Male cd 1 retired breeder mice

Male C57BL/6J mice (7–9 weeks old) were obtained from Jackson Laboratory and housed on a 12 h light-dark cycle with access to food and water ad libitum. Male CD1 retired breeder mice (9–13 months old) were obtained from Charles River Laboratories. Mice acclimated to the facility for 1 week before any experimentation. D1-Cre hemizygote (line FK150) or D2-Cre hemizygote (line ER44) BAC transgenic mice from GENSAT [13 ] on a C57BL/6J background were used for behavioral experiments. To induce Rpl22-HA in either D1 or D2 neurons, we crossed homozygous RT mice [8 (link)] with D1 or D2 heterozygous mice. To label D1 neurons for electrophysiological recordings, Ai6 mice were crossed with D1-Cre mice (Ai6; D1-Cre). All behavioral tests and animal sacrifices (tissue collections and acute brain slice preparations) were performed at ZT 02-06. All animal procedures were approved by the University of Arizona Medical School Institutional Animal Care and Use Committees.

Experimental procedures were performed in accordance with the guidelines of the Canadian Council of Animal Care and approved by the McGill University and Douglas Hospital Animal Care Committee. All mice were housed in a temperature-controlled and humidity-controlled (21–22°C; 60%) colony room of the Neurophenotyping center of the Douglas Mental Health University Institute, on a 12/12 h light/dark cycle (light on at 8 A.M.). The mice had ad libitum access to food and water throughout the experiments (except during food restriction for Go/No-Go experiments). Mice were assigned randomly to each experimental condition.
Male C57BL/6J wild-type mice (n = 27) supplied by The Jackson Laboratory, arrived at the housing facilities on postnatal day (PND)24. These mice were housed in groups of three to four animals per cage before exposure to AcSD and single-housed after AcSD. Male CD-1 retired breeder mice (more than three months of age) obtained from Charles River Canada were used as aggressors in the AcSD paradigm.
The present study is aimed primarily at validating the custom-built behavioral and imaging setup, so only male mice were used as subjects. Although sex differences in the effects of adolescent social stress are very likely and of considerable interest, they are outside the scope of the present article.