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Quick load 2 master mix

Manufactured by New England Biolabs
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Quick-Load 2× master mix is a pre-mixed solution containing reagents necessary for PCR amplification. It includes Taq DNA polymerase, reaction buffer, MgCl2, and dNTPs in a ready-to-use format.

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Lab products found in correlation

Standard buffer, by New England Biolabs (2 mentions) Sanger sequencing, by Eurofins (2 mentions)

Spelling variants of this product

OneTaq Quick-Load 2 Master Mix (8 citations) Quick-Load® Taq 2 × Master Mix (7 citations) OneTaq Quick-Load 2× Master Mix with Standard Buffer (3 citations) OneTaq Hot Start Quick-Load 2× Master Mix (2 citations)

2 protocols using quick load 2 master mix

1

Genomic Island Mobilization Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine whether a genomic island is mobilizable, we designed primers spanning the excision site and primers targeting the key genes present in the genomic island (Table S2). The PCR reaction was conducted using Quick-Load 2× master mix with standard buffer (New England Biolabs, Ipswich, MA, USA) and a 200 nM concentration of each primer. The genomic island product was further analyzed by Sanger sequencing (Eurofins Genomics, Germany).
Kovařovic V., Finstrlová A., Sedláček I., Petráš P., Švec P., Mašlaňová I., Neumann-Schaal M., Šedo O., Botka T., Staňková E., Doškař J, & Pantůček R. (2023). Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex. Microbiology Spectrum, 11(5), e01342-23.
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2

Genomic Island Mobilization Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine whether a genomic island is mobilizable, we designed primers spanning the excision site and primers targeting the key genes present in the genomic island (Table S2). The PCR reaction was conducted using Quick-Load 2× master mix with standard buffer (New England Biolabs, Ipswich, MA, USA) and a 200 nM concentration of each primer. The genomic island product was further analyzed by Sanger sequencing (Eurofins Genomics, Germany).
Kovařovic V., Finstrlová A., Sedláček I., Petráš P., Švec P., Mašlaňová I., Neumann-Schaal M., Šedo O., Botka T., Staňková E., Doškař J, & Pantůček R. (2023). Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex. Microbiology Spectrum, 11(5), e01342-23.
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