Reversed phase x select hss t3 column

HPLC separation was accomplished using a (Waters) reversed-phase X select HSS T3 column (2.1×150 mm, 2.5 µm), a (Phenomenex) precolumn, and in-Line filter disks (0.5 µm × 3.0 mm). Adopting conditions described by [17 (link)]. PeakView^TM software was used to compare retention time and m/z values obtained by MS and MS² to identify compounds. PeakView^TM software’s XIC Manager was used to calculate peak area values. For each targeted analyte, extracted ion chromatograms (XICs) were automatically generated and compared to a user-defined threshold [51 (link)]. The LC-MS/MS analysis was carried out in Proteomics and Metabolomics Unit, Children’s Cancer Hospital (57357).

Compounds in the APEE were detected by Proteomics and Metabolomics Unit, Children’s Cancer Hospital (57357), Basic Research Department, Cairo, Egypt. Adopting the criteria described by Attallah et al. [51 (link)]. For HPLC separation, A (Waters) reversed-phase X select HSS T3 column (diameters are 2.1 × 150 mm, 2.5 μm), a (Phenomenex) precolumn, and in-Line filter discs (0.5 μm × 3.0 mm) were employed. To identify compounds, PeakView^TM software was used to compare retention duration and m/z values obtained by MS and MS². The XIC Manager in PeakView^TM software was used to calculate peak area values. Extracted ion chromatograms (XICs) for each targeted analyte were automatically created and compared to a user-defined threshold [51 (link)].