Mk2206 s1078

Manufactured by Selleck Chemicals

Sourced in United States

MK2206 (S1078) is a lab equipment product manufactured by Selleck Chemicals. It is a small molecule that functions as an allosteric inhibitor of the serine/threonine protein kinase AKT. The core function of this product is to regulate the activity of the AKT signaling pathway.

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S1078 (8 citations)

8 protocols using mk2206 s1078

Investigating ACTL6A and FSHR in AKT Signaling

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ACTL6A (ab131272) and FSH receptor (FSHR) (ab75200) antibodies were from Abcam (Cambridge, MA, USA). ACTL6A (sc-137062) mouse monoclonal antibody was from Santa Cruz (Dallas, TX, USA). AKT (#9272) and Phospho-AKT (Ser473) (#4060) antibodies were from Cell Signaling Technology (Danvers, MA, USA). PGK1 (17811-1-AP), GAPDH (10494-1-AP), and secondary antibodies were from Proteintech (Wuhan, China). FSH (869001) was from Merck (Darmstadt, Germany). LY294002 (S1105) and MK2206 (S1078) were from Selleck (Houston, USA). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) kit (C0009) was obtained from Beyotime Biotech, Inc. (Shanghai, China). Transwell® Polycarbonate Membrane (#3422) was obtained from Corning (NY, USA).

Zhang J., Zhang J., Wei Y., Li Q, & Wang Q. (2019). ACTL6A regulates follicle-stimulating hormone-driven glycolysis in ovarian cancer cells via PGK1. Cell Death & Disease, 10(11), 811.

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Metabolic Profiling of Cellular Signaling

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GLUT-1 (ab652) and H3K27Ac (ab4729) were purchased from Abcam. HK (A0994) and horseradish peroxidase (HRP)-goat anti-Rabbit were purchased from ABclonal. HRP-conjugate anti-mouse (w402b) was purchased from Promega. H2BK (07-373), H4K (06-866), and H3K (06-599) were purchased from Millipore. Flag (F7425) was purchased from Sigma-Aldrich. All other antibodies including Rictor (2140), PRAS40 (2691), AKT (9272), GSK3b (9315), ACC (3676), ACLY (4332), FASN (3180), PPARg (2443), ACSS2 (3658), S6 (2217), S473-AKT (4058), T308-AKT (4056), S455-ACLY (4331), T246-pPRAS40 (2997), HA-tag (2367), S9-GSK3b (9315), S235/236-S6 (4858), H3 (9715), H3K9Ac (9649), H3K14Ac (7627), and a-Tubulin (2125) were purchased from Cell Signaling Technologies. 4-OHT was obtained from Toronto Research Chemicals. D-[U-¹⁴C]-glucose (NEC042V250UC) was purchased from Perkin Elmer. MK2206 (S1078) was purchased from Selleck Chemicals. T3 (T2877), dexamethasone (D1756), IBMX (I5879), and insulin (I2643) were from Sigma-Aldrich. [U-¹³C] glucose (CLM-1396-1) and [1,2-¹³C] acetate (CLM-440-PK) were purchased from Cambridge Isotope Laboratories, Inc.

Martinez Calejman C., Trefely S., Entwisle S.W., Luciano A., Jung S.M., Hsiao W., Torres A., Hung C.M., Li H., Snyder N.W., Villén J., Wellen K.E, & Guertin D.A. (2020). mTORC2-AKT signaling to ATP-citrate lyase drives brown adipogenesis and de novo lipogenesis. Nature Communications, 11, 575.

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Cancer Cell Lines and Reagents

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Lung (NCI-H23, A549, NCI-H358, Calu-1, NCI-H460, and NCI-H1299), breast (SK-BR-3, MCF-7, and MDA-MB-231), colorectal (HCT116), hepatocellular (SK-HEP-1), and cervical (HeLa) cancer cells were obtained from the Korean Cell Line Bank (Seoul, Korea). Melanoma cell lines (A375 and A2058) were obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in 10% fetal bovine serum (FBS) as well as penicillin/streptomycin-contained Dulbecco’s modified Eagle’s medium (DMEM) and Roswell Park Memorial Institute (RPMI) 1640 at both 20% O2 and 5% CO2. Cell culture medium, FBS, and antibiotics were purchased from HyClone Thermo Scientific (Waltham, MA, USA). Simvastatin (S6196), Lovastatin (438185), Lonafarnib (SML1457), and GGTI-2133 (G5294) were purchased from Sigma Aldrich (St. Louis, MO, USA). Atorvastatin (S5715), Fluvastatin (S2061), and MK-2206 (S1078) were obtained from Selleckchem (Houston, TX, USA). All chemicals stock solution was dissolved in dimethyl sulfoxide (DMSO).

Lim W.J., Lee M., Oh Y., Fang X.Q., Lee S., Lim C.H., Park J, & Lim J.H. (2021). Statins Decrease Programmed Death-Ligand 1 (PD-L1) by Inhibiting AKT and β-Catenin Signaling. Cells, 10(9), 2488.

