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Wild type human tfrc and gapdh cdna open reading frames

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The wild type human (h) TFRC and GAPDH cDNA open reading frames are laboratory tools used to study gene expression. TFRC encodes the transferrin receptor, and GAPDH encodes the glyceraldehyde 3-phosphate dehydrogenase enzyme. These cDNA sequences provide the coding regions for the respective proteins.

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Lab products found in correlation

Polybrene, by Merck Group (2 mentions) Pseudotyped vesicular stomatitis virus lentiviral packaging system, by Cell Biolabs (2 mentions)

Close spelling variants of this product

GAPDH (1486 citations) Human GAPDH (26 citations)

2 protocols using wild type human tfrc and gapdh cdna open reading frames

1

Transferrin Receptor Mutant Functional Assay

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Wild type human (h) TFRC and GAPDH cDNA open reading frames, cloned in the expression vector pLOC under the control of a CMV promoter, were purchased from Open Biosystems. The QuikChange site-directed mutagenesis kit (Stratagene) was used to generate TFRC p.Tyr20His mutant (Mut) in the same vector. The vector expresses the green fluorescent protein TurboGFP as a transfection marker independently through an internal ribosome entry site. The vector was packaged into lentiviral particles using pseudotyped vesicular stomatitis virus lentiviral packaging system (Cell Biolabs, Inc.). Patient and control fibroblasts were pre-treated with polybrene (0.5 μg/ml, Sigma) and infected with lentivirus at a multiplicity of infection of 10. Eighteen hours post-infection, the medium was replaced with fresh culture medium and the cells were monitored every two hours for GFP expression. The transferrin uptake assay was performed when peak GFP expression was observed (24–28 hours). Mean red fluorescence intensity of 50 GFP-positive cells from 8–10 different fields was quantified using Nikon NS2 v3.2 software.
Jabara H.H., Boyden S.E., Chou J., Ramesh N., Massaad M.J., Benson H., Bainter W., Fraulino D., Rahimov F., Sieff C., Liu Z.J., Alshemmari S.H., Al-Ramadi B.K., Al-Dhekri H., Arnaout R., Abu-Shukair M., Vatsayan A., Silver E., Ahuja S., Davies E.G., Sola-Visner M., Ohsumi T.K., Andrews N.C., Notarangelo L.D., Fleming M.D., Al-Herz W., Kunkel L.M, & Geha R.S. (2015). A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency. Nature genetics, 48(1), 74-78.
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2

Transferrin Receptor Mutant Functional Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wild type human (h) TFRC and GAPDH cDNA open reading frames, cloned in the expression vector pLOC under the control of a CMV promoter, were purchased from Open Biosystems. The QuikChange site-directed mutagenesis kit (Stratagene) was used to generate TFRC p.Tyr20His mutant (Mut) in the same vector. The vector expresses the green fluorescent protein TurboGFP as a transfection marker independently through an internal ribosome entry site. The vector was packaged into lentiviral particles using pseudotyped vesicular stomatitis virus lentiviral packaging system (Cell Biolabs, Inc.). Patient and control fibroblasts were pre-treated with polybrene (0.5 μg/ml, Sigma) and infected with lentivirus at a multiplicity of infection of 10. Eighteen hours post-infection, the medium was replaced with fresh culture medium and the cells were monitored every two hours for GFP expression. The transferrin uptake assay was performed when peak GFP expression was observed (24–28 hours). Mean red fluorescence intensity of 50 GFP-positive cells from 8–10 different fields was quantified using Nikon NS2 v3.2 software.
Jabara H.H., Boyden S.E., Chou J., Ramesh N., Massaad M.J., Benson H., Bainter W., Fraulino D., Rahimov F., Sieff C., Liu Z.J., Alshemmari S.H., Al-Ramadi B.K., Al-Dhekri H., Arnaout R., Abu-Shukair M., Vatsayan A., Silver E., Ahuja S., Davies E.G., Sola-Visner M., Ohsumi T.K., Andrews N.C., Notarangelo L.D., Fleming M.D., Al-Herz W., Kunkel L.M, & Geha R.S. (2015). A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency. Nature genetics, 48(1), 74-78.
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