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Anti mouse cd11b pe cy7 clone m1 70

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Anti-mouse CD11b-PE-Cy7 (clone M1/70) is a fluorescently labeled antibody used for the detection and analysis of mouse CD11b-positive cells by flow cytometry. CD11b is a cell surface integrin that is expressed on various immune cells, including monocytes, macrophages, and granulocytes. This antibody is conjugated to the PE-Cy7 fluorescent dye, which allows for the identification and quantification of CD11b-positive cells in a sample.

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Lab products found in correlation

Facsaria 3, by BD (4 mentions) Anti mouse f4 80 apc, by Thermo Fisher Scientific (2 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (2 mentions) Ebioscience fixable viability dye efluor 506, by Thermo Fisher Scientific (2 mentions) Facsdiva software 6, by BD (2 mentions) Fc receptor blocker, by Miltenyi Biotec (2 mentions) Anti mouse cd11c pe, by Thermo Fisher Scientific (2 mentions) Anti mouse cd24 fluorescein 5 isothiocyanate fitc clone m1 69, by Thermo Fisher Scientific (2 mentions) Fcr blocking reagent, by Miltenyi Biotec (2 mentions)

3 protocols using anti mouse cd11b pe cy7 clone m1 70

1

Multiparameter Flow Cytometry Panel

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Anti-mouse-CD45.1-FITC (clone A20, Ly5.1), anti-mouse-CD45.2-PE (clone104, Ly5.2), anti-mouse-Ly6G/Gr-1-PE/Cy7 (cloneRB6-8C5), anti-mouse-CD45R/B220-PerCP (cloneRA3-6B2), anti-mouse-CD11b-PE/Cy7 (cloneM1/70), and anti-mouse-CD3-APC (clone145-2C11) antibodies were obtained from eBioscience (San Diego, CA, USA). REV and ISOR were purchased from Sigma (St. Louis, MO, USA). HEY-A was kindly provided by Dr. Qi Hou from the Institute of Materia Medica at Peking Union Medical College (PUMC, Beijing, China).
Wang H., Yang Y.L., Zhang H., Yan H., Wu X.J, & Zhang C.Z. (2014). Administration of the Resveratrol Analogues Isorhapontigenin and Heyneanol-A Protects Mice Hematopoietic Cells against Irradiation Injuries. BioMed Research International, 2014, 282657.
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2

Immune Cell Profiling in Lung and Peritoneal Samples

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BAL cells were labeled for anti-mouse CD11c-phycoerythrin ((PE), clone HL3, BD Biosciences) and anti-mouse F4/80-allophycocyanin ((APC), clone BM8, BioLegend) antibodies. Peritoneal cells were labelled for anti-mouse F4/80-APC and anti-mouse CD11b-PE-Cy7 (clone M1/70, eBioscience). The FcR Blocking Reagent (Miltenyi Biotec) was used to prevent non-specific bonding of antibody conjugates. To discriminate live and dead cells, the eBioscience™ Fixable Viability Dye eFluor™ 506 (Thermo Fischer Scientific) was used based on the manufacturer’s recommendation. The CD11c-F4/80 double positive alveolar macrophages, F4/80loCD11blo small peritoneal macrophages, and F4/80hiCD11bhi large peritoneal macrophages were sorted by FACSAria™ III (BD Biosciences). Approximately 15,000–25,000 cells were separated for transcript analysis. The flow cytometry analysis and cell sorting were performed by BD FACSAria III (BD Biosciences) using BD FACSDiva Software 6.0 (BD Biosciences).
To determine total immune cell numbers of BAL samples, the CountBright™ Absolute Counting Beads (Thermo Fischer Scientific), Fc Receptor blocker (Miltenyi Biotec), anti-mouse F4/80-APC, anti-mouse CD11c-PE, and anti-mouse CD24-fluorescein-5-isothiocyanate (FITC) (clone M1/69, eBioscience) were used. The acquired flow cytometry data were analyzed with FlowJo v10.8 (BD Biosciences).
Czimmerer Z., Halasz L., Daniel B., Varga Z., Bene K., Domokos A., Hoeksema M., Shen Z., Berger W.K., Cseh T., Jambrovics K., Kolostyak Z., Fenyvesi F., Varadi J., Poliska S., Hajas G., Szatmari I., Glass C.K., Bacsi A, & Nagy L. (2022). The epigenetic state of IL-4-polarized macrophages enables inflammatory cistromic expansion and extended synergistic response to TLR ligands. Immunity, 55(11), 2006-2026.e6.
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3

Immune Cell Profiling in Lung and Peritoneal Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
BAL cells were labeled for anti-mouse CD11c-phycoerythrin ((PE), clone HL3, BD Biosciences) and anti-mouse F4/80-allophycocyanin ((APC), clone BM8, BioLegend) antibodies. Peritoneal cells were labelled for anti-mouse F4/80-APC and anti-mouse CD11b-PE-Cy7 (clone M1/70, eBioscience). The FcR Blocking Reagent (Miltenyi Biotec) was used to prevent non-specific bonding of antibody conjugates. To discriminate live and dead cells, the eBioscience™ Fixable Viability Dye eFluor™ 506 (Thermo Fischer Scientific) was used based on the manufacturer’s recommendation. The CD11c-F4/80 double positive alveolar macrophages, F4/80loCD11blo small peritoneal macrophages, and F4/80hiCD11bhi large peritoneal macrophages were sorted by FACSAria™ III (BD Biosciences). Approximately 15,000–25,000 cells were separated for transcript analysis. The flow cytometry analysis and cell sorting were performed by BD FACSAria III (BD Biosciences) using BD FACSDiva Software 6.0 (BD Biosciences).
To determine total immune cell numbers of BAL samples, the CountBright™ Absolute Counting Beads (Thermo Fischer Scientific), Fc Receptor blocker (Miltenyi Biotec), anti-mouse F4/80-APC, anti-mouse CD11c-PE, and anti-mouse CD24-fluorescein-5-isothiocyanate (FITC) (clone M1/69, eBioscience) were used. The acquired flow cytometry data were analyzed with FlowJo v10.8 (BD Biosciences).
Czimmerer Z., Halasz L., Daniel B., Varga Z., Bene K., Domokos A., Hoeksema M., Shen Z., Berger W.K., Cseh T., Jambrovics K., Kolostyak Z., Fenyvesi F., Varadi J., Poliska S., Hajas G., Szatmari I., Glass C.K., Bacsi A, & Nagy L. (2022). The epigenetic state of IL-4-polarized macrophages enables inflammatory cistromic expansion and extended synergistic response to TLR ligands. Immunity, 55(11), 2006-2026.e6.
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