Bioanalyzer with high sensitivity chips

Manufactured by Agilent Technologies

The Bioanalyzer with High sensitivity chips is a lab equipment product from Agilent Technologies. It is designed to perform automated electrophoretic analysis of biological samples, providing accurate size and concentration measurements.

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Lab products found in correlation

Qubit fluorometer, by Thermo Fisher Scientific (1 mentions) E gel ex, by Thermo Fisher Scientific (1 mentions) Zymoclean gel dna recovery kit, by Zymo Research (1 mentions) Hiseq 2500 sequencing platform, by Illumina (1 mentions) Truseq chip sample prep kit, by Illumina (1 mentions) Truseq sample prep kit v2, by Illumina (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Qiaquick gel extraction kit, by Qiagen (1 mentions)

Close spelling variants of this product

Agilent BioAnalyzer with High-Sensitivity DNA chips 2100 Bioanalyzer with High Sensitivity DNA Chips Bioanalyzer with high-sensitivity DNA chips Bioanalyzer High Sensitivity Chip High Sensitivity chip High sensitivity bioanalyzer Bioanalyzer chips Bioanalyzer

2 protocols using bioanalyzer with high sensitivity chips

Single-cell Nucleosome Sequencing

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Since each single-cell library received a unique barcode, libraries can be pooled (multiplexed) and sequenced together. Per 96-well plate, the full volume (6 μL) of the single nuclei and negative controls were pooled together with 1 μL of the ten nuclei controls. Size selection was performed on a 2 % E-gel EX (Invitrogen) to isolate the mononucleosome fragments of approximately 280 bp (range of 200–400 bp). The DNA was eluted from the gel slices using Zymoclean gel DNA recovery kit (Zymo) according to the manufacturer’s protocol. The DNA quantity and quality were assessed using Qubit fluorometer (Invitrogen) and Bioanalyzer with High sensitivity chips (Agilent), respectively. For sequencing, clusters were generated on the cBot and single-end 50 nt reads were generated using the HiSeq2500 sequencing platform (Illumina, San Diego, CA, USA). In all runs, a pool of 192 libraries was sequenced on one lane of a flow cell.

van den Bos H., Spierings D.C., Taudt A.S., Bakker B., Porubský D., Falconer E., Novoa C., Halsema N., Kazemier H.G., Hoekstra-Wakker K., Guryev V., den Dunnen W.F., Foijer F., Tatché M.C., Boddeke H.W, & Lansdorp P.M. (2016). Single-cell whole genome sequencing reveals no evidence for common aneuploidy in normal and Alzheimer’s disease neurons. Genome Biology, 17, 116.

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RNA-Seq and ChIP-Seq Library Preparation

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Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. TruSeq sample Prep Kit V2 (Illumina) was used for all the RNA-Seq libraries construction according to the manufacturer’s instructions. Protocols for performing ChIP have been previously described (Shi et al., 2013 (link)). For ChIP-Seq library construction, 50–100e06 of RN2 cells were crosslinked using 1% formaldehyde for 20 minutes at room temperature. After purifying immunoprecipitated DNA using QIAquick Gel Extraction Kit (QIAGEN), ChIP-Seq library was constructed with TruSeq ChIP Sample Prep Kit (Illumina) following the manufacturer’s instructions with the following exceptions; following adapter ligation the library was amplified with 15 cycles of PCR. Both the quantity and quality of library was determined by using Bioanalyzer with High Sensitivity chips (Agilent). The main GEO accession number for the raw and processed sequencing data reported in this paper is GSE66123.
A full description of experimental procedures can be found in the Supplemental Information.

Roe J.S., Mercan F., Rivera K., Pappin D.J, & Vakoc C.R. (2015). BET bromodomain inhibition suppresses the functional output of hematopoietic transcription factors in acute myeloid leukemia. Molecular cell, 58(6), 1028-1039.

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