Puc lacz

Manufactured by Bloomington Drosophila Stock Center

Sourced in United Kingdom

Puc-LacZ is a genetic reporter construct that contains the lacZ gene encoding the enzyme β-galactosidase under the control of the Puc (Puckered) promoter. The Puc gene is involved in the Jun N-terminal kinase (JNK) signaling pathway, which regulates various cellular processes. The Puc-LacZ construct is commonly used to monitor the expression of the Puc gene and the activity of the JNK pathway in Drosophila, a model organism for genetic and developmental studies.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using puc lacz

Generating Mutant Clones in Drosophila Wing Disc

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following stocks were used: UAS-Ras^V12 (Lee et al., 1996 (link)); nub-Gal4 (Calleja et al., 1996 (link)); hid 5′F-GFP (Tanaka-Matakatsu et al., 2009 (link)); UAS-DER^DN, UAS-Diap1, UAS-puc, ap-Gal4, puc-LacZ, rpr-LacZ, and brk-LacZ (Bloomington Drosophila Stock Center); mys^RNAi, UAS-diβ (Martin-Bermudo and Brown, 1999 (link)) and hid^RNAi (Vienna Drosophila RNA-i Center). To generate mutant clones in the wing disc we used the FRT/FLP technique (Chou and Perrimon, 1992 (link)). Mutant clones were marked by the absence of GFP. The following mutant alleles and chromosomes were used: mys¹¹ [also known as mys^XG43 (Bunch et al., 1992 (link))] and e22c-Gal4 UAS-flipase (Duffy et al., 1998 (link)). The e22c-Gal4 driver is expressed in some posterior wing disc cells and was therefore used in combination with UAS- flp to generate large mys clones. Flies were raised at 25°C.

Valencia-Expósito A., Gómez-Lamarca M.J., Widmann T.J, & Martín-Bermudo M.D. (2022). Integrins Cooperate With the EGFR/Ras Pathway to Preserve Epithelia Survival and Architecture in Development and Oncogenesis. Frontiers in Cell and Developmental Biology, 10, 892691.

+ Open protocol

+ Expand

Drosophila Genetic Stocks for Functional Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following stocks were obtained from the Bloomington Stock Center: Oregon R, w¹¹¹⁸, msn-lacZ (msn⁰⁶⁹⁴⁶), puc-lacZ, UAS-Jra, UAS-hep, e16E-GAL4, hs-GAL4, UAS-cdc37-RNAi (JF03184, GD14633, HMS01401), UAS-bsk^DN, UAS-luciferase RNAi, UAS-GFP.nls, UAS-Dcr-2, UAS-cdk2-RNAi (HMS00174; Sopko et al., 2014 ), UAS-cycE-RNAi (HMS00060), and UAS-ckIIα^DN (Lin et al., 2002) . The following stocks were obtained from the Vienna Drosophila RNAi Center in Austria: UAS-cdc37-RNAi (KK102575), UAS-aurora B-RNAi (GD11982, KK112558; Bell et al., 2015 (link)), and UAS-cdc2-RNAi (106130). UAS-cdc37-RNAi (12019R-2) was obtained from the National Institute of Genetics in Japan. D. mojavensis was obtained from The National Drosophila Species Stock Center.

Lee C.W., Kwon Y.C., Lee Y., Park M.Y, & Choe K.M. (2019). cdc37 is essential for JNK pathway activation and wound closure in Drosophila. Molecular Biology of the Cell, 30(21), 2651-2658.

+ Open protocol

+ Expand

Drosophila Transgene Expression Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drosophila melanogaster flies were raised on standard media; stocks were kept at room temperature and crosses were reared at 25°C or 29°C as indicated. Four Gal4 fly lines were used to induce transgene expression: dpp-Gal4, UAS-GFP/TM6B (Swarup et al., 2015 (link)), Dll-lacZ/Cyo; Hh-Gal4/TM6B (Hall et al., 2017 (link)), tj-Gal4/tj-Gal4 (a gift from Dr Nicholas Harden, Simon Fraser University; Vlachos et al., 2015 (link)), and apterous (ap)-Gal4 (Bloomington Drosophila Stock Center, 3041). Additional stocks used were: puc-LacZ (Martin-Blanco et al., 1998 (link)) and UAS-dsh (Bloomington Drosophila Stock Center, 9453). As controls, the Gal4 drivers were crossed with w¹¹¹⁸ or UAS-GFP flies. To generate transgenic UAS stock lines, patient variant Flag-tagged DVL1 constructs were sent for integration into the attP40 locus on the second chromosome for generation of stably integrated fly strains (BestGene, CA, USA).

