All the chemicals and solvents were of reagent or better grade purchased from different commercial resources and used without further purification unless mentioned. Powder X-ray diffraction (PXRD) data were recorded on a D5005 Siemens X-ray diffractometer with graphite monochromated Cu Kα radiation (λ = 1.54056 Å) at room temperature (298 K). NMR spectra were recorded on a 300 MHz Bruker Avance 300 FT-NMR spectrometer by calibrating the residual solvent as the reference in DMSO-d6 solution. Thermogravimetric analysis (TGA) was performed under a nitrogen atmosphere with a heating rate of 5°C min−1 on a TA instruments SDT-2960. The C, H, N analysis was carried using an Elementar Vario Micro Cube instrument at the Elemental Analysis Lab, CMMAC, Department of Chemistry, National University of Singapore. The UV irradiation experiments were conducted in a LUZCHEM UV reactor. In the case of the percentage of photo-products formed versus time plots, the ground single crystals were packed between the Pyrex glasses and placed in the UV reactor. These glass slides were flipped back at regular intervals of time to maintain uniform exposure of UV light. The samples were taken out at regular intervals of time and dissolved in DMSO-d6 to record 1H NMR spectra to follow the course of the reaction.
Avance 300 ft nmr spectrometer
Manufactured by Bruker
Sourced in Switzerland, United States
The Avance 300 FT-NMR spectrometer is a laboratory instrument used to perform nuclear magnetic resonance (NMR) spectroscopy. It is designed to analyze the structure and composition of chemical samples through the detection and measurement of nuclear magnetic resonances.
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Lab products found in correlation
Sdt 2960, by TA Instruments (1 mentions)
Micro cube instrument, by Elementar (1 mentions)
D5005, by Siemens (1 mentions)
Vario micro cube instrument, by Elementar (1 mentions)
Ft ir spectrum bx spectrophotometer, by PerkinElmer (1 mentions)
J 810 spectropolarimeter, by Jasco (1 mentions)
Microtof q 2 mass spectrometer, by Bruker (1 mentions)
1020polarimeter, by Jasco (1 mentions)
Jc 1 reagent, by Biotium (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Ft ir 4200, by Jasco (1 mentions)
5 protocols using avance 300 ft nmr spectrometer
1
Characterization of Photochemical Reactions
2
Characterization of Novel Organic Compounds
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Commercially available reagent-grade chemicals were used as received without any further purification unless mentioned. IR spectra were recorded on a FTS165 Bio-Rad FTIR spectrometer by using KBr pellets in the range 4000–400 cm−1. Elemental analysis (C, H and N) was carried out using an Elementar Vario Micro Cube instrument at the Elemental Analysis Lab, CMMAC, Department of Chemistry, National University of Singapore. Thermogravimetric analysis (TGA) was performed under N2 atmosphere with a heating rate of 5°C min−1 on a SDT 2960 Thermal analyzer. NMR spectra were recorded on a 300 MHz Bruker Avance 300 FT-NMR spectrometer by calibrating the residual solvent as the reference in DMSO-d6 solution. The powder X-ray diffraction (PXRD) patterns were recorded on a Siemens D5005 diffractometer with graphite monochromated Cu Kα radiation (λ = 1.54056 Å) at 298 K.
3
Characterization of Organic Compounds
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Melting points were determined using a Griffin melting point apparatus and are uncorrected. Optical rotations at the sodium D line were measured on a JASCO-1020 digital polarimeter. ECD spectra were recorded on a Jasco J-810 spectropolarimeter. IR spectra were measured on a Perkin-Elmer FT-IR Spectrum BX spectrophotometer, with νmax given in cm−1. NMR spectra were measured at 300 MHz (1H) and 75 MHz (13C) on a Bruker AVANCE 300 FT-NMR spectrometer using TMS or residual non-deuterated solvent signals as an internal standard (CDCl3: δH 7.24, δC 77.00; DMSO-d6: δH 2.49 and δC 39.5 for 1H and 13C NMR spectra, respectively). EIMS were recorded on a Thermo Finnigan Polaris Q mass spectrometer at 70 eV (probe). ESIMS were obtained on a Finnigan LC-Q mass spectrometer. The HRESITOFMS were measured on a Bruker micrOTOF-QII mass spectrometer. The X-ray crystallographic data analysis was carried out with a Bruker-Nonius kappaCCD diffractometer with a graphite monochromator, MoKα radiation (λ = 0.71073 Å) at 298(2) K. Silica gel (finer than 0.063 mm, Merck) and Sephadex LH-20 (Pharmacia) were used for column chromatography. TLC analyses were carried out on plates that had been precoated with silica gel F254 from Merck and visualized under a UV light at 254 or 365 nm and by spraying with 5% anisaldehyde-H2SO4 solution followed by heating.
4
Spectroscopic Characterization of Compounds
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1H- and 13C-NMR spectra were recorded on a Bruker Avance 300 FT-NMR spectrometer (Bruker Switzerland AG, Fallanden, Switzerland) operating at 300 MHz (1H) and 75 MHz (13C) using CDCl3 as the solvent. Specific optical rotations were measured using a Jasco-1020 polarimeter (Jasco Corporation, Tokyo, Japan). Column chromatography and TLC were carried out using Merck silica gel 60 (>230 mesh) and precoated silica gel 60 F254 plates, respectively. Spots on the TLC were visualized under UV light (245 or 365 nm) and by spraying with anisaldehyde–H2SO4 reagent followed by heating at 80 °C until maximum color formation occurred. Only analytical grade chemicals were used: JC-1 reagent (Biotium, Biotium, Inc., Fremont, CA, USA), Hoechst 33342 (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA), MTT or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Sigma-Aldrich, St. Louis, MO, USA), fetal bovine serum (FBS), and DMEM (Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA). DMSO and PI (propidium iodide) reagents were purchased from Merck KGaA, St. Louis, MO, USA.
5
Characterization of Organic Compounds
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NMR spectra were recorded on a Bruker AVANCE 300 FT NMR spectrometer (Bruker Corporation, Billerica, MA, USA) operating at 300 MHz for 1 H and at 75 MHz for 13 C using CDCl3 as the solvent. Chemical shifts are reported relative to trimethylsilane (TMS) as the internal standard. Fourier transform infrared (FT-IR) spectra were obtained using a JASCO FT/IR-4200 (JASCO, Hachioji, Japan). UV-vis spectra were recorded on a JASCO V-730iRM spectrometer (JASCO, Hachioji, Japan). Fluorescence spectra were measured on a JASCO FP-6200ST spectrophotometer (JASCO, Hachioji, Japan). All optical measurements were performed at room temperature unless otherwise stated.
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