Platelet parameters including MPV, PDW, PCT (plateletcrit) and PLT (platelet count) were measured with the Mindray BC-5500 (Shenzhen, China) automatic blood counting system. The blood samples for platelet parameters were collected in ethylenediaminetetraacetic acid tubes. Coagulation parameters including activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and D-dimer were measured with STAGO STA-R Evolution. The blood samples for coagulation parameters were collected in sodium citrate anticoagulation tubes.
Sta r evolution
Manufactured by Stago
Sourced in France
The STA-R Evolution is a coagulation analyzer designed for routine and specialized coagulation testing. It offers automated processing of coagulation tests and provides reliable and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Sta liquid anti xa, by Stago (2 mentions)
Bc 5500, by Mindray (1 mentions)
Cobas 8000, by Roche (1 mentions)
Coaguchek xs, by Roche (1 mentions)
Sta r, by Stago (1 mentions)
Hiscl tat, by Sysmex (1 mentions)
Hemosil liquid heparin, by Werfen (1 mentions)
Sta edoxaban calibrator, by Stago (1 mentions)
Sta edoxaban control, by Stago (1 mentions)
I stat 300, by Abbott (1 mentions)
Sta procoag ppl, by Stago (1 mentions)
Sodium citrate anticoagulant, by Sarstedt (1 mentions)
Xe 2100, by Sysmex (1 mentions)
20 protocols using sta r evolution
1
Platelet and Coagulation Measurement
2
Comprehensive COVID-19 Clinical Parameters
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The baseline clinical characteristics, including age, underlying disease, days from onset to admission, and clinical classifications were collected from electronic medical records, and all laboratory tests were performed according to the clinical needs of patients. The levels of electrolyte (Natrium: Na, Kalium: Ka, Chlorine: Cl, Calcium: Ca), highly sensitive cTnI (hs-cTnI), N-terminal pro b-type natriuretic peptide (NT-proBNP), blood urea nitrogen (BUN), creatinine (CRE), estimated glomerular filtration rate (eGFR), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and direct bilirubin (DBIL) measured by Roche COBAS8000 were recorded. Prothrombin time (PT) were measured by Stago STA-R EVOLUTION and the worst SpO2 within 24 h of admission were measured by ABL800. Moreover, the cytokine levels of 93 patients were tested according to the clinical needs of patients. All the blood parameters were assayed by clinical laboratory of Tongji Hospital.
3
Evaluation of CoaguChek XS INR Device
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CoaguChek XS (Roche Diagnostics) is a small, handheld device for the self-testing of INR. The device shows good analytic performance 19 with an analytical variation (CVa) of approximately 3% 20 and is easy for patients to use after thorough training. 20 Capillary blood from the finger is applied to a test strip placed in the POC device for analysis of the INR. The reagents SPA50/SPAþ (Stago, France) were used for analysis of INR in citrated plasma on the hospital instruments, STA-R and StaR Evolution (Stago). Standard procedures for internal and external controls were followed, and gave acceptable results. Results from the split-sample analysis (comparison of Coagu-Chek XS INR result with hospital INR result) showed that the INR values during the training period were within the acceptable limits given by the International Organization for Standardization for self-testing devices 21 (►Supplementary Fig. S1, available in the online version).
4
Coagulation Factors Assay via Citrated Plasma
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Blood was collected via carotid artery cannulas and mixed with 0.109 M sodium citrate. Plasma was prepared by centrifugation at 4,000 × g for 10 min to remove cellular elements. The activities of factors, APTT, and PT were detected using an automation coagulator STA-R Evolution (Stago, NJ, USA) following the standard protocol.
5
Biomarker Analysis in Acute Myocardial Infarction
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For all patients, the blood samples were analyzed by Clinical Laboratory Department of Beijing Friendship Hospital. The blood samples for platelet parameters were collected in ethylenediaminetetraacetic acid tubes and then measured with the SYSMEX XE-2100 (Kobe, Japan) automated blood counting system; the blood samples for coagulation parameters were collected in sodium citrate anticoagulation tubes and then measured with STAGO STA-R Evolution automated coagulation analyzer (Gennevilliers, France); blood concentrations of muscle-brain type isoenzyme of creatine kinase (CKMB), myoglobin (Myo), troponin I (TnI), and N-terminal pro-brain natriuretic peptide (NT-proBNP) were measured during admission and at 12-hour intervals during the first 5 days following the presentation of AMI (from symptom onset). Serum peak concentrations of the TnI, Myo, and CKMB levels were used for estimations of myocardial infarct size. 12 After 12-hour fasting, blood samples were taken from an antecubital vein to measure total cholesterol (TC), triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol (LDL-C), and fasting plasma glucose levels. For patients without AMI, the above markers were measured on the second day after admission.
