GUCY2C-expressing mouse (BALB/c) CT26 colorectal cancer cells were used for in vivo tumor studies.17 (link) Luciferase-expressing cells were generated by transduction with lentiviral supernatants produced by 293FT cells (Invitrogen) with pLenti4-V5-GW-luciferase.28 (link) For tumor experiments, BALB/cJ mice were immunized with 1010 vp of Ad5-GUCY2C-S1, Ad5.F35-GUCY2C-S1, or PBS (control) 7 days before delivering 5×105 CT26 cells into tail veins. Tumor burden was quantified weekly by subcutaneous injection of 3.75 mg of D-luciferin potassium salt (Gold Biotechnologies) in PBS followed by an 8 min incubation and imaging with a 10 s exposure using a Caliper IVIS Lumina XR imaging station (PerkinElmer). Total radiance (photons/second) was measured using Living Image In Vivo Imaging Software (PerkinElmer).
Caliper ivis lumina xr imaging station
Manufactured by PerkinElmer
The Caliper IVIS Lumina XR imaging station is an in vivo imaging system designed for non-invasive, real-time visualization and quantification of bioluminescent and fluorescent signals in small animal models. The system utilizes sensitive charge-coupled device (CCD) cameras and advanced optics to capture high-resolution images and videos of biological processes occurring within the living subject.
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3 protocols using caliper ivis lumina xr imaging station
1
In Vivo Tumor Studies with GUCY2C-expressing Cells
2
Murine Colorectal Cancer Metastasis Model
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The murine CT26 colorectal cancer cell line expressing mouse GUCY2C and luciferase (23 (link)) was used for in vivo tumor studies. Seven days after final immunizations, mice received 5x105 CT26 cells via i.v. tail vein injection to model metastatic colorectal cancer recurrence in the lungs. Tumor burden was quantified by subcutaneous injection of 3.75 mg of D-luciferin potassium salt (Gold Biotechnologies) in PBS solution. Following an 8 min incubation, mice were imaged with a ten-second exposure using a Caliper IVIS Lumina XR imaging station (PerkinElmer). Total radiance (photons/second) was quantified by Living Image In Vivo Imaging Software (PerkinElmer).
3
Colorectal Cancer Cell Line for In Vivo Studies
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The BALB/cJ CT26 colorectal cancer cell line expressing GUCY2C and luciferase19 (link) was used for in vivo tumor studies. For most studies, six days after final immunizations, mice received 5 × 105 CT26 cells via intravenous (i.v.) tail vein injection. However, for memory studies, tumor challenges occurred 63 days after the final immunization. Tumor burden was quantified by subcutaneous injection of 3.75 mg of D-luciferin potassium salt (Gold Biotechnologies) in PBS followed by an eight-minute incubation and imaging with a ten-second exposure using a Caliper IVIS Lumina XR imaging station (PerkinElmer). Total radiance (photons/second) was measured using Living Image In Vivo Imaging Software (PerkinElmer).
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