Anti phospho tyr701 stat 1

Manufactured by Cell Signaling Technology

Anti-phospho-(Tyr701) STAT 1 is a laboratory reagent used to detect the phosphorylation of signal transducer and activator of transcription 1 (STAT1) on tyrosine 701. This antibody is designed for use in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the activation and signaling of STAT1.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti stat1, by Cell Signaling Technology (1 mentions) Anti stat4, by Cell Signaling Technology (1 mentions) Rabbit anti stat6, by Abcam (1 mentions) Anti gata3 hg3 31, by Santa Cruz Biotechnology (1 mentions) Protease phosphatase inhibitor cocktail, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti histone h3, by Abcam (1 mentions) Anti acetyl histone h3, by Merck Group (1 mentions) Anti histone h1, by Abcam (1 mentions) Ab4086, by Abcam (1 mentions) Anti pol 2, by Santa Cruz Biotechnology (1 mentions) Anti irf9, by Santa Cruz Biotechnology (1 mentions) Anti irf1, by Santa Cruz Biotechnology (1 mentions) Chemidoc analyzer, by Bio-Rad (1 mentions) Anti pan erk, by BD (1 mentions) Anti stat1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Phospho-STAT (4 citations) STAT-1 (Tyr701) (2 citations)

3 protocols using anti phospho tyr701 stat 1

Immunoblotting Analysis of Murine Naïve T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse naïve T cells stimulated in vitro as indicated above were lysed in the presence of protease/phosphatase inhibitor cocktail (catalog no. 5872; Cell Signaling Technology, Danvers, MA) for 30 min on ice. The lysates were centrifuged at 12000 × g for 30 min, 4 °C and the supernatants were subjected to standard SDS-PAGE followed by transferring to PVDF membranes for detection of STAT, T-bet or GATA-3 proteins. Rabbit anti-STAT1 (catalog no. 9172S), anti-phospho-(Tyr701) STAT 1 (catalog no. 9167S), anti-STAT4 (catalog no. 2653S), and anti-phospho-(Tyr693) STAT 4 (catalog no. 4134S) were from Cell Signaling Technology. Rabbit anti-STAT6 (catalog no. ab32520) and anti-phospho-(Tyr641) STAT 6 (catalog no. ab54461) were from Abcam. Anti-T-bet mAb 4B10 (catalog no. sc-21749) and anti-GATA-3 HG3-31 (catalog no. sc-268) were from Santa Cruz (Dallas, TX, USA).

Zhang L., Liu S.H., Wright T.T., Shen Z.Y., Li H.Y., Zhu W., Potempa L.A., Ji S.R., Szalai A.J, & Wu Y. (2015). C-reactive protein directly suppresses T helper 1 cell differentiation and alleviates experimental autoimmune encephalomyelitis. Journal of immunology (Baltimore, Md. : 1950), 194(11), 5243-5252.

+ Open protocol

+ Expand

Cell Culture and Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa S3 and HEK293 cells were grown in Dulbecco's modified essential medium supplemented with 10% fetal calf serum. Antibodies used in this study are as follows: Anti-STAT1α/β (sc-346; Santa Cruz Biotechnology), anti-STAT2 (sc-476; Santa Cruz Biotechnology), anti-IRF9 (sc-10793; Santa Cruz Biotechnology), anti-phospho-(Tyr701) STAT1 (#9171; Cell Signaling Technology), anti-phospho-(Tyr689) STAT2 (#07-224; Upstate Biotechnology), anti-phospho-(Ser727) STAT1 (#06-802; Upstate Biotechnology), anti-Pol II (sc-899; Santa Cruz Biotechnology), anti-β-Actin (A5441; SIGMA), anti-Histone H3 (ab1791; Abcam), anti-acetyl-Histone H3 (06-599; Millipore), anti-Histone H1.2 (ab4086; Abcam), anti-Flag (F3165; SIGMA) and anti-TAF-Iα/β (monoclonal antibody KM1725) (34 (link)) antibodies.

Kadota S, & Nagata K. (2014). Silencing of IFN-stimulated gene transcription is regulated by histone H1 and its chaperone TAF-I. Nucleic Acids Research, 42(12), 7642-7653.

+ Open protocol

+ Expand

IFNγ-Induced Protein Analysis in BMDMs

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMDMs (10⁶ cells/well) were stimulated with IFNγ (100 U/ml) for the times indicated, lysed and used for Western blot analysis as described previously (30 (link)) with the following adaptations: cells were lysed in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% IGEPAL CA-630 (v/v), 10% glycerol (v/v), 0.1 mM EDTA, 2 mM DTT, 0.2 mM Na-vanadate, 25 mM Na-fluoride, 1 μg/ml leupeptin, 1 μg/ml aprotinin, 0.1 μg pepstatin and 1 mM PMSF. The following antibodies were used: anti-IRF1 (Santa Cruz, SC-640), anti-phospho-Tyr701 STAT1, and anti-STAT1 (Cell Signaling Technology, 9167 and 9172), anti-pan-ERK (BD Transduction Laboratories, 610123; p42 is shown in our experiments). Peroxidase-conjugated secondary antibodies (mouse and rabbit) were from Cell Signaling Technology (7076 and 7074). Blots were scanned with a Chemidoc analyzer (Bio-Rad).

Parrini M., Meissl K., Ola M.J., Lederer T., Puga A., Wienerroither S., Kovarik P., Decker T., Müller M, & Strobl B. (2018). The C-Terminal Transactivation Domain of STAT1 Has a Gene-Specific Role in Transactivation and Cofactor Recruitment. Frontiers in Immunology, 9, 2879.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!