Whole-cell lysates were prepared with M-PER Mammalian Protein Extract Reagent (Thermo Scientific). Cell lysates or EV suspensions were denatured in 4× sample buffer solution without 2-Mercaptoethanol (Wako Pure Chemical Industries). Signals were detected using ImmunoStar LD (Wako Pure Chemical Industries). Western blots were performed as described previously [27 ] using antibodies directed to CD9 (sc-59140; Santa Cruz Biotechnology), CD63 (556019; BD Biosciences), GFP (M048-3; MBL), L1TD1 (6439; ProSci), L1-ORF1p (MABC1152; Merck), L1-ORF2p (sc-67197; Santa Cruz Biotechnology), APOBEC3B (ab184990; Abcam), APOBEC3C (GTX102164; GeneTex), APOBEC3F (ab227962; Abcam) or GAPDH (MAB374; Millipore). Uncropped blots are shown in Figures S7, S8, and S9.
Mabc1152
Manufactured by Merck Group
Sourced in Germany
MABC1152 is a laboratory equipment manufactured by Merck Group. It is designed to perform a specific function within the research and development process. The core function of MABC1152 is to facilitate a particular aspect of laboratory analysis, but no further details on its intended use or applications are provided.
Automatically generated - may contain errors
Lab products found in correlation
M per mammalian protein extract reagent, by Thermo Fisher Scientific (1 mentions)
Mab374, by Merck Group (1 mentions)
2 mercaptoethanol, by Fujifilm (1 mentions)
Ab184990, by Abcam (1 mentions)
Ab227962, by Abcam (1 mentions)
Sc 59140, by Santa Cruz Biotechnology (1 mentions)
Discovery xt system, by Roche (1 mentions)
Ab150080, by Abcam (1 mentions)
3 protocols using mabc1152
1
Immunoblotting of Extracellular Vesicles
2
Immunohistochemical Localization of L1orf1p
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L1orf1p IHC was performed on a Ventana Discovery XT system, using standard protocol. In short, antigen retrieval was performed using Discovery CC1 buffer (Roche Diagnostics GmbH, Mannheim, Germany 06414575001 (950-500)), followed by incubation with the primary antibody against L1orf1p (1:2000, Mouse Monoclonal, MABC1152, Merck, Darmstadt, Germany), followed by anti-mouse-HRP secondary antibody (Roche 05266556001 (760-150)). An expert liver pathologist assessed the staining.
3
Immunocytochemistry of H4K16ac and L1 ORF1
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Cells were grown on 24-well cell culture plates, fixed with 4% formaldehyde, incubated for 5 min with permeabilization buffer (PBS containing 0.1% Triton X-100), and blocked with PBS containing 0.1% Triton X-100 and 2% BSA) for 1 h. Primary antibodies to H4K16ac (Abcam, Ab109463, 1:500) and L1 ORF1 (Merck Millipore, MABC1152, 1:500 dilution) were added overnight at 4 °C, washed three times with PBS (10 min each) and incubated with anti-rabbit secondary antibodies (Abcam, Ab150080, 1:500) and DAPI (1:1000). After washing 3 times with PBS (10 min each), the cells were left in PBS and imaged with Incell2000.
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