Ab209780

Manufactured by Abcam

Sourced in United Kingdom

Ab209780 is a recombinant protein used in research applications. It is produced in a mammalian expression system.

Automatically generated - may contain errors

Lab products found in correlation

Irdye 800cw donkey anti rabbit, by LI COR (2 mentions) Pa1 26204, by Thermo Fisher Scientific (2 mentions) Ma5 15122, by Thermo Fisher Scientific (2 mentions) Irdye 680rd donkey anti mouse secondary antibodies, by LI COR (2 mentions) Ma191399, by Thermo Fisher Scientific (2 mentions) Polyvinylidene difluoride, by Merck Group (1 mentions) Ab205270, by Abcam (1 mentions) Ripa buffer, by Beyotime (1 mentions) Ab8245, by Abcam (1 mentions)

3 protocols using ab209780

Immunofluorescence analysis of ECM proteins

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Primary antibodies used in this study were: recombinant rabbit anti-cellular communication network factor 2 (CCN2) monoclonal antibody diluted to a concentration of 1:1,000 (Abcam, ab209780); rabbit anti-bone morphogenetic protein 1 (BMP1) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; PA5-103,660; RRID: AB_2852994); rabbit anti-collagen type I (COL1) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; PA1-26204; RRID: AB_2260734); rabbit anti-suppressors of mothers against decapentaplegic homolog 2 (SMAD2) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; 51–1300; RRID: AB_2533896); rabbit anti-phosphorylated SMAD2 (pSMAD2) monoclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; MA5-15122; RRID: AB_10978317); and mouse anti-beta actin (β-actin) monoclonal antibody diluted to a concentration of 1:5,000 (Thermo Fisher Scientific; MA1-91399; RRID: AB_2273656).
For near-infrared fluorescence detection, IRDye® 800CW donkey anti-rabbit and IRDye® 680RD donkey anti-mouse secondary antibodies (LI-COR, 926-32213 and 926-68072, respectively) were used.

Patricelli C., Lehmann P., Oxford J.T, & Pu X. (2023). Doxorubicin-induced modulation of TGF-β signaling cascade in mouse fibroblasts: insights into cardiotoxicity mechanisms. Scientific Reports, 13, 18944.

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Quantification of Extracellular Matrix Proteins

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Primary antibodies used in this study were: recombinant rabbit anti-cellular communication network factor 2 (CCN2) monoclonal antibody diluted to a concentration of 1:1,000 (Abcam, ab209780); rabbit anti-bone morphogenetic protein 1 (BMP1) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; PA5-103660; RRID: AB_2852994); rabbit anti-collagen type I (COL1) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; PA1-26204; RRID: AB_2260734); rabbit anti-suppressors of mothers against decapentaplegic homolog 2 (SMAD2) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; 51-1300; RRID: AB_2533896); rabbit anti-phosphorylated SMAD2 (pSMAD2) monoclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; MA5-15122; RRID: AB_10978317); and mouse anti-beta actin (β-actin) monoclonal antibody diluted to a concentration of 1:5,000 (Thermo Fisher Scientific; MA1-91399; RRID: AB_2273656).
For near-infrared fluorescence detection, IRDye^® 800CW donkey anti-rabbit and IRDye^® 680RD donkey anti-mouse secondary antibodies (LI-COR, 926-32213 and 926-68072, respectively) were used.

Patricelli C., Lehmann P., Oxford J.T, & Pu X. (2023). Doxorubicin-Induced Modulation of TGF-β Signaling Cascade in Mouse Fibroblasts: Insights into Cardiotoxicity Mechanisms. Research Square.

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Western Blot Analysis of YAP1, ANKRD1, CTGF

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Protein samples were collected from cell lysates using RIPA buffer (Beyotime, Beijing, China) when the cells reached over 80% confluence. Proteins (30 μg) were transferred onto polyvinylidene difluoride (Millipore, Boston, USA) membranes post-separation on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Afterwards, the membranes were sealed with 5% skim milk in Tris Buffered Saline with Tween 20 (TBST) for 1 hour, followed by incubation with primary antibodies or GAPDH as a loading control at 4°C overnight. Subsequently, the membranes were rinsed 4 times with TBST and then incubated with secondary antibodies (Santa Cruz Biotechnology, Dallas, USA) on a slow shaker for 1 hour, followed by washing with TBST (5 times, 5 min/wash). The membranes were stained with ECL luminous liquid (GE Healthcare, Chicago, USA). The primary antibodies include anti-YAP1 (ab205270; Abcam, Cambridge, UK), anti-ankyrin repeat domain 1 (ANKRD1, ab272894; Abcam), anti-connective tissue growth factor (CTGF, ab209780; Abcam), and anti-GAPDH (ab8245; Abcam).

Qi L., Sun B., Yang B, & Lu S. (2021). Long Noncoding-RNA Component of Mitochondrial RNA Processing Endoribonuclease Promotes Carcinogenesis in Triple-Negative Breast Cancer Cells via the Competing Endogenous RNA Mechanism. Journal of Breast Cancer, 24(5), 428-442.

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