Gcms qp2010 plus gas chromatograph mass spectrometer

Gas Chromatography Mass Spectrometry (GCMS) analysis of spearmint essential oil (SEO) was performed using a GCMS-QP-2010 Plus Gas chromatograph Mass Spectrometer (Shimadzu, Japan). After injecting the sample, the oven was maintained at a temperature of 50 °C (2 min) and later programmed to 210 °C (ramp rate: 3 °C/min). Further increase in temperature (280 °C) was achieved by increasing the ramp to 6 °C/min. 1 µL of the sample (SEO) was injected (split ratio = 1:115). Helium was utilized at a consistent flow rate (1.0 mL/min) as the carrier gas. The temperatures of the ion source and injector were set at 220 and 260 °C, respectively. The compounds were identified by comparing the retention indices and retention times (RT) of the chromatographic peaks with the reference standards run under identical conditions. Peak enrichment was also performed on co-injection with authentic reference compounds. The comparison of the MS fragmentation pattern with those of pure compounds and mass spectrum database search was performed using the National Institute of Standards and Technology (NIST) MS spectral database (version 2005).

Analysis of the fatty acid profile in the erythrocyte membrane was analyzed as follows: 10-mL blood samples were taken from the antecubital vein 10 min before the physical tests in tubes with tripotassium EDTA (ethylenediamine tetraacetic acid) washed with isotonic saline and purged with nitrogen gas and centrifuged at 2000 rpm for 30 min at 4 °C. The supernatant was collected and frozen at −80 °C and the cell phase (erythrocytes) was frozen at −80 °C for fatty acid analysis. Erythrocyte lipids were determined as methyl esters after methylation reaction, using the method of Lepage and Roy [31 (link)]. GC analysis was performed on a Shimadzu GCMS-QP2010 Plus gas chromatograph/mass spectrometer (Shimadzu, Kyoto, Japan). Fatty acid methyl esters (FAMEs) were identified through mass spectrometry and through comparison of the elution pattern and relative retention times of FAMEs with the reference FAME mixture (GLC-744 Nu-Chek Prep. Inc., Elysian, MN, USA). The results were expressed in relative amounts (percentage molar of total fatty acids) [32 (link)]. The fatty acid methyl esters are analyzed by capillary gas chromatography with an ionized flame detector [33 (link)].