Western blotting was performed from neonatal rat cardiomyocytes using standard procedures. Antibodies used were METTL3 (Bethyl Laboratories), MAP3K6 (Novus Biologicals), MAP4K5 (Thermo Scientific Pierce), MAPK14 (Cell Signaling Technology) and GAPDH (Fitzgerald Industries). Masson’s trichrome staining was performed from histological sections generated from paraffin-embedded hearts. Immunostaining was performed using α-Actinin antibodies (Sigma Aldrich) and cell area was quantified using CellProfiler following published methods.24 (link) Detection of the cell membrane was performed using FITC-conjugated Wheat Germ Agglutinin (Sigma Aldrich). Cross-sectional area was measured using ImageJ. RNA was extracted from neonatal rat cardiomyocytes or mouse hearts using Trizol and reverse transcription was performed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Selected genes and m6A peaks were analyzed by real-time PCR using SYBR green (Applied Biosystems). Quantified mRNA expression was normalized to Rpl7 (Ribosomal Protein L7), and expressed relative to controls.
Map3k6
Manufactured by Novus Biologicals
MAP3K6 is a protein kinase that is involved in the mitogen-activated protein kinase (MAPK) signaling pathway. It plays a role in the activation of the extracellular signal-regulated kinase (ERK) cascade. MAP3K6 is a key component of the signaling network that regulates cellular processes such as proliferation, differentiation, and stress response.
Automatically generated - may contain errors
Lab products found in correlation
Sybr green, by Thermo Fisher Scientific (2 mentions)
Mettl3, by Fortis Life Sciences (2 mentions)
Mapk14, by Cell Signaling Technology (2 mentions)
α actinin antibody, by Merck Group (2 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Map4k5, by Thermo Fisher Scientific (2 mentions)
Gapdh, by Biosynth (2 mentions)
Fitc conjugated wheat germ agglutinin, by Merck Group (1 mentions)
Fluorescein isothiocyanate conjugated wheat germ agglutinin, by Merck Group (1 mentions)
2 protocols using map3k6
1
Western Blotting and Immunostaining of Neonatal Rat Cardiomyocytes
2
Cardiac Protein and Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting was performed from neonatal rat cardiomyocytes using standard procedures. Antibodies used were METTL3 (Bethyl Laboratories), MAP3K6 (Novus Biologicals), MAP4K5 (Thermo Scientific Pierce), MAPK14 (Cell Signaling Technology), and GAPDH (Fitzgerald Industries). Masson trichrome staining was performed from histological sections generated from paraffin-embedded hearts. Immunostaining was performed using α-actinin antibodies (Sigma-Aldrich), and cell area was quantified using CellProfiler following published methods.24 (link) Detection of the cell membrane was performed using fluorescein isothiocyanate–conjugated wheat germ agglutinin (Sigma-Aldrich). The cross-sectional area was measured using ImageJ. RNA was extracted from neonatal rat cardiomyocytes or mouse hearts using Trizol, and reverse transcription was performed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Selected genes and m6A peaks were analyzed by real-time polymerase chain reaction using SYBR green (Applied Biosystems). Quantified mRNA expression was normalized to Rpl7 (ribosomal protein L7) and expressed relative to controls.
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