Tiangen genomic dna extraction kit
The TIANGEN Genomic DNA Extraction Kit is a laboratory tool designed for the efficient extraction and purification of genomic DNA from a variety of biological samples. It provides a straightforward and reliable method to obtain high-quality DNA for various downstream applications, such as PCR, sequencing, and genetic analysis.
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Chromatin Immunoprecipitation Assay in HEK293T Cells
DNA and RNA Isolation and Real-Time qPCR DNA was isolated using the TIANGEN Genomic DNA Extraction Kit (catalog no. DP304; TIANGEN Biotech, Beijing, China). RNA was isolated with TRIzol reagent (Invitrogen), and 5 mg of total RNA per reaction was used to synthesize the cDNA with the SuperScripts II First-Strand cDNA Synthesis Kit (Invitrogen). Real-time qPCR was performed with the QuantiTect SYBR Green PCR Kit (QIAGEN, Dusseldorf, Germany) by the CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA). Samples were tested in a 96-well format in triplicate. For experiments in the SGBS cells, GAPDH was used as an endogenous control.
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