The largest database of trusted experimental protocols

2 protocols using r6 l arginine

1

Proteome Analysis of SIH Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Complete HL-5 and defined SIH (-arginine; -lysine) growth media and unlabelled L-arginine and L-lysine were from Formedium. Labelled K8 L-lysine and R6 L-arginine were from Cambridge Isotope Laboratories. Complete EDTA-free protease inhibitor cocktail was from Roche. Turbo DNase was from Ambion. Novex NuPAGE 4–12% gradient bis-tris gels with MOPS SDS running buffer and Novex colloidal blue staining kit were from Invitrogen. Trypsin Gold was from Promega. LavaPep peptide quantification kit was from Gel Company. ZipTip C-18 peptide purification microcolumns and high fidelity KOD hot start DNA polymerase were from Merck Millipore. Restriction enzymes, T4 DNA ligase and Antarctic phosphatase were from New England Biolabs. Plasmid miniprep kit and PCR clean-up kit were from Qiagen. Krypton fluorescent protein stain and Nunc Lab-Tek chambered cover glass plates for cell imaging were from Thermo Scientific. All the other reagents were obtained from Sigma-Aldrich.
+ Open protocol
+ Expand
2

Quantitative Mass Spectrometry Proteomics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Complete HL-5 and defined SIH (-arginine; -lysine) growth media and unlabelled L-arginine and L-lysine were from Formedium. Labelled K8 L-lysine and R6 L-arginine were from Cambridge Isotope Laboratories. Complete EDTA-free Protease Inhibitors cocktail was from Roche. Turbo DNase was from Ambion. Novex NuPAGE 4-12% gradient bis-tris gels with MOPS SDS running buffer and Novex colloidal blue staining kit were from Invitrogen. Trypsin Gold was from Promega. LavaPep peptide quantification kit was from Gel Company. ZipTip C-18 peptide purification microcolumns and high fidelity KOD hot start DNA polymerase were from Merck Millipore. Restriction enzymes, T4 DNA ligase and Antarctic Phosphatase were from New England Biolabs. Plasmid miniprep kit and PCR clean-up kit were from Qiagen. Krypton fluorescent protein stain and Nunc Lab-Tek chambered cover glass plates for cell imaging were from Thermo Scientific. All the other reagents were obtained from Sigma Aldrich.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!