Emerin pegfp n2 588

Manufactured by Addgene

Emerin pEGFP-N2 (588) is a plasmid construct that contains the gene encoding the human emerin protein fused to the enhanced green fluorescent protein (EGFP). This plasmid can be used for the expression of the emerin-EGFP fusion protein in mammalian cells.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Pcdna3 connexin43 gfp apex2, by Addgene (1 mentions) Pdisplay bira er, by Addgene (1 mentions) Pegfp n1, by Addgene (1 mentions)

2 protocols using emerin pegfp n2 588

Engineered Connexin43 Constructs for Cellular Analysis

Cited 3 times

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The ER-retention motif RRRRISLS was subcloned into a pENTR-V5-Cx43 vector to generate Cx43^RRRRISLS. Plasmids expressing ^GFPCx43, ^GFPmCherryCx43, Cx43^Avi and BirA-NLS were generated by cloning the appropriate cDNA into a pENTR vector containing the indicated tags. Plasmids expressing Cx43^ΔNLS were generated by amplifying cDNA encoding the first 236 and last 91 amino acids of rat Cx43, followed by a second PCR to unite the fragments; and finally cloned into a pENTR V5 vector. NLS-EGFP was kindly provided by Rob Parton (Addgene #67652) [69 (link)], mEmerald-Nesprin3-C-18 from Michael Davidson (Addgene #54203), Emerin pEGFP-N2 (588) from Eric Schirmer (Addgene #61985) [70 (link)], pDESTmycYBX1 from Thomas Tuschl (Addgene #19878) [71 (link)], pEGFP-C2 RanGAP from Mary Dasso (Addgene #13378) [72 (link)], pEGFP-N1 full length Importin- β was a gift from Patrizia Lavia (Addgene #106941) [73 (link)], pcDNA3 Connexin43-GFP-APEX2 (Addgene #49385) and pDisplay-BirA-ER (Addgene #20856) from Alice Ting [74 (link),75 (link)]. Experiments were performed 24 h after transient transfection of cells with Lipofectamine 2000 (Thermo Fisher Scientific), according to the manufacturer.

Martins-Marques T., Witschas K., Ribeiro I., Zuzarte M., Catarino S., Ribeiro-Rodrigues T., Caramelo F., Aasen T., Carreira I.M., Goncalves L., Leybaert L, & Girao H. (2023). Cx43 can form functional channels at the nuclear envelope and modulate gene expression in cardiac cells. Open Biology, 13(11), 230258.

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Plasmid Cloning and Expression Protocols

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pET His6 TEV LIC cloning vector (1B) was a gift from Scott Gradia (Addgene plasmid # 29653, Addgene, Cambridge, MA). Human_TFAM_NoMTS_pET28 was a gift from David Chan (Addgene plasmid # 34705). pDONR223‐VRK1 was a gift from William Hahn & David Root (Addgene plasmid # 23496). pPET‐PKR/PPase was a gift from James Cole (Addgene plasmid # 42934). pRK793 was a gift from David Waugh (Addgene plasmid # 8827). pmTurquoise2‐LAMP1 was a gift from Pablo Rivera‐Fuentes (Addgene plasmid # 110948). pEGFP plasmid (pc3DNA) was purchased from Addgene. Emerin pEGFP‐N2 (588) was a gift from Eric Schirmer (Addgene plasmid # 61985). FITC and Cy3‐labelled plasmid DNA was obtained from Mirus Bio LLC (Madison, WI).

Burger M., Kaelin S, & Leroux J. (2022). The TFAMoplex—Conversion of the Mitochondrial Transcription Factor A into a DNA Transfection Agent. Advanced Science, 9(8), 2104987.

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