Polyallylamine

Agarose microbeads (50–150 μm
diameter) were purchased from Agarose Bead Technologies (Madrid, Spain).
Polyethyleneimine (PEI) solution in H₂O (M_w ∼ 60 000, 50 wt %), polyallylamine (PAH)
solution in H₂O (M_w ∼
65 000, 10 wt %), polydiallyldimethylammonium chloride (PDADMAC)
solution in H₂O (M_w < 100 000,
35 wt %), pyridoxal 5′-phosphate hydrate (PLP, 98%), rhodamine
B isothiocyanate mixed isomers (RhB), acetone, 2-phenylethylamine
(PEA, 98%), iminodiacetic acid (IDA), albumin bovine serum standard
(BSA), and other reagents and solvents of analytical grade were purchased
from Sigma-Aldrich (St. Louis, IL). Nicotinamide adenine dinucleotide-reduced
sodium salt (NADH) and flavin mononucleotide sodium salt (FMN) were
purchased from GERBU Biotechnik GmbH (Heidelberg, Germany). Flavin
Adenine dinucleotide disodium salt hydrate (FAD, 94%) was purchased
from Cymit Quimica S.L. (Barcelona, Spain). Riboflavin (Rf, 98%) was
purchased from Acros Organics B.V.B.A. (Fair Lawn, New Jersey, United
States). Isopropyl-β-d-thiogalactopiranoside (IPTG,
100%) was purchased from Fisher Bioreagents. The Bradford protein
assay dye reagent was purchased from BIORAD (Biorad. Hercules, CA).
Clear bottom black and white microplates (96-well) were purchased
from Avantor (2021 VWR International, LLC). μ-Slides 8 well
glass bottom was purchased from Ibidi (Planegg, Germany).

Agarose 10BCL was purchased from Agarose Beads Technologies (Madrid, Spain). Sulfopropyl sepharose, Butyl Sepharose, Octyl Sepharose and Phenyl Sepharose were purchased from GE Healthcare (Uppsala, Sweden). Immobeads-150 Octadecyl, Immobeads-300 Methyl-Estyrene, Lewatit 1600, Polyethyleneimine, Polyallylamine, p-nitrophenyl palmitate, docosahexaenoic acid, Burkholderia cepacia lipase (Amano), Thermomyceslanuginosus lipase (Novozymes), bovine serum albumin and dextran (Leuconostoc mesenteroides) were obtained from Sigma-Aldrich Co. (St. Louis, IL, USA). Methoxypolyethylene glycol amine (NH₂-PEG) was purchased from Rapp Polymere GmbH (Tubingen, Germany). All other reagents were of analytical grade.