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Bcat2 antibody

Manufactured by Cell Signaling Technology

The BCAT2 antibody is a laboratory reagent used to detect the presence and quantify the expression levels of the BCAT2 protein in biological samples. BCAT2 is an enzyme involved in the metabolism of branched-chain amino acids. The antibody can be used in various analytical techniques, such as western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of BCAT2 in cells and tissues.

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2 protocols using bcat2 antibody

1

Mitochondrial Protein Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the chemicals were obtained from Sigma-Aldrich unless otherwise specified. Following antibodies were used in this study: UCP1 antibody (ab-10983, Abcam), BCAT1 antibody (TA504360, OriGene), BCAT2 antibody (9432, Cell Signaling Tech), BCKDHA antibody (sc-271538, Santa Cruz), TOM20 antibody (11802–1-AP, Proteintech), COX-IV antibody (4850, Cell Signaling), OXPHOS cocktail (Abcam, ab110413), PDH-E1α antibody (sc-377092, Santa Cruz), PDH-E1α (pSer232) antibody (AP1063, Millipore), PDH-E1α (pSer293) antibody (ab177461, Abcam), PDH-E1α (pSer300) antibody (AP1064, Millipore), GAPDH antibody (sc-32233, Santa Cruz), and β-actin antibody (A3854, Sigma-Aldrich). Polyclonal antibody for SLC25A44 was generated by using amino acids (MEDKRNIQIIEWEHLDKKKC, MMQRKGEKMGRFQVC, and CKKLSLRPELVDSRH) as epitopes for immunization in rabbit (GeneScript).
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2

Mitochondrial Protein Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the chemicals were obtained from Sigma-Aldrich unless otherwise specified. Following antibodies were used in this study: UCP1 antibody (ab-10983, Abcam), BCAT1 antibody (TA504360, OriGene), BCAT2 antibody (9432, Cell Signaling Tech), BCKDHA antibody (sc-271538, Santa Cruz), TOM20 antibody (11802–1-AP, Proteintech), COX-IV antibody (4850, Cell Signaling), OXPHOS cocktail (Abcam, ab110413), PDH-E1α antibody (sc-377092, Santa Cruz), PDH-E1α (pSer232) antibody (AP1063, Millipore), PDH-E1α (pSer293) antibody (ab177461, Abcam), PDH-E1α (pSer300) antibody (AP1064, Millipore), GAPDH antibody (sc-32233, Santa Cruz), and β-actin antibody (A3854, Sigma-Aldrich). Polyclonal antibody for SLC25A44 was generated by using amino acids (MEDKRNIQIIEWEHLDKKKC, MMQRKGEKMGRFQVC, and CKKLSLRPELVDSRH) as epitopes for immunization in rabbit (GeneScript).
+ Open protocol
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