Gignac S.J., MacCharles K.R., Fu K., Bonaparte K., Akarsu G., Barrett T.W., Verheyen E.M, & Richman J.M. (2023). Mechanistic studies in Drosophila and chicken give new insights into functions of DVL1 in dominant Robinow syndrome. Disease Models & Mechanisms, 16(4), dmm049844.

+ Open protocol

+ Expand

Drosophila Genetic Interaction Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drosophila were raised on standard cornmeal–yeast–sucrose medium at 25°C on a 12 h light:dark cycle. CHMP2B^Intron5 flies were described previously (7 (link),10 (link)). All other stocks were obtained from the following sources: POSH⁷⁴ (Toshiro Aigaki, Tokyo Metropolitan University, Japan) (29 (link)), AKT¹ (Clive Wilson, University of Oxford, UK) (25 (link)), OK6-Gal4 (Cahir O’Kane, University of Cambridge, UK), UAS-myrAKT, UAS-mCD8-GFP, AKT⁰⁴²²⁶, AKT³, UAS-AKT-RNAi (BL #33615), UAS-mCherry-POSH, UAS-POSH-RNAi (BL #64569), Df(3L)H99, Puc-LacZ, glass multimer reporter (GMR)-Gal4, nSyb-Gal4, Canton S, w¹¹¹⁸ (Bloomington Stock Center). UAS-AKT (FlyORF, Zurich, Switzerland). All wild-types were an outcross of Canton S to w¹¹¹⁸.
Genetic interaction experiments and quantification of the CHMP2B^Intron5 eye phenotype was performed as described previously (7 (link)). Eyes were imaged using an AxioCam ERc 5s camera (Carl Zeiss) mounted on a Stemi 2000-C stereo microscope (Carl Zeiss).

West R.J., Ugbode C., Gao F.B, & Sweeney S.T. (2018). The pro-apoptotic JNK scaffold POSH/SH3RF1 mediates CHMP2BIntron5-associated toxicity in animal models of frontotemporal dementia. Human Molecular Genetics, 27(8), 1382-1395.

+ Open protocol

+ Expand

Genetic Toolkit for Drosophila Development

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following transgenes were used:
ap-Gal4 (Bloomington #3041),
dpp-Gal4 (Milán and Cohen, 1999),
pnr-Gal4 (Bloomington #3039),
UAS-eyg (Bloomington #26809),
UAS-pcp (Bloominton #15367),
UAS-pnr (Bloomington #7223),
UAS-sdr (Kyoto Stock Center #206122),
UAS-tkv^DN (a gift from C. Dahmann),
UAS-arpc3B-RNAi (VDRC #105278),
UAS-attc-RNAi (VDRC #101213),
UAS-βTub56D-RNAi (VDRC #109736),
UAS-βTub97EF-RNAi (VDRC #105075),
UAS-CG12164-RNAi (Tsinghua Fly Center #THU0252),
UAS-CG14456-RNAi (VDRC #102147),
UAS-Doc1-RNAi (VDRC #104927),
UAS-Doc2-RNAi (VDRC #37634),
UAS-Doc3-RNAi (VDRC #30550),
UAS-mec2-RNAi (VDRC #104601),
UAS-pnr-RNAi (VDRC #6224),
puc-lacZ (P{lArB}puc[A251.1F3], Bloomington #11173).
Larvae were raised at 25 °C unless stated otherwise. Larvae of RNAi transgenes were raised at 29 °C for efficient expression. The genotype of all crosses is listed in the Supplementary Materials.

Lu J., Wang Y., Wang X., Wang D., Pflugfelder G.O, & Shen J. (2022). The Tbx6 Transcription Factor Dorsocross Mediates Dpp Signaling to Regulate Drosophila Thorax Closure. International Journal of Molecular Sciences, 23(9), 4543.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!