Rapidpoint 500 analyzer

All measurements were taken at baseline without phrenic nerve stimulation with the intact phrenic nerve and at the different stimulation levels still with the phrenic nerve being intact. Thereafter, the phrenic nerve was transected, and the same varying stimulation levels were applied. Diaphragmatic movement was recorded by fluoroscopy (Ziehm Vista, Ziehm, Nuremberg, Germany) and evaluated with a tangent drawn at the highest point of the right hemi-diaphragm. At this point, diaphragmatic movement was measured at a right angle in millimeters. Arterial blood gas analyses were conducted in a RapidPoint® 500 analyzer (Siemens Healthineers, Erlangen, Germany). Ventilation parameters were measured using Servo-i (Maquet, Rastatt, Germany), and baseline ventilation settings were an inspiratory pressure of 15 cmH₂O, a positive end-expiratory pressure of 5 cmH₂O, and a ventilation rate of 15/min.

Blood gas analysis was performed using the RapidPoint 500 analyzer (Siemens, Germany). Complete blood cell count was measured by Sysmex XN-9000 (Sysmex, Germany). Prothrombin Time (PT) and aPTT were measured by Sysmex CS-5100 using Innovin and Actin FSL reagents (Siemens, Germany), respectively. C-Reactive Protein (CRP) and liver parameters (ALAT, ASAT, GGT) were measured by Architect C16000 (Abbott, USA). Ferritin was measured by Architect i2000SR (Abbott, USA).