Goat anti rabbit hrp

Manufactured by GE Healthcare

Sourced in Macao

Goat anti-rabbit HRP is a secondary antibody conjugated with horseradish peroxidase (HRP) that binds to rabbit primary antibodies. It is used in various immunoassay techniques, such as Western blotting and enzyme-linked immunosorbent assay (ELISA), to detect and quantify target proteins or analytes.

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Lab products found in correlation

Goat anti mouse hrp, by GE Healthcare (3 mentions) Mouse monoclonal anti gfp, by Roche (2 mentions) Rabbit anti g6pdh, by Merck Group (2 mentions) Goat anti rat hrp, by Abcam (2 mentions) Mouse anti ha, by Fortrea (2 mentions) Rat anti ha, by Roche (2 mentions) 2 nitrophenyl β d galactopyranoside, by Merck Group (1 mentions) Glass bead, by Biospec (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Rabbit anti lysozyme, by Agilent Technologies (1 mentions) Ecl reagent, by GE Healthcare (1 mentions) Pierce halt inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Goat anti mouse 680, by LI COR (1 mentions) Odyssey infrared imager, by LI COR (1 mentions) Donkey anti rabbit 800, by LI COR (1 mentions) Flag tag (flag), by Merck Group (1 mentions)

Close spelling variants of this product

HRP-conjugated goat anti-rabbit IgG (NA934) HRP-conjugated goat anti-rabbit antibody HRP‐conjugated goat anti‐rabbit IgG antibody HRP-conjugated goat anti-mouse or anti-rabbit secondary antibodies (HRP)-coupled