C myc 9e10

SOX2 (D6D9), OCT4 (C30A3), γH2AX (Ser139), CDK1, CDK2, p15INK4B, Cyclin D1 (DSS6), p-Aurora A (T288), p-Aurora B (T232, 1:1000), Aurora B, c-Myc (9E10) and Phospho-Chk2 (Thr68), Phospho-ATM (Ser198), Phospho p53 (Ser 15) antibodies were purchased from Cell Signalling Technology. GFP (clone B-2) antibody was purchased from Santa Cruz Biotechnology. Primary antibodies are used in 1:200 dilution for Immunofluorescence assay and 1:1000 dilution for Western blotting. GAPDH (clone MAB374, 1:4000) antibody was purchased from Millipore.
Horse radish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit secondary antibodies (1:5000) were purchased from Jackson ImmunoResearch. Alexa Fluor 488 goat anti-mouse antibody and Alexa Fluor 568 goat anti-rabbit antibody (1:200) were purchased from Life Technologies. Anti-PRL3 monoclonal antibody (mAb) (clone 318, 1:200 for Immunofluorescence and 1:2000 for western blot) was generated in-house. PRL3-zumab was engineered based on the original framework of murine anti-PRL3 mAb (clone 318).

Cells were extracted by direct lysis into SDS‐urea electrophoresis sample buffer, and western blotting was carried out as previously described [52 (link)]. To expose epitopes, membranes to be probed for ubiquitin were boiled in de‐ionised water for 30 min before blocking. The primary antibodies used were p53 (SAPU), Scottish Antibody Production Unit (Carluke, UK); MDM2 (4B2), Moravian‐Biotechnology (Brno, Czech Republic); S5A (14899‐1‐AP), Proteintech Group (Rosemont, IL, USA); ADRM1 (ab56852), RAD23A (EPR4817, ab108591), RAD23B (ab3835), HA tag (HA.C5, ab18181), NOXA (114C307, ab13654) and beta‐actin (ab8226), Abcam (Cambridge, UK); Ubiquitin (P4D1‐A11, 05‐944), p21 (EA10, OP64/Ab‐1) and GFP (mixture of clones 7.1 and 13.1, 11814460001), Merck Group; c‐MYC (9E10), prepared in house and MCL‐1 (D35A5, #5453), Cell Signaling Technology (Danvers, MA, USA). Suitable exposures of western blots were quantified by densitometry using Quantity One, Bio‐Rad (Hercules, CA, USA). SeeBlue Plus2 protein standards were obtained from Thermo Fisher Scientific.