Fasting glucose, total, and high-density lipoprotein (HDL) cholesterol levels were measured in plasma using Infinity™ Glucose Hexokinase Liquid Stable Reagent, Infinity™ Cholesterol, and HDL-CHOLESTEROL Plus Direct method, respectively (Thermo Fisher Scientific Inc., Waltham, MA, USA). Insulin levels were quantified by enzyme immunoassay kits (Mercodia AB, Uppsala, Sweden). Lipopolysaccharide binding protein (LBP) was measured by mouse LBP ELSA kit (Hycult Biotech Inc., Wayne, NJ, USA). Serum amyloid A (SAA) levels were determined by “PHASE” ™ Murine Serum Amyloid A Assay (Tridelta Development Ltd., Maynooth, Ireland). Cytokine levels were measured in plasma (dilution 1:1) using Meso Scale Discovery V-PLEX proinflammatory panel one kit (#K15048D-1) as described in section 2.8. In this case, the calculated detection limits were the following: IFN-γ (0.00988–909 pg/mL), IL-1β (0.374–1,765 pg/mL), IL-2 (0.194–2,595 pg/mL), IL-4 (0.0266–1,680 pg/mL), IL-5 (0.224–1,027 pg/mL), IL-6 (0.335–5,440 pg/mL), IL-10 (0.103–3,930 pg/mL), IL-12p70 (1.79–31,400 pg/mL), KC/GRO (0.0968–1,960 pg/mL), TNF-α (0.0256–619 pg/mL).
Hdl cholesterol plus direct
Manufactured by Thermo Fisher Scientific
Sourced in United States
HDL-CHOLESTEROL Plus Direct is a laboratory assay used for the quantitative determination of high-density lipoprotein (HDL) cholesterol in human serum or plasma samples. The assay provides a direct measurement of HDL cholesterol levels without the need for sample pretreatment or precipitation steps.
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2 protocols using hdl cholesterol plus direct
1
Metabolic and Inflammatory Biomarkers
2
Metabolic and Inflammatory Biomarkers
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Fasting glucose, total, and high-density lipoprotein (HDL) cholesterol levels were measured in plasma using Infinity™ Glucose Hexokinase Liquid Stable Reagent, Infinity™ Cholesterol, and HDL-CHOLESTEROL Plus Direct method, respectively (Thermo Fisher Scientific Inc., Waltham, MA, USA). Insulin levels were quantified by enzyme immunoassay kits (Mercodia AB, Uppsala, Sweden). Lipopolysaccharide binding protein (LBP) was measured by mouse LBP ELSA kit (Hycult Biotech Inc., Wayne, NJ, USA). Serum amyloid A (SAA) levels were determined by “PHASE” ™ Murine Serum Amyloid A Assay (Tridelta Development Ltd., Maynooth, Ireland). Cytokine levels were measured in plasma (dilution 1:1) using Meso Scale Discovery V-PLEX proinflammatory panel one kit (#K15048D-1) as described in section 2.8. In this case, the calculated detection limits were the following: IFN-γ (0.00988–909 pg/mL), IL-1β (0.374–1,765 pg/mL), IL-2 (0.194–2,595 pg/mL), IL-4 (0.0266–1,680 pg/mL), IL-5 (0.224–1,027 pg/mL), IL-6 (0.335–5,440 pg/mL), IL-10 (0.103–3,930 pg/mL), IL-12p70 (1.79–31,400 pg/mL), KC/GRO (0.0968–1,960 pg/mL), TNF-α (0.0256–619 pg/mL).
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