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Mouse igg2a

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Sourced in China, United States

Mouse IgG2a is an immunoglobulin G (IgG) antibody subclass produced by mice. It is a common tool used in various immunological and biochemical applications.

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Lab products found in correlation

Mouse igg1, by Agilent Technologies (2 mentions) Mouse anti cd68 clone kp1, by Agilent Technologies (2 mentions) Goat polyclonal anti nkp46, by R&D Systems (2 mentions) Mouse anti cd56 clone 123c3, by Agilent Technologies (2 mentions) Mouse anti neutrophil defensins clone d21, by Leica (2 mentions) Mouse anti cd16 clone 2h7, by Leica (2 mentions) Rabbit anti cd32 clone epr6657 2, by Abcam (2 mentions) Mouse anti cd20 clone l26, by Agilent Technologies (2 mentions) Rabbit polyclonal anti cd3, by Agilent Technologies (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions) Interleukin 2 (il 2), by BioLegend (1 mentions) Donkey serum, by Merck Group (1 mentions) α synuclein, by Abcam (1 mentions) Foxa2, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse igg1, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg2b alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Tyrosine hydroxylase, by Merck Group (1 mentions) Alexa fluor 488 goat anti mouse igg2a, by Thermo Fisher Scientific (1 mentions)

7 protocols using mouse igg2a

1

Immunohistochemical Profiling of Immune Cells

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Antigen-retrieval and signal detection were performed as previously described 20 (link). The following primary Abs were used: goat polyclonal anti-NKp46 (R&D Systems), mouse anti-CD56 (clone 123C3, Dako), mouse anti-CD68 (clone KP1, Dako), mouse anti-neutrophil defensins (clone D21, Leica Biosystems), mouse anti-CD16 (clone 2H7, Leica Biosystems), rabbit anti-CD32 (clone EPR6657(2), Abcam), mouse anti-CD20 (clone L26, Dako), rabbit polyclonal anti-CD3 (Dako). The following control Abs were used: mouse IgG1 (Dako), goat IgG (R&D Systems), mouse IgG2a (Abcam) and rabbit IgG (Abcam).
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
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2

Immunohistochemical Profiling of Immune Cells

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Antigen-retrieval and signal detection were performed as previously described 20 (link). The following primary Abs were used: goat polyclonal anti-NKp46 (R&D Systems), mouse anti-CD56 (clone 123C3, Dako), mouse anti-CD68 (clone KP1, Dako), mouse anti-neutrophil defensins (clone D21, Leica Biosystems), mouse anti-CD16 (clone 2H7, Leica Biosystems), rabbit anti-CD32 (clone EPR6657(2), Abcam), mouse anti-CD20 (clone L26, Dako), rabbit polyclonal anti-CD3 (Dako). The following control Abs were used: mouse IgG1 (Dako), goat IgG (R&D Systems), mouse IgG2a (Abcam) and rabbit IgG (Abcam).
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
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3

Immunohistochemical Evaluation of MAGE-A4 in Human Tumors

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Human tumor biopsies (n=481) of different histologic subtype were obtained as formalin-fixed paraffin-embedded blocks. MAGE-A4-negative A549 cells and MAGE-A4-transduced A549 cells were used as controls, and their MAGE-A4 expression status was confirmed via WESTM, qPCR, and/or Nanostring experiments (data not shown). All blocks including the cell pellets and tissues were processed to slides (5 µm thick sections) and stained with a mouse anti-human MAGE-A4 monoclonal antibody (ThermoFisher, OTI1F9, cat# MA5-26118, 0.04 µg/mL) or an irrelevant isotype control (mouse IgG2a, Abcam ab#18413) according to standard protocols using a Biocare Intellipath autostaining system. An exception was triple-negative breast cancer, which was done manually. Slides were developed with DAB and counterstained with hematoxylin.
Slides were evaluated by the study pathologist using a standard bright-field microscope. The intensity of tumor cell immunoreactivity was scored 0=no, 1=low/minimal, 2=moderate, and 3=marked membranous and/or cytoplasmic immunoreactivity. The percentages of cells assigned to each score were estimated and an H-score assigned: H-score=[1*(%cells score1)+2*(%cells score2)+3*(%cells score3)]. Images were captured using the Panoramic 3D Histech scanner and CaseViewer digital pathology software (3DHISTECH).
Davari K., Holland T., Prassmayer L., Longinotti G., Ganley K.P., Pechilis L.J., Diaconu I., Nambiar P.R., Magee M.S., Schendel D.J., Sommermeyer D, & Ellinger C. (2021). Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy. Journal for Immunotherapy of Cancer, 9(3), e002035.
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4

Quantifying Immune Biomarkers in Cell Culture

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Concentrations of IFN-γ, IgG2a and IL-2 in the cell culture supernatants were determined using commercially available ELISA kits specific for bovine IFN-γ (Bethyl, Montgomery, TX), bovine IL-2 (Hiton, Tianjin, China) and mouse IgG2a (Abcam, Cambridge, UK). The ELISAs were performed according to the manufacturer’s instructions.
Song Q.J., Weng X.G., Cai D.J., Zhang W, & Wang J.F. (2016). Forsythoside A Inhibits BVDV Replication via TRAF2-Dependent CD28–4-1BB Signaling in Bovine PBMCs. PLoS ONE, 11(9), e0162791.
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5

