Plasma was centrifuged at 1000× g at 4 °C for 10 min and stored at −80 °C. The levels of TG, LDL, HDL, and total cholesterol were measured using commercial kits (Roche Molecular Biochemical, Pleasanton, CA, USA). Moreover, the plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assayed by the Reflovet Plus equipment (Roche, Basel, Switzerland) using commercial kits: Reflotron GOT, Reflotron GPT (Roche, Basel, Switzerland). TG levels in the liver were assayed using the Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI, USA), according to the manufacturer’s instructions.
Reflotron got
Manufactured by Roche
Sourced in Switzerland
The Reflotron GOT is a compact, fully automated diagnostic instrument designed for the quantitative determination of glutamic oxaloacetic transaminase (GOT) levels in whole blood, plasma, or serum samples. It provides quick and reliable results, making it a useful tool for clinical laboratories and healthcare settings.
Automatically generated - may contain errors
Lab products found in correlation
Reflotron gpt, by Roche (5 mentions)
Triglyceride colorimetric assay kit, by Cayman Chemical (4 mentions)
Reflovet plus, by Roche (2 mentions)
Reflovet plus equipment, by Roche (2 mentions)
Reflotron alp, by Roche (1 mentions)
Reflotron ggt, by Roche (1 mentions)
Reflotron bil, by Roche (1 mentions)
5 protocols using reflotron got
1
Plasma Lipid and Enzyme Analysis
2
Plasma Biomarkers and Hepatic Lipids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was centrifuged at 1000 × g and 4 °C for 10 min. Plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were evaluated using commercially available kits Reflotron GPT and Reflotron GOT (Roche, Basel, Switzerland) by the Reflovet Plus analyser (Roche, Basel, Switzerland). The content of triglycerides in the liver was measured using the Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI, USA), according to the manufacturer’s instructions.
3
Biochemical Profiling of Blood and Liver
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The blood was collected from the right ventricle. Plasma was separated by centrifugation at 1000g at 4°C for 10 minutes and stored in −80°C until assayed. Total cholesterol, level of high‐density lipoproteins (HDLs) and low‐density lipoproteins (LDLs), as well as triglycerides (TGs) were assayed using commercially available kits (Roche Molecular Biochemical, Pleasanton, CA). In addition, levels of some inflammation markers, such as interleukin 6 (IL)‐6), IL‐12, vascular cell adhesion protein 1 (VCAM‐1), and monocyte chemoattractant protein 1 (MCP‐1), were measured by ELISA using commercially available kits (R&D Systems, Minneapolis, MN). Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) plasma levels were assayed using commercially available kits: Reflotron GPT, Reflotron GOT (Roche, Mannheim, Germany), and Reflovet Plus equipment (Roche, Germany).
Apart from that, the level of TGs in the liver was measured using Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI), according to the manufacturer's instructions.
Apart from that, the level of TGs in the liver was measured using Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI), according to the manufacturer's instructions.
4
Lipid and Enzyme Profile Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The blood was centrifuged for 10 min, 1000× g at 4 °C and the plasma was harvested and stored in -80 °C until use. The levels of total cholesterol (TC), triglycerides (TG), and low- and high-density lipoproteins (LDL and HDL) were measured using an enzymatic method on a Cobas 8000 analyzer (Roche Diagnostics, Indianapolis, IN, USA). In addition, plasmatic levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined by commercially available kits: Reflotron GPT and Reflotron GOT (Roche, Mannheim, Germany), and Reflovet Plus equipment (Roche, Mannheim, Germany).
TG levels in the liver were quantified using the Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI, USA), according to the manufacturer’s protocol.
TG levels in the liver were quantified using the Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI, USA), according to the manufacturer’s protocol.
5
Serum Biochemical Profiling in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats serum samples were subjected to biochemical analyses for the levels of serum alanine aminotransaminase (ALT; Reflotron GPT, Roche Diagnostics GmbH, Mannheim, Germany), spartate aminotransaminase (AST; Reflotron GOT, Roche Diagnostics GmbH, Mannheim, Germany), alkaline phosphatase (ALP; Reflotron ALP, Roche Diagnostics GmbH, Mannheim, Germany), γ-glutamyl transferase (GGT; Reflotron GGT, Roche Diagnostics GmbH, Mannheim, Germany) and bilirubin (BIL; Reflotron BIL, Roche Diagnostics GmbH, Mannheim, Germany), using Reflotron Plus Analyzer (Woodley Equipment Co., Ltd., UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!