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Gentamycin

Manufactured by Sartorius
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Gentamycin is a laboratory product manufactured by Sartorius. It is a chemical compound used in cell culture and microbiology applications. Gentamycin functions as an antibiotic agent.

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Lab products found in correlation

Penicillin, by Sartorius (3 mentions) Glutamine, by Sartorius (3 mentions) Penicillin streptomycin, by Sartorius (2 mentions) Hydrocortisone, by Merck Group (2 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions) Insulin, by Merck Group (2 mentions) Trypsin edta, by Sartorius (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Cisplatin, by Merck Group (1 mentions) Rpmi 1640 medium, by Lonza (1 mentions) Kanamycin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Dmem f12, by Sartorius (1 mentions) D glucose, by Sartorius (1 mentions) Metronidazole, by Merck Group (1 mentions) Trypsin, by Sartorius (1 mentions) Streptomycin, by Sartorius (1 mentions) Trypan blue, by Sartorius (1 mentions) Glutamine, by Merck Group (1 mentions) Gentamycin, by Merck Group (1 mentions)

Close spelling variants of this product

Gentamycin Sulfate Solution Gentamycin sulfate

17 protocols using gentamycin

1

Ovarian Cancer Cell Culture and Drug Treatments

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SK-OV-3 human ovarian cancer (adenocarcinoma) cells from American Type Culture Collection (ATCC® HTB-77™) were cultured in RPMI-1640 medium (Lonza) supplemented with 10% (v/v) FBS (foetal bovine serum; Gibco®) and 50 µg/ml gentamycin (Biological Industries). TOV-21G human ovarian cancer (grade 3, stage III, primary malignant adenocarcinoma; clear cell carcinoma) cells from American Type Culture Collection (ATCC® CRL-11730™) were grown in the mixture (1:1) of MCDB-105 medium (Biological Industries) and M-199 Earle’s Salts Base medium (Biological Industries) supplemented with 15% (v/v) FBS (Gibco®) and 50 µg/ml gentamycin (Biological Industries). Both cell lines were cultivated at 37 °C in a humidified atmosphere of 95% air and 5% CO2.
Morin was obtained from Sigma-Aldrich, dissolved in DMSO (dimethyl sulfoxide; BioShop Canada Inc.) at a concentration of 50 mM and stored in – 20 °C. Cisplatin was acquired from Sigma-Aldrich, dissolved in 0.9% NaCl solution (Polpharma) at a concentration of 1 mg/ml (3333 mM), and stored in − 20 °C.
Bieg D., Sypniewski D., Nowak E, & Bednarek I. (2018). Morin decreases galectin-3 expression and sensitizes ovarian cancer cells to cisplatin. Archives of Gynecology and Obstetrics, 298(6), 1181-1194.
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2

Redifferentiation of Expanded Human Islet Cells

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Human islets were received 2–4 days following isolation from individual donors (Table 1). Islets were dissociated into single cells and cultured in CMRL 1066 medium containing 5.6 mM D-glucose and supplemented with 10% FCS (HyClone), 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml gentamycin, and 5 μg/ml amphotericine B (Biological Industries) (growth medium) as described [1 (link)]. The cultures were refed twice a week and split 1:2 once a week. For redifferentiation, expanded cells in passages 5–7 were trypsinized and seeded in ultra-low attachment plates with Redifferentiation Cocktail (RC) for 4–8 days as previously described [5 (link)]. The medium was replaced every two days.
Nathan G., Kredo-Russo S., Geiger T., Lenz A., Kaspi H., Hornstein E, & Efrat S. (2015). MiR-375 Promotes Redifferentiation of Adult Human β Cells Expanded In Vitro. PLoS ONE, 10(4), e0122108.
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3

