The lung tissues were dehydrated and embedded in paraffin. For histological examination, 4 μm-thick sections on slides were treated with 1.4% H2O2-methanol for 30 min to block endogenous peroxidase. Then, nonspecific binding was blocked with 1.5% normal saline, and the slides were incubated with rabbit anti-p21 (1:200; #ab188224; Abcam, Cambridge, UK) and rabbit anti-p16 (1:100; #ab51243; Abcam) antibodies. The next day, the sections were incubated with ABC kit reagents (Vector Laboratories, Burlingame, CA, USA). The color reaction was developed by staining with a liquid DAB + substrate kit (Golden Bridge International Inc., Mukilteo, WA, USA). After immunohistochemical staining, the slides were counterstained with Harris’s hematoxylin for 1 min.
Abc kit reagents
Manufactured by Vector Laboratories
Sourced in United States
The ABC kit reagents are a set of laboratory chemicals and solutions developed by Vector Laboratories for use in various scientific applications. The core function of these reagents is to facilitate specific laboratory procedures, such as staining, labeling, and detection, as part of the overall ABC kit system. The detailed composition and intended uses of the individual reagents are not provided in this factual and unbiased description.
Automatically generated - may contain errors
3 protocols using abc kit reagents
1
Immunohistochemical Analysis of p21 and p16
2
Immunohistochemical Analysis of OAT Expression
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Lung tissues were dehydrated and embedded in paraffin. For histological examination, 4-µm-thick tissue sections and BALF cells on slides were treated with 1.4% H2O2–methanol for 30 min to block endogenous peroxidase. Then, nonspecific binding was blocked with 1.5% normal serum, and the slides were incubated with rabbit anti-OAT polyclonal antibodies (1:200; Abcam, Cambridge, UK, #ab137679). The next day, the sections were incubated with ABC kit reagents (Vector Laboratories, Burlingame, CA, USA). The color reaction was developed by incubation with a liquid 3,3′-diaminobenzidine positive-substrate kit (Golden Bridge International, Inc., Mukilteo, WA, USA). After immunohistochemical staining, the slides were counterstained with Harris’s hematoxylin for 1 min.
3
Immunohistochemical Analysis of FASN and His-tag
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Lung tissues were dehydrated and embedded in paraffin. For histological examination, sections 4 µm thick and bronchoalveolar lavage fluid (BALF) cells on slides were treated with 1.4% H2O2–methanol for 30 min to block endogenous peroxidase. Then, nonspecific binding was blocked with 1.5% normal serum, and slides were incubated with rabbit anti-FASN polyclonal antibodies (1:200; Abcam, Cambridge, UK), mouse anti-6X His tag monoclonal antibody (1:200; Abcam). The next day, the sections were incubated with ABC kit reagents (Vector Laboratories, Burlingame, CA, USA). The color reaction was developed by staining with a liquid 3,3′-diaminobenzidine positive-substrate kit (Golden Bridge International, Inc., Mukilteo, WA, USA). After immunohistochemical staining, the slides were counterstained with Harris’s hematoxylin for 1 min.
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