Celltiter luminescent cell viability assay

Manufactured by Promega

Sourced in United States

The CellTiter Luminescent Cell Viability Assay is a laboratory product designed to measure the number of viable cells in a sample. It is a luminescent-based assay that quantifies the amount of ATP present, which is an indicator of metabolically active cells. The assay provides a straightforward method for determining the relative number of living cells in a population.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Infinite f200 pro microplate reader, by Tecan (1 mentions) Caspase 3 7 kit, by Promega (1 mentions) Caspase glo 3 7 assay, by Promega (1 mentions) Dtx 880 multimode detector, by Beckman Coulter (1 mentions) Cytation 3 microplate reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

CellTiter-Glo Luminescent Cell Viability Assays kit Luminescent assays

6 protocols using celltiter luminescent cell viability assay

Measuring Inhibitor Cytotoxicity via Luminescent Assay

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To determine inhibitor toxicity, Promega’s CellTiter Luminescent Cell Viability Assay (G7571) was used to measure cellular viability 24 hours after inhibitor treatment, as described previously [18 (link)].

Lundberg L., Pinkham C., de la Fuente C., Brahms A., Shafagati N., Wagstaff K.M., Jans D.A., Tamir S, & Kehn-Hall K. (2016). Selective Inhibitor of Nuclear Export (SINE) Compounds Alter New World Alphavirus Capsid Localization and Reduce Viral Replication in Mammalian Cells. PLoS Neglected Tropical Diseases, 10(11), e0005122.

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Cell Viability Assay Using ATP Measurement

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Cell viability based on ATP measurement was assessed using CellTiter Luminescent Cell Viability Assay (Promega). Briefly, NSCLCs cells were seeded in a 96-well plate at a density of 2 × 10⁴/well and treated with gradient concentration of 17-AAG. After incubation, 100 μL staining solution (CellTiter-Glo reagent) was added to each well and mixed for 2 min on an orbital shaker to induce cell lysis. Cells were incubated at room temperature for 10 min to stabilize the luminescence signal, which was recorded using the microplate reader. The experiment was run in triplicate. The plate was incubated for 10 min and the luminescent signal was recorded using Infinite^® F200 pro microplate reader (Tecan).

Wang J., Lu Q., Cai J., Wang Y., Lai X., Qiu Y., Huang Y., Ke Q., Zhang Y., Guan Y., Wu H., Wang Y., Liu X., Shi Y., Zhang K., Wang M, & Peng Xiang A. (2019). Nestin regulates cellular redox homeostasis in lung cancer through the Keap1–Nrf2 feedback loop. Nature Communications, 10, 5043.

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Cultivating PC12 Cells for Viability Assays

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PC12 cell line was obtained from the Cell Line Resource Center of the Chinese Academy of Sciences (Shanghai, China). PC12 cells stably transfected with APP695 Swedish mutation were cultured in Dulbecco's modified Eagle's medium (DMEM, ThermoFisher) with 10% FBS (ThermoFisher) and 1×penicillin‐streptomycin. Cell cultures were maintained in a humidified atmosphere of 5% CO₂ at 37°C. Cell viability was measured using Cell Titer Luminescent Cell Viability Assay (Promega, USA) in 96‐well plates in accordance with manufacturer instructions.

Hu Y., Zhang Y., Ren R., Dammer E.B., Xie X., Chen S., Huang Q., Huang W., Zhang R., Chen H., Wang H, & Wang G. (2021). microRNA‐425 loss mediates amyloid plaque microenvironment heterogeneity and promotes neurodegenerative pathologies. Aging Cell, 20(10), e13454.

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Cell Viability, Proliferation, and Apoptosis Assays

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Cell viability was assayed using either the CellTiter AQueous Non-Radioactive Cell Proliferation Assay (MTS, Huntsville, AL, USA) or CellTiter Luminescent Cell Viability Assay (Promega, Madison, WI, USA) as described previously.^{18 (link)} BrdU incorporation was assayed using a BrdU Cell Proliferation Assay according to the manufacturer's suggested protocol (Calbiochem, Billerica, MA, USA). Real time analysis of cellular proliferation by xCELLligence assay was performed as described previously.^{18 (link)}Apoptosis was determined by nuclear fragmentation assay as described previously.^{54 (link), 55 (link), 56 (link), 57 (link)} Quantifications were performed in triplicate, with each count consisting of at least 300 cells. Caspase 3/7 activation was evaluated by Caspase 3/7 kit (Promega) according to the manufacturer's suggested protocol.

O'Donnell E.F., Koch D.C., Bisson W.H., Jang H.S, & Kolluri S.K. (2014). The aryl hydrocarbon receptor mediates raloxifene-induced apoptosis in estrogen receptor-negative hepatoma and breast cancer cells. Cell Death & Disease, 5(1), e1038-.

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Measuring Cell Viability and Caspase Activation in Wild-type and EGR1 Knockout MEFs

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Wild-type and EGR1^−/− mouse embryonic fibroblasts (MEFs) were infected with TrD at various MOIs for an hour and then washed with PBS, and the medium was replaced. Cell viability was measured at 24 h postinfection using a Promega CellTiter luminescent cell viability assay (catalog number G7571) according to the manufacturer's protocol. Luminescence was read using a Beckman Coulter DTX 880 multimode detector with an integration time of 100 ms per well. Similarly, caspase activation in infected wild-type and EGR1^−/− MEFs was measured at 24 h postinfection using a Promega Caspase 3/7 Glo assay (catalog number G8090) according to the manufacturer's protocol. Luminescence was read using the DTX 880 multimode detector with an integration time of 100 ms per well.

Baer A., Lundberg L., Swales D., Waybright N., Pinkham C., Dinman J.D., Jacobs J.L, & Kehn-Hall K. (2016). Venezuelan Equine Encephalitis Virus Induces Apoptosis through the Unfolded Protein Response Activation of EGR1. Journal of Virology, 90(7), 3558-3572.

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Luminescent Cell Viability Assay

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Cell viability based on ATP measurement was assessed using CellTiter Luminescent Cell Viability Assay (Promega, Madison, WI, USA). Briefly, cells were seeded in a 96-well plate and, at the day of the assay, the medium was removed from the wells and replaced by 50 μl of fresh medium. 50 μl of the Cell Titer-Glo® reagent was added and mixed for 2 min on an orbital shaker to promote cell lysis. The plate was incubated for 10 min and the luminescent signal was recorded using Cytation™ 3 microplate reader (BioTek, USA).

Cunha-Oliveira T., Ferreira L.L., Coelho A.R., Deus C.M, & Oliveira P.J. (2018). Doxorubicin triggers bioenergetic failure and p53 activation in mouse stem cell-derived cardiomyocytes. Toxicology and applied pharmacology, 348.

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