Ceftriaxone Susceptibility in Borrelia Mutants

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The study was conducted using previously characterized B. burgdorferi strains [16 (link)]. dbpAB knock out strain, ΔdbpAB/E22/1 (ΔdbpAB), the DbpA and B expressing strain, ΔdbpAB/dbpAB/2 (ΔdbpAB/dbpAB), the DbpA expressing strain, ΔdbpAB/dbpA/1 (ΔdbpAB/dbpA), and the DbpB expressing strain, ΔdbpAB/dbpB/1 (ΔdbpAB/dbpB) in B. burgdorferi B31 5A13 background are identical in all other aspects of their genetic composition but differ in the ability to express DbpA and/or B. The spirochetes were cultivated in Barbour-Stoenner-Kelly II (BSK II) medium containing kanamycin (200 μg/ml, Sigma-Aldrich, St. Louis, MO, USA) and gentamycin (50 μg/ml, Biological Industries, Beit-Haemek, Israel) at 33°C. The minimal inhibitory concentration (MIC) of ceftriaxone was determined by culturing ΔdbpAB/dbpAB and ΔdbpAB in two-fold dilutions of the antibiotic in BSK II medium covering a concentration range of 0.5–0.002 μg/ml. Dark-field microscopy was used to detect the growth of the bacteria.
Salo J., Jaatinen A., Söderström M., Viljanen M.K, & Hytönen J. (2015). Decorin Binding Proteins of Borrelia burgdorferi Promote Arthritis Development and Joint Specific Post-Treatment DNA Persistence in Mice. PLoS ONE, 10(3), e0121512.
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4

Osteoblast Cell Culture on Ti Alloys

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MG 63 (human osteoblast cell line) was obtained from NCCS Pune, India, and was kept in Dulbecco's Modified Eagle's medium (DMEM, GIBCO, Invitrogen Corp). The medium contained high glucose with pyridoxine HCl, sodium pyruvate, L-glutamine sodium bicarbonate, and was supplemented with 100% fetal bovine serum (FBS, Biological Industries, Haemek, Israel), 100 IU/ml penicillin (Himedia), and gentamycin 20 μg/ml (Nicholas). The cells were seeded into tissue culture flasks and were allowed to grow in a controlled humidified incubator with 5% CO2 and 98% humidity at 37°C. All the samples of Ti alloy were sterilized by soaking in Extran MAO3 phosphate-free detergent solution (Merck Industries). Subsequently, they were autoclaved at a pressure of 15 lbs for 30 min. Then apart from Group 1, samples of Groups 3, 4, and 5 were irradiated to UV treatment for 12 h, 24 h, and 48 h, respectively.
0.5 × 106 osteoblast cells were seeded on each test sample kept in a 12-well plate. Each experiment was performed four times in triplicate, and standard deviation and variance were calculated. The growth of cells was examined at different time intervals of 12, 24, and 48 h in a CO2 incubator (Cytoperm® Heraeus®) at 37°C in DMEM medium containing 10% FBS and 1% antibiotics.
Yadav A., Yadav R., Gupta A., Baranwal A., Bhatnagar A, & Singh V. (2017). Effect of Ultraviolet Irradiation on the Osseointegration of a Titanium Alloy with Bone. Contemporary Clinical Dentistry, 8(4), 571-578.
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5

Optimized Cell Culture Medium Preparation

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The culture medium was prepared from 90% Dulbecco's modified eagle medium-nutrient mixture F-12 (DMEM-F12), 10% heat-denatured fetal calf serum (FCS), 6 g/L D-glucose, 2 nM glutamine, 25 μg/mL gentamycin, and 50 ng/mL IGF-I (all purchased from Biological Industries, Israel).
Ziv-Polat O., Shahar A., Levy I., Skaat H., Neuman S., Fregnan F., Geuna S., Grothe C., Haastert-Talini K, & Margel S. (2014). The Role of Neurotrophic Factors Conjugated to Iron Oxide Nanoparticles in Peripheral Nerve Regeneration: In Vitro Studies. BioMed Research International, 2014, 267808.
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6

Bacterial Invasion Assay in Differentiated THP1 Cells

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1x106 shCtrl and shVCL THP1 cells/well were differentiated with PMA for 72 h followed by challenge with P. gingivalis (MOI 10) for 1 h. Cells were treated with LY294002, LY303511 or anti-TLR2 (clone T2.5) vs. IgG1 isotype control (Biolegend) for 1 h prior to challenge with P. gingivalis. Extracellular bacteria were killed by treatment with 6 mg/mL Metronidazole (Sigma-Aldrich) and 0.3 mg/mL Gentamycin (Biological Industries) for 1 h. The medium was then replaced with complete DMEM, cells were incubated for 1 h and then lysed in sterile distilled water for 20 min. Lysates were plated on blood agar (Novamed, Jerusalem, Israel) in anaerobic conditions for 7 to 10 days and CFU were enumerated.
Pandi K., Angabo S., Gnanasekaran J., Makkawi H., Eli-Berchoer L., Glaser F, & Nussbaum G. (2023). Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion. PLOS Pathogens, 19(4), e1011284.
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7

Cytotoxicity and Mitochondrial Assay Reagents

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Materials for cell culture including media, serum, gentamycin, glutamine, penicillin, streptomycin, saline, Trypan blue, and trypsin were purchased from Biological Industries.

