Rabbit anti bcl 2 polyclonal antibody

Total antioxidant capacity (TAC) kit (Cat. No: S0119), lipid peroxidation MDA assay kit (Cat. No: S0131), and total SOD assay kit (Cat. No: S0109) were purchased from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China). Nitric oxide (NO) kit (Cat. No: A012) was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). 8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA kit was obtained from Beijing Fangcheng Biotechnology Company (Beijing, China). Rabbit anti-Nox4 polyclonal antibody (Cat. No: NB110-58849) was obtained from Novus Biologicals (Littleton, CO, USA). Mouse anti-Cyto-C monoclonal antibody and mouse anti-p-IκBα monoclonal antibody (Cat. No: sc-8404) were from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against NF-κB-p65 (ab16502), NF-κB-pp65 (ab86299), IκBα (ab32518) were obtained from Abcam Biocompany (Cambridge, MA, USA). Rabbit anti-Bcl-2 polyclonal antibody was obtained from Cell signaling (Danvers, MA, USA). Estradiol valerate (17-beta estradiol) tablets were purchased from Bayer Chemical Company (Leverkusen, Germany). All other reagents, except specially identified, were from Sigma (St. Louis, MO, USA).