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U plex mouse insulin assay

Manufactured by Mesoscale
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The U-PLEX Mouse Insulin assay is a multiplex assay designed to quantify insulin levels in mouse samples. The assay utilizes Mesoscale's proprietary U-PLEX technology to enable the simultaneous measurement of multiple analytes in a single sample. The core function of this product is to provide a reliable and efficient method for the detection and quantification of insulin in mouse biological samples.

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Lab products found in correlation

Procyte dx, by IDEXX (2 mentions) β hydroxybutyrate, by Merck Group (1 mentions)

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U-PLEX assay (31 citations) U-PLEX (19 citations)

2 protocols using u plex mouse insulin assay

1

Comprehensive Blood Biomarker Analysis in Mice

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At the end of each experiment, blood samples were collected from the mice in EDTA-coated tubes. To measure blood count, fresh blood samples were analyzed on a hematology analyzer (ProCyte Dx; IDEXX Laboratories) and centrifuged at 500g for 15 min. The collected plasma concentration was used to determine leptin (Novus Biologicals, reference no. M0B00), adiponectin (Novus Biologicals, reference no. MRP300), insulin [U-PLEX Mouse Insulin assay (Meso Scale Discovery), reference no. K1526HK], IGF-1 (Novus Biologicals, reference no. MG100), growth hormone (Ozyme, reference no. MOFI00849), β-hydroxybutyrate (Sigma-Aldrich, reference no. MAK041), and triglyceride circulating levels (Sigma-Aldrich, reference no. MAK266) using enzymatic methods from commercially available kits. NEFAs were detected with an enzymatic colorimetric assay (Fujifilm; Wako). Total cholesterol, triglycerides, and creatinine were measured by IDEXX Laboratories.
Ladraa S., Zerbib L., Bayard C., Fraissenon A., Venot Q., Morin G., Garneau A.P., Isnard P., Chapelle C., Hoguin C., Fraitag S., Duong J.P., Guibaud L., Besançon A., Kaltenbach S., Villarese P., Asnafi V., Broissand C., Goudin N., Dussiot M., Nemazanyy I., Viel T., Autret G., Cruciani-Guglielmacci C., Denom J., Bruneau J., Tavitian B., Legendre C., Dairou J., Lacorte J.M., Levy P., Pende M., Polak M, & Canaud G. (2022). PIK3CA gain-of-function mutation in adipose tissue induces metabolic reprogramming with Warburg-like effect and severe endocrine disruption. Science Advances, 8(49), eade7823.
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2

Blood Collection and Analyte Quantification

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At the end of each experiment, blood samples were collected from the mice in EDTA-coated tubes. To measure blood count, fresh blood samples were analyzed on a hematology analyzer (ProCyte Dx; IDEXX Laboratories) and centrifuged at 500 × g for 15 min. The collected plasma concentration was used to determine insulin (U-PLEX mouse insulin Assay [Meso Scale Discovery], ref# K1526HK) and IGF-1 (ref# MG100; Novus Biologicals) circulating levels using enzymatic methods from commercially available kits.
Bayard C., Segna E., Taverne M., Fraissenon A., Hennocq Q., Periou B., Zerbib L., Ladraa S., Chapelle C., Hoguin C., Kaltenbach S., Villarese P., Asnafi V., Broissand C., Nemazanyy I., Autret G., Goudin N., Legendre C., Authier F.J., Viel T., Tavitian B., Gitiaux C., Fraitag S., Duong J.P., Delcros C., Sergent B., Picard A., Dussiot M., Guibaud L., Khonsari R, & Canaud G. (2023). Hemifacial myohyperplasia is due to somatic muscular PIK3CA gain-of-function mutations and responds to pharmacological inhibition. The Journal of Experimental Medicine, 220(11), e20230926.
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