Phenotyping Antigen-Specific T Cells

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Splenocytes from VV gp120MN-immunized mice were distributed to a 96 well plate (106 cells/200 μL) and incubated for 15 min with one of the following anti-mouse mAbs: H2-Dd (34-2-12), H2-Ld/H2-Db (28-14-8), H2-Kd (SF1-1.1) (Biolegend), or an isotype control, mouse IgG2a (AbCam). After incubation, the indicated peptide was added at 1.5 μg/ml, the cells were cultured with peptide for 6 hours and then were stained for CD3, CD4, CD8, IL2, IFNγ (Biolegend), AquaBlue or Yellow Viability Dye (Life Technologies) and TNF (BD Pharmigen). Intracellular cytokine production was quantified using the previously described method (27 (link)–29 (link)). Stained cells were analyzed using a BD LSR II flow cytometer with FlowJo software.
Frey B.F., Jiang J., Sui Y., Boyd L.F., Yu B., Tatsuno G., Billeskov R., Solaymani-Mohammadi S., Berman P.W., Margulies D.H, & Berzofsky J.A. (2018). Effects of cross-presentation, antigen processing, and peptide binding in HIV evasion of T cell immunity. Journal of immunology (Baltimore, Md. : 1950), 200(5), 1853-1864.
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6

Immunofluorescent Labeling of Dopaminergic and Alpha-Synuclein Proteins

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Cells were fixed with 4% PFA and blocked with 2% donkey serum (Sigma) in PBS‐T (0.1% Triton X‐100 (Fisher) in PBS (Thermo Fisher Scientific)). Primary antibodies used include tyrosine hydroxylase (1:1,000, rabbit, Millipore), FOXA2 (1:100, goat, Santa Cruz), α‐synuclein (1:250, mouse IgG2a, Abcam), α‐synuclein (1:250, mouse IgG1, BD), phosphoserine‐129 α‐synuclein (pS129‐αSyn, 1:1,000, rabbit, Abcam) and β‐III tubulin (1:1,000, mouse IgG2b, Abcam). Secondary antibodies including donkey anti‐rabbit IgG Alexa Fluor‐488 (Thermo Fisher Scientific), donkey anti‐goat IgG Alexa Fluor‐568 (Thermo Fisher Scientific), donkey anti‐mouse IgG Alexa Fluor‐647 (Abcam), goat anti‐mouse IgG2a Alexa Fluor‐488 (Thermo Fisher Scientific), goat anti‐mouse IgG1 Alexa Fluor‐488 (Thermo Fisher Scientific), goat anti‐mouse IgG2b Alexa Fluor‐647 (Thermo Fisher Scientific) were used at 1:1,000, while DAPI (Thermo Fisher Scientific) was used at 1:10,000. Images were acquired on the Axio Observer (Zeiss) or the Eclipse Ti‐E (Nikon) microscope.
Chen Y., Dolt K.S., Kriek M., Baker T., Downey P., Drummond N.J., Canham M.A., Natalwala A., Rosser S, & Kunath T. (2018). Engineering synucleinopathy‐resistant human dopaminergic neurons by CRISPR‐mediated deletion of the SNCA gene. The European Journal of Neuroscience, 49(4), 510-524.
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7

Multiparametric Flow Cytometry for Glycosaminoglycans

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The following antibodies were used (all anti-human): Heparan Sulfate (clone F58-10E4) (Amsbio), digested Heparan (clone F69-3G10) (Amsbio), Syndecan 1 (DL-101) (Santa Cruz), Syndecan 2-FITC (H-7) (Santa Cruz), Syndecan 3 (M-300) (Santa Cruz), Syndecan 4 (clone F94-8G3), CD207-PE (langerin) mouse IgG1 (#IM3577) (BeckmanCoulter, USA), CD1a-APC mouse IgG1 (BD Biosciences, San Jose, CA, USA) CD1a-PE (clone SK9) mouse IgG2b (BD Bioscience), HLA-B27-FITC (clone HLA-ABC-m3), mouse IgG2a (Abcam), HLA-DR-FITC (clone G46-6), mouse IgG2b (BD Bioscience), CD80-PE, mouse IgG1 (BD Pharmingen), CD83-PE, mouse IgG1 (eBioscience), CD86-FITC, mouse IgG1 (BD Pharmingen), FITC-conjugated goat-anti-mouse IgM (#31992) (Invitrogen), AF488-conjugated goat-anti-mouse IgG1 (#A21121) (Invitrogen), AF488-conjugated donkey-anti-mouse IgG2b (Invitrogen).
The following reagents were used: Unfractionated (UF) heparin, 5.000 I.E./ml (LEO). Low Molecular Weight (LMW) heparins: dalteparin, 10.000 IE anti-Xa/ml (Pfizer), tinzaparin, 10.000 IE anti-X1/0.5ml (LEO), enoxaparin, 100 mg/ml (Sanofi). 4-Nitrophenyl β-D-xylopyranoside (PNP-Xyl, 2001-96-9) (SigmaAldrich). Heparinase III from Flavobacterium heparium, EC 4.2.2.8, Batch 010 (Amsbio). 123Count eBeads, REF# 01-1234-42, LOT# E133305, 1.011.000 eBeads/ml (eBioscience).
Nijmeijer B.M., Eder J., Langedijk C.J., Kaptein T.M., Meeussen S., Zimmermann P., Ribeiro C.M, & Geijtenbeek T.B. (2020). Syndecan 4 Upregulation on Activated Langerhans Cells Counteracts Langerin Restriction to Facilitate Hepatitis C Virus Transmission. Frontiers in Immunology, 11, 503.
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