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Vimentin

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Vimentin is a protein that is commonly used as a marker for mesenchymal cells in immunohistochemistry and flow cytometry applications. It is a type III intermediate filament protein that is involved in maintaining the structural integrity of cells.

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Lab products found in correlation

Iridium dna intercalator, by Standard BioTools (1 mentions) Nbc1 18380, by Novus Biologicals (1 mentions) Heparin sulfate, by STEMCELL (1 mentions) Fibroblast growth factor 2 (fgf2), by Cell Signaling Technology (1 mentions) Phospho frs2, by Cell Signaling Technology (1 mentions) Pfrs2 y196, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Tgf β, by Cell Signaling Technology (1 mentions) Anti fgfr1, by Cell Signaling Technology (1 mentions) Phospho fgfr, by Cell Signaling Technology (1 mentions) Anti frs2, by Abcam (1 mentions) Phospho plcγ, by Cell Signaling Technology (1 mentions) Pfgfr1, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp linked rabbit igg secondary antibody, by Cell Signaling Technology (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) Hif 1α, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions)

2 protocols using vimentin

1

Multiparametric Tissue Profiling with IMC

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Iridium DNA Intercalator (Fluidigm, 201192A) and metal-labeled antibodies pan-keratin (Nd 148, Fluidigm 3148020D), cytokeratin 7 (Dy 164, Fluidigm 3164028D), Ki-67 (Er 168, Fluidigm 3168022D), HLA-DR (Yb 174, Fluidigm 3174023D), α-SMA (Pr 141, Fluidigm 3141017D), vimentin (Nd 143, Fluidigm 3143029D), and collagen I (Tm 169, Fluidigm 3169023D) were purchased from Fluidigm. Tissue microarray (TMA) samples were purchased from BioMax (PA807). Recombinant mortalin was purchased from Novus Biologicals (NBC1-18380).
Yoon S., Li H., Quintanar L., Armstrong B, & Rossi J.J. (2020). Uncovering Differently Expressed Markers and Heterogeneity on Human Pancreatic Cancer. Translational Oncology, 13(3), 100749.
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2

FGF2 and FGFR1 Signaling Pathway Assay

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Recombinant FGF2 was purchased from Gibco (#PHG0024). Heparin sulfate was purchased from StemCell Technologies (#07980). The following antibodies were used (all primary antibodies are rabbit derived unless otherwise noted): anti-FGFR1 (#9740), phospho-FGFR (Y653/654, #3476, mouse derived), phospho-FRS2 (Y436, #3861; Y196, #3864), PLCγ (#5690), phospho-PLCγ (Y783, #2821), ERK (#4695), phospho-ERK (T202/Y204 #4370), Akt (#4691), phospho-Akt (S473 #4060), STAT3 (#4904), phospho-STAT3 (Y705, #9145), E-cadherin (#3195), Vimentin (#5741), ZEB1 (#3396), N-cadherin (#13116), HIF1α (#14179), Bcl-XL (#2764), Hsp90 (#4877), FGF2 (#61977), TGFβ (#3711), Cyclin D1 (#2978), GAPDH (#2118), horseradish peroxidase (HRP)-linked rabbit IgG secondary antibody (#7074) and HRP-linked mouse IgG secondary antibody (#7076), from Cell Signaling Technology. Anti-FRS2 (#10425, mouse derived) was purchased from Abcam. AZD4547 was purchased from Selleck Chemistry. The following CyTOF antibodies were purchased from Fluidigm Inc.: STAT3 (#3173003A), pSTAT3 (#3158005A), Vimentin (#3154014A), TGFβ (#3163010B), SOX2 (#3150019B), and Nanog (#3169014A). Custom antibody-metal conjugations were performed using kits purchased from Fluidigm, Inc. (PRD002) and carrier-free antibodies targeting FGFR1 (#9740), pFGFR1 (#3476), pFRS2 Y196 (#3864), and ZEB1 (#3396) were purchased from Cell Signaling Technology.
Ryan M.R., Sohl C.D., Luo B, & Anderson K.S. (2018). The FGFR1 V561M Gatekeeper Mutation Drives AZD4547 Resistance through STAT3 Activation and EMT. Molecular cancer research : MCR, 17(2), 532-543.
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