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Antibody and Inhibitor Utilization Protocols

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Primary antibodies used in this study are summarized in Supplementary Table S1. Anti-HIF-1α previously made were used as described^{41 (link)}. MLN4924 was synthesized, as described^{11 (link)}. LY294002 (L9908) (a PI3K inhibitor), Rapamycin (mTOR inhibitor) and MK-2206 (s1078) (an AKT inhibitor), (Selleck Chemicals, Houston, TX, USA) were purchased from the indicated companies. Actinomycin D (A1410) was purchased from Sigma Aldrich. CHX (C1988) was purchased from Sigma Aldrich.

Park J.B., Seo J., Park J.W, & Chun Y.S. (2020). Neddylation blockade induces HIF-1α driven cancer cell migration via upregulation of ZEB1. Scientific Reports, 10, 18210.

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Mitochondrial Dynamics Modulation Protocol

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Doxycycline (D9891) and N-acetylcysteine (NAC, A9165) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-(2,4-dichloro-5-methoxyphenyl)-2,3-dihydro-2-thioxo-4(1 H)-quinazolinone (Mdivi-1, BML-CM127) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). MK-2206 (S1078) was purchased from Selleck Chemicals (Houston, TX, USA). For gene expression knockdown, cells were transfected with validated small inhibitory RNAs (siRNAs) targeting human HSPA9 (5′-AAACGCAAGUGGAAAUUAA-3′), Drp1 (5′-GAGGUUAUUGAACGACUCA-3′), or IFT88 (5′-CCGAAGCACUUAACACUUA-3′) using Lipofectamine 2000. The siRNAs were synthesized by Genolution (Seoul, Korea). At 48 h post-transfection, the cells were treated with the indicated reagents.

Bae J.E., Jang S., Kim J.B., Hyung H., Park N.Y., Kim Y.H., Kim S.H., Kim S.H., Ha J.M., Oh G.S., Park K., Jeong K., Jang J.S., Jo D.S., Kim P., Lee H.S., Ryoo Z.Y, & Cho D.H. (2023). Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells. Molecular Brain, 16, 41.

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Investigating Cellular Signaling Pathways

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Slug (9585S), CUL1 (4995S), NEDD8 (2745S), p-AKT (ser473) (9271S), phospho-mTOR (ser2448) (2971S), E-Cadherin (3195S), Phospho-STAT3 (9145S), Phospho-Smad2/3 (8828S), Phospho-ERK (9101S), and antibodies of EMT markers (ZO-1, Vimentin, ZEB1, N-cadherin, Claudin-1, β-Catenin, Snail) (Epithelial–Mesenchymal Transition (EMT) Antibody Sampler Kit 9782T) were purchased form Cell Signaling Technology (Danvers, MA); β-tubulin (sc-166729), p53 (sc-126), p21 (sc-6246), MDM2 (sc-965), and Ubiquitin (sc-8017) antibodies from Santa Cruz Biotechnology (Dallas, TX). MLN4924 was synthesized, as previously described [26 (link)]. MG132 (BML-PI102) (Enzo Life Sciences, Plymouth Meeting, PA, USA), Cycloheximide (C1299) (Sigma Aldrich, St. Louis, MO, USA), LY294002 (L9908), MK-2206 (s1078), and Rapamycin (s1039) (Selleck Chemicals, Houston, TX, USA) were purchased from the indicated company.

Kim Y., Park J.B., Fukuda J., Watanabe M, & Chun Y.S. (2021). The Effect of Neddylation Blockade on Slug-Dependent Cancer Cell Migration Is Regulated by p53 Mutation Status. Cancers, 13(3), 531.

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NSCLC and HEK293T Cell Culture

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Human non-small cell lung cancer (NSCLC) H1299 and HEK 293T (human embryonic kidney) cells were cultured in DMEM (Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS; HyClone, Logan, UT, USA). Human NSCLC H1975 cells were cultured in RPMI-1640 medium (Gibco) supplemented with 10% FBS (HyClone). All cells were grown in medium supplemented with 100 units/mL penicillin (Gibco) and 100 μg/mL streptomycin (Gibco). Cells were maintained in a humidified 37 °C incubator under a 5% CO₂ atmosphere. Cells at 75-85% confluence were treated with the indicated chemical compound. MK2206 (S1078) was purchased from Selleck Chemicals (Houston, USA).

Lv T., Lv H., Fei J., Xie Y., Lian D., Hu J., Tang L., Shi X., Wang J., Zhang S., Li F., Jiang X, & Yi Y. (2020). p53-R273H promotes cancer cell migration via upregulation of neuraminidase-1. Journal of Cancer, 11(23), 6874-6882.

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Inhibition of Tubulin and HO-1 Signaling

Cited 1 time

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MK-2206 (S1078) and SC79 (S7863) were from Selleck (Shanghai, China). The anti-β-tubulin antibody (SAB4500088) and the heme oxygenase-1 (HO-1) inhibitor Zinc protoporphyrin (ZnPP, 282820) were obtained from Sigma (St. Louis, MO). All other antibodies were from Cell Signaling Tech (Nanjing, China) and Santa Cruz Biotech (Shanghai, China) as described [6 (link), 10 (link)].

Gong Y.Q., Huang W., Li K.R., Liu Y.Y., Cao G.F., Cao C, & Jiang Q. (2016). SC79 protects retinal pigment epithelium cells from UV radiation via activating Akt-Nrf2 signaling. Oncotarget, 7(37), 60123-60132.

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