Traxoprodil

Traxoprodil (5, 10, 20, and 40 mg/kg, Sigma-Aldrich) was suspended in a 1 % aqueous solution of Tween 80 (POCH), whereas imipramine hydrochloride (15 and 30 mg/kg, Sigma-Aldrich), fluoxetine hydrochloride (5 mg/kg, Sigma-Aldrich), escitalopram oxalate (2 mg/kg, Sigma-Aldrich), reboxetine mesylate (2.5 mg/kg, Abcam Biochemicals), WAY 100,635 (0.1 mg/kg, Sigma-Aldrich), and ritanserin (4 mg/kg, Sigma-Aldrich) were dissolved in physiological saline (0.9 % NaCl). The solutions/suspension were prepared immediately prior to the experiments and were administered intraperitoneally (i.p.) 60 min before testing. The doses and pretreatment schedules were selected on the basis of the literature data and the results of our previous experiments (Poleszak et al. 2005 (link), 2007a (link), 2011 (link), 2013 (link); Szewczyk et al. 2002 (link), 2009 (link)). Animals from the control groups received i.p. injections of the vehicle (saline). The volume of all administered solutions/suspension was 10 ml/kg.

Traxoprodil (10 mg/kg, Sigma-Aldrich) was suspended in a 1% aqueous solution of Tween 80 (POCH), whereas desipramine hydrochloride (10 mg/kg, Sigma-Aldrich), paroxetine hydrochloride (0.5 mg/kg, Sigma-Aldrich), milnacipran hydrochloride (1.25 mg/kg, Abcam Biochemicals), and bupropion hydrochloride (10 mg/kg, Abcam Biochemicals), were dissolved in physiological saline (0.9% NaCl). The solutions/suspension were prepared immediately prior to the experiments and were administered intraperitoneally (i.p.) 60 min before testing. The doses and pretreatment schedules were selected on the basis of the literature data and the results of our previous experiments (Peng et al. 2007 (link); Piotrowska et al. 2013 (link); Poleszak et al. 2015 (link); Szopa et al. 2016 (link)). Animals from the control groups received i.p. injections of the vehicle (saline). The volume of all administered solutions/suspension was 10 ml/kg.

l-Glutamine, cAMP, apotransferrin, glucose, insulin, putrescine, selenium, progesterone, ascorbic acid, AP5, domoic acid, ketamine, quinolinic acid, traxoprodil, nicotine, dextromethorphan (DXM), MK801, and bicuculline were purchased from Sigma (St. Louis, USA). DMEM/F12, knockout serum replacement, Neurobasal, N2 supplement, B27 without ascorbic acid supplement, Glutamax, NEAA, ß-mercaptoethanol, HBSS, Triton-X-100, PBS, H-33342, FBS, Trizol, Fluo-4 Direct™ Calcium Assay Kit were purchased from ThermoFisher Scientific (Waltham, USA). GDNF, noggin, BDNF and NGF were purchased from R&D systems (Minneapolis, USA). Dorsomorphin, SB-431642, SU5402, ibotenic acid, NMDA, S-AMPA, phencyclidine (PCP), and kainate were purchased from Tocris (Bristol, UK). Chir99021 was purchased from Axon Medchem (Groningen, Netherlands); DAPT was purchased from Merck Millipore (Billerica, USA). Matrigel was purchased from Corning (Corning, USA); iScript and SsoFast™ EvaGreen® Supermix were purchased from BioRad (Hercules, USA). EdU click Kit was purchased from baseclick (Neuried, Germany). Purmorphamine (PMA) was purchased from Enzo (Farmingdale, USA). Veratridine was purchased from Alomone labs (Jerusalem, Israel). TGFß was purchased from Peprotech (Rocky Hill, USA).