Log In Sign up
The largest database of trusted experimental protocols

Anti mouse cd11c pecy7 clone n418

Manufactured by BioLegend
Visit Supplier

Anti-mouse CD11c-PECy7 (clone N418) is a fluorochrome-conjugated antibody used to detect and analyze mouse CD11c-positive cells by flow cytometry. CD11c is a marker for dendritic cells in mice.

Automatically generated - may contain errors

Lab products found in correlation

Efluor 506, by Thermo Fisher Scientific (1 mentions) Anti mouse cd16 cd32, by BioLegend (1 mentions) Fortessa flow cytometer, by FlowJo (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Anti mouse cd16 cd32 clone 93, by BioLegend (1 mentions)

Spelling variants of this product

Anti-CD11c (97 citations) CD11c (N418, (54 citations) CD11c (clone N418, (33 citations) Anti-CD11c (N418, (25 citations) Anti-CD11c (clone N418, (24 citations) Anti-mouse CD11c (15 citations) Clone N418 (11 citations) Anti-mouse CD11c (clone N418; (5 citations) Anti-mouse CD11c (N418, (4 citations)

2 protocols using anti mouse cd11c pecy7 clone n418

1

Curdlan-AL Induces BMDC Maturation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Day 6 BMDC were stimulated for 24 hours in the presence or absence of curdlan-AL (100 μg/ml). BMDC were harvested stained for anti-mouse CD11c-PECy7 (clone N418; Biolegend), MHCcII I-Ab-FITC (clone AF6-120.1; Biolegend), CD40-APC (clone 3/23; Biolegend), and CD86-Brilliant Violet 650 (clone GL-1; Biolegend) and fixable viability dye eFluor-506 (eBioscience) in PBS containing anti-mouse CD16/CD32 (Biolegend). Expression of maturation markers was determined by gating on live, singlet, CD11c+ cells and gates were set using fluorescence minus one controls. Cells were fixed in FACS buffer (PBS, 5% FBS, 0.1% NaN3) containing 1% PFA and acquired using a Becton Dickinson Fortessa flow cytometer and data analysed using FlowJo Version 8.7.
Purvis H.A., Clarke F., Jordan C.K., Blanco C.S., Cornish G.H., Dai X., Rawlings D.J., Zamoyska R, & Cope A.P. (2017). Protein tyrosine phosphatase PTPN22 regulates IL‐1β dependent Th17 responses by modulating dectin‐1 signaling in mice. European Journal of Immunology, 48(2), 306-315.
+ Open protocol
+ Expand
2

Characterization of Mouse Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDCs were harvested and stained using anti-mouse CD11c-PE/Cy7 (clone N418; Biolegend), anti-mouse CD11b-APC/Cy7 (clone M1/70; Biolegend), MHC class II I-Ab-FITC (clone AF6-120.1; Biolegend), CD206-PE (clone C068C2; Biolegend) and Zombie UV Fixable Viability (Biolegend) in PBS containing anti-mouse CD16/CD32 (clone 93; Biolegend). Cells were washed with FACS buffer (5% FBS, 1 mM EDTA, 0.01% NaN3 in PBS) and fixed using 1% paraformaldehyde (PFA, Electron Microscopy Services) in PBS.
Clarke F., Jordan C.K., Gutiérrez-Martinez E., Bibby J.A., Sanchez-Blanco C., Cornish G.H., Dai X., Rawlings D.J., Zamoyska R., Guermonprez P., Cope A.P, & Purvis H.A. (2017). Protein tyrosine phosphatase PTPN22 is dispensable for dendritic cell antigen processing and promotion of T-cell activation by dendritic cells. PLoS ONE, 12(10), e0186625.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!