6
Rivaroxaban Levels Determination
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Rivaroxaban levels were determined using an anti-factor Xa activity assay (STA®-Liquid Anti-Xa) using STA®-Rivaroxaban Calibrator and STA®-Rivaroxaban Controls on the STA-R Evolution® analyzer (Stago Diagnostica, Asnieres, France). External laboratory was used for a second measurement of anti-factor Xa activity assay.
7
Comprehensive Coagulation Biomarker Profiling
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Measurements of PT, APTT, FIB, TT, D‐dimer, FDP, and vWF were performed at Stago STA‐R Evolution. The reagents used were STA‐Neoplastin Cl Plus⑤, STA‐PTT Automate⑤, STA‐CaCl2 0.025 M, STA‐Fibrinogen⑤, STA‐Thrombin⑤, LIATEST FDP and STA‐von Willebrand factor⑤. The assays of APTT, PT, and TT were conducted using a clotting method. The quantitative determination of FIB concentration in plasma was made by the clotting method of Clauss. The quantitative determination of D‐dimer, FDP, and vWF concentration in plasma was determined by the immuno‐turbidimetric method.
Measurements of TAT, PIC, and TM were performed at Syxmex HSCL5000. The reagents used were HISCL TAT, HISCL PIC, and HISCL TM from Sysmex. The immuno‐turbidimetric method was used to quantitatively test the concentration of TAT, PIC, and TM.
Measurements of TAT, PIC, and TM were performed at Syxmex HSCL5000. The reagents used were HISCL TAT, HISCL PIC, and HISCL TM from Sysmex. The immuno‐turbidimetric method was used to quantitatively test the concentration of TAT, PIC, and TM.
8
Immunoturbidimetric D-Dimer Quantification
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Quantitation of DD levels was performed using the STA-R Evolution coagulation analyzer and was photometrically determined by the immunoturbidimetric method using the Liatest kit (Stago, Asnières, France). This test was performed according to the manufacturer's protocol.
9
Coagulation Assays for Thrombosis Risk
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The plasma-activated partial thromboplastin time (aPTT), prothrombin time (PT), thromboplastin time (TT), and fibrinogen levels from the blood samples were measured using Stago STA-R Evolution (Asnières-Sur-Seine, France). The factor X coagulation activity (FX: C) assay method involved a one-stage prothrombin time (PT) analysis using thromboplastin and factor X-deficient reagents from Diagnostic Stago (Asnières-Sur-Seine, France). A mixing study was performed to eliminate the presence of inhibitors. FX concentrations were determined using enzyme-linked immunosorbent assay (ELISA, human coagulation factor X ELISA kit; ELK Biotechnology, Wuhan, China). Quality controls for all tests were performed according to the manufacturer’s instructions.
10
Colorectal Cancer D-dimer Predictive Model
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The D-dimer level was measured by STA-R Evolution® (STAGO, Asnières, France) using the STA® - Liatest® D-Di kit (STAGO, Asnières, France) and analyzed to define the cut-off level for VTE high-risk patients with colorectal cancer. In the modeling cohort, clinical information was analyzed retrospectively when the patients were enrolled in the study, including age, gender, BMI, tumor stages, metastasis, pelvic or peritoneal effusion, underlying diseases, blood transfusion history (within three months prior to inclusion), surgery history (within three months prior to inclusion), therapeutic regimens and baseline laboratory data at diagnosis of colon cancer (carcinoembryonic antigen, hemoglobin, platelet count, white-cell count, D-dimer). The validation cohort was analyzed according to the RVTA scores, with several statistical indexes evaluated for prediction effectiveness. To further evaluate the calibration, the validation cohort was grouped based on the RVTA scores, and the number of expected VTE events in each group was compared to the number of observed VTE events. Subsequently, correlation analysis was conducted.
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