to NTP for 10, 20 or 40 s. After 24 h incubation, cells were harvested and stained by JC-1 in PBS for 30 min at room temperature in the dark, followed by flow cytometric analysis.
5
Mitochondrial Potential Analysis via JC-1
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The transmembrane potential, ΔΨm was analyzed using a JC-1 Mitochondrial Potential Detection Kit (Biotium Inc., Hayward, CA, USA). The cell suspension was filtered through a 300-mesh nylon mesh, washed twice with cold PBS and stained by 5,5′,6,6′-tetrachloro-1,1′,3,3′ tetraethylbenzimidazolcarbocyanine iodide (JC-1; Molecular Probes, Eugene, OR, USA) in PBS for 15 min at room temperature in the dark, followed by flow cytometric analysis.
6
Mitochondrial membrane potential analysis
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The transmembrane potential ΔΨm was analysed using a 5,5′,6,6′-tetrachloro-1,1′,3,3′ tetraethylbenzi midazolcarbocyanine iodide (JC-1) Mitochondrial Potential Detection Kit (Biotium Inc., Hayward, CA, US). The cell suspension was filtered through a 300-mesh nylon mesh, washed twice with cold PBS and stained by JC-1in PBS for 15 min at room temperature in the dark, followed by flow cytometric analysis.
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