Cytotoxicity detection kit (LDH) (Roche Pharmacuticals, Bazel, Switzerland).

The fluorescent dye JC-1 was obtained from Calbiochem (Merck, Darmstadt, Germany).

The NAO was obtained from Sigma.

Cell culture vessels (Corning Life Sciences, Ramat-Gan, Israel).

Vainshtein A., Veenman L., Shterenberg A., Singh S., Masarwa A., Dutta B., Island B., Tsoglin E., Levin E., Leschiner S., Maniv I., Pe’er L., Otradnov I., Zubedat S., Aga-Mizrachi S., Weizman A., Avital A., Marek I, & Gavish M. (2015). Quinazoline-based tricyclic compounds that regulate programmed cell death, induce neuronal differentiation, and are curative in animal models for excitotoxicity and hereditary brain disease. Cell Death Discovery, 1, 15027-.
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8

Isolation of Normal Breast Cell Types

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Normal breast biopsies were collected with consent from women undergoing reduction mammoplasty for cosmetic reasons. The use of human material has been reviewed by the Regional Scientific Ethical Committees (Region Hovedstaden) and approved with reference to H-2–2011–052. Normal breast tissue was prepared as described25 (link). In brief, normal breast specimens were minced with opposing scalpels and treated overnight at 37 °C with rotation in collagenase (Type 3, Worthington Biochemical Corporation, 900 U ml−1 in DMEM/F-12 (Life Technologies, catalogue no. 21041) with 2 mM glutamine (Sigma, G7513) and 50 μg ml−1 gentamycin (Biological Industries)) before differential centrifugation to aspirate dissolved fat and to isolate epithelial organoids and fibroblasts. These were then either used directly or frozen in liquid nitrogen for later use.
Fridriksdottir A.J., Kim J., Villadsen R., Klitgaard M.C., Hopkinson B.M., Petersen O.W, & Rønnov-Jessen L. (2015). Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nature Communications, 6, 8786.
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9

Culturing Brain Endothelial Cells

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‘Plating medium’ was composed as follows: 10% newborn calf serum, 2 mM L-glutamine, penicillin/streptomycin (100 units/mL and 0.1 mg/mL respectively), 0.1 mg/mL gentamycin (Biological industries, Kibbutz Beit-Haemek, Israel) in Earl’s Medium 199 (Sigma). ‘Assay medium’ contained: 2 mM L-glutamine, penicillin/streptomycin (100 units/mL and 0.1 mg/mL respectively), 0.1 mg/mL gentamycin, 550 nM hydrocortisone (Sigma) in Dulbecco-modified Earl’s medium (DMEM) diluted 1:1 in Ham’s F12 medium (Biological industries, Israel). For growing hCMEC/D3 (brain endothelial cell line) EGM-1 medium was used: EGM-2 medium (Lonza) supplemented with FBS, bFGF, gentamycin, ascorbic acid and hydrocortisone according to the manufacture instructions (EGM-2 SingleQuots, LONZA).
Cooper I., Cohen-Kashi Malina K., Levin Y., Gabashvili A., Mohar B., Cagnotto A., Salmona M, & Teichberg V.I. (2022). Cytoskeleton Elements Contribute to Prion Peptide-Induced Endothelial Barrier Breakdown in a Blood–Brain Barrier In Vitro System. International Journal of Molecular Sciences, 23(20), 12126.
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10

Inducing Brain-like Phenotype in ECs

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BLECs and pericytes were grown in endothelial cell medium (ECM) (Sciencell) supplemented with 5% fetal calf serum (Gibco), ECGS supplements, and 50 μg/ml gentamycin (Biological industries). In order to induce brain-like phenotype, ECs monolayers were grown in medium containing 2/3 ECM and 1/3 pericytes-conditioned medium (ECM medium collected after 3 days culture with pericytes), as previously published [26 (link), 27 (link)].
Israelov H., Ravid O., Atrakchi D., Rand D., Elhaik S., Bresler Y., Twitto-Greenberg R., Omesi L., Liraz-Zaltsman S., Gosselet F., Schnaider Beeri M, & Cooper I. (2020). Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair. Journal of Neuroinflammation, 17, 267.
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