Spectrum 400 ft ir spectrometer

Philips x-ray diffractometer (PW 1071) with Cuk_α (1.5 Å) radiation having Ni filter was used for x-ray diffraction (XRD) study. All patterns were recorded over the angular range 10 ≤ 2θ/deg ≤ 70 with a step size of ∆2θ = 0.02. The powder samples were ground and dispersed in methanol on a glass slide and allowed to dry. The average crystallite size and strain were calculated using Williamson-Hall model. The lattice parameters were calculated from the least square fitting of the diffraction peaks.
For the characterisation of the nanoparticles, Fourier transform infrared spectroscopy (FTIR) spectra were recorded on a Perkin Elmer Spectrum 400 FT-IR spectrometer. Powder samples were studied by making thin pellets with KBr.
JEOL 2000 FX transmission electron microscope was used for recording TEM images. For the TEM measurement, the powder samples were ground and dispersed in methanol. A drop of the dispersed particles was put over the carbon coated copper grid and evaporated to dryness at room temperature.
All the luminescence spectra were recorded using LS-55 Photoluminescence Spectrometer. Powder samples were dispersed in methanol and spread over the quartz slide and dried at room temperature.

Analytical and preparative TLC was carried out on Merck 60 F254 silica gel plates (absorbent thickness: 0.25 and 0.50 mm, respectively) (Merck, Darmstadt, Germany). Column chromatography (CC) was performed using silica gel 60 (70–230 mesh, ASTM) (Merck, Germany). All solvents were of analytical-grade and were distilled prior to use. IR spectra were recorded using a Perkin-Elmer Spectrum 400 FT-IR Spectrometer. NMR spectra were acquired in CDCl₃ with tetramethylsilane as an internal standard (Merck, Germany) using either a JOEL ECX 500 MHz NMR Spectrometer or a BRUKER Advance III 600 NMR Spectrometer (BRUKER, Billerica, MA, USA). LCMS-IT-TOF spectra were obtained using an Agilent 6530 Accurate-Mass Q-TOF LC/MS system (Agilent, Santa Clara, CA, USA). UV spectra were recorded using a Shimadzu UV-250 UV-Vis Spectrophotometer (Shimadzu, Tokyo, Janpan). A Jasco P-1020 polarimeter was used to record the optical rotation (JASCO, Hachioji, Tokyo, Japan).

The morphology of the fibermat was investigated with two SEM microscopes, namely Carl Zeiss EVO MA10 and FEI Nova NanoSEM450, with a 5–30 kV impinging voltage. Transmission electron microscopic images were obtained using Phillips CM100 TEM (Nashville, TN, USA) under an accelerating voltage of 100 kV. The chemical analysis of the samples was done by X-ray photoelectron spectroscopy (XPS), obtained using PHI Quantera SXM using monochromatic Al-Kα with an energy of 1486.6 eV. The Raman imaging system of Horiba Jobin Yvon LabRam Aramis was utilized using a He-Ne laser (wavelength of 633 nm and power of 17 mW) to collect the Raman spectra of the pyrolyzed samples. The FTIR spectra of the fibermats were collected utilizing the Spectrum 400 FT-IR spectrometer from PerkinElmer (Waltham, MA, USA).

Column chromatography (CC) was run on a silica gel 60 column (40–63 μm particle size, Merck, Darmstadt, Germany). Thin layer chromatography (TLC) was performed on an aluminum supported silica gel 60 F₂₅₄ column (Merck). Preparative TLC (PTLC) was run on glass coated with silica gel 60 F₂₅₄ (Merck). ¹H NMR and ¹³C NMR spectra were analyzed in CDCl₃ on a JEOL JNM-FX500 spectrometer (Tokyo, Japan). The ultraviolet absorption spectra were obtained on a Shimadzu UV-160A spectrophotometer (Kyoto, Japan) using methanol (CH₃OH) as a solvent. The separation was performed on a HPLC machine (Gilson, Inc., Middleton, WI, USA) with a photodiode array (PDA) detector and an ODS C₁₈ column (Phenomenex, Torrance, CA, USA). The mass spectra were measured with an Agilent 6530 mass spectrometer (Santa Clara, CA, USA). The infrared spectra were obtained on a Perkin Elmer Spectrum 400-FTIR spectrometer (Waltham, MA, USA) with CHCl₃ as a solvent.

The peptide samples (0.2 mM) were prepared in 0.4 mM sodium bicarbonate buffer, pH 11 and incubated at 37°C with continuous shaking for 5 days. The FTIR spectra of the peptide samples were recorded in the range of 4,000–400 cm⁻¹, using Spectrum 400 FTIR spectrometer [PerkinElmer equipped with UATR (Universal Attenuated Total Reflectance)] and DTGS detector.

UV and ECD data were
obtained using a Chirascan V100 instrument with a 1.0 cm quartz cuvette.
Optical rotations were recorded with an Autopol V polarimeter equipped
with a 10 cm cell at 20 °C and at the Na D line at 589.3 nm.
NMR experiments were performed on a 600 MHz spectrometer and a Varian
Inova 500 MHz spectrometer. Chemical shifts were referenced to the
residual solvent signals in ppm (CD₃OD, at δ_H 3.31 and δ_C 49.00 ppm). High-resolution
mass spectra were obtained with an Agilent 6540 Q-TOF mass spectrometer
equipped with an Agilent 1290 UHPLC and autosampler. Preparative and
semipreparative purifications were carried out on a Jasco LC-2000
series HPLC system. Optical rotations were recorded on a Unipol L1000
polarimeter at the sodium D-line (589.3 nm) with a 10 cm cell at 20
°C. IR spectra were recorded on a PerkinElmer Spectrum 400 FT-IR
spectrometer (4000–650 cm^–1).

Column chromatography was performed using silica gel 60, 230–400 mesh ASTM (Merck, 0.040–0.063mm). Aluminium supported silica gel 60 F₂₅₄ plates 20 x 20cm (absorbent thickness: 0.25 mm) were used for thin layer chromatography (TLC) (Merck, Germany). Preparative thin layer chromatography (PTLC) (Merck, Germany) silica gel 60 F₂₅₄ glass plates 20 x 20cm (absorbent thickness: 0.50 mm) were used for separation of compounds. IR spectrum was recorded using a Perkin-Elmer Spectrum 400 FT-IR Spectrometer with spectroscopic grade chloroform as the solvent. 1D- and 2D-NMR spectra were recorded in chloroform CDCl₃ (Merck, Germany) using JEOL ECA 400 MHz NMR spectrometer. The LCMS-IT-TOF spectra were recorded on a UFLC Shimadzu Liquid Chromatography with a SPD-M20A diode array detector coupled to a IT-TOF mass spectrometer. UV spectra were recorded using a Shimadzu 1650 PC UV-Vis Spectrophotometer with spectroscopic grade methanol (CH₃OH) as solvent. All solvents were of analytical grade and were distilled prior to use.