Neocaprione

To perform experimental tests, all analytical grade reagents were employed. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Neocaprione, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT), PBS (phosphate-buffered saline, pH 7.4), Dimethylsulfoxide (DMSO), and DCFH-DA (2′,7′-Dichlorofluorescin diacetate) were procured from Sigma Aldrich (St. Louis, MO, USA). Dulbecco Modified Eagle Medium (DMEM), Roswell Park Memorial Institute (RPMI-1640), and fetal bovine serum were purchased from Gibco, Thermo Fisher Scientific (Waltham, MA, USA). Macrophage Raw 264.7 cells were collected from ATCC (Rockville, MD, USA). Lactate dehydrogenase (LDH) release assay kit was obtained from Cayman Chemical (Ann Arbor, MI, USA). Antibodies, including anti-Superoxide dismutase1 (SOD1), anti-Catalase (CAT), anti-Glutathione peroxidase 1 (GPx-1), anti-HO-1, anti-Nrf2, and β-actin and lamin B were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azobis(2-amidinopropane) dihydrochloride (AAPH), phenazine methosulfate (PMS), neocaprione, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-Dichlorofluorescin diacetate (DCFH-DA), phosphate buffered saline (PBS, pH 7.4), and dimethylsulfoxide (DMSO) were purchased from Sigma Aldrich (St. Louis, MO, USA). DMEM (Dulbecco’s modified Eagle’s medium), fetal bovine serum (FBS), penicillin-streptomycin mixture, 0.25% trypsin-EDTA, were purchased from Gibco-BRL Life Technologies (Grand Island, NY, USA). Antibodies purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) included anti-SOD1, anti-HO-1, anti-catalase (CAT), anti-Glutathione peroxidase 1 (GPx-1), anti-Nrf2, whereas anti-phospho-JNK, Anti-p44/42MAPK (ERK1/2), and anti-phospho-p44/42MAPK (ERK1/2), anti-phospho-p38, Anti-p38 were purchased from Cell Signaling Technology (Beverly, MA, USA).