Cells proliferation was analyzed using CyQUANT® dye-binding solution (Invitrogen) according to the manufacturer’s protocol. This assay is a highly sensitive fluorescence-based assay that determines the number of healthy, stable cultured cells [38 (link)]. The fluorescence intensity of each sample was measured at the excitation and emission wavelengths of 485 and 535 nm, respectively.
Cyquant dye binding solution
Manufactured by Thermo Fisher Scientific
The CyQUANT® dye-binding solution is a reagent used for quantifying cellular DNA content. It binds to DNA and emits a fluorescent signal that can be measured using a fluorometer or plate reader. The intensity of the fluorescent signal is proportional to the amount of DNA present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cyquant dye binding solution
1
Quantifying Cell Proliferation via CyQUANT Assay
2
Cell Proliferation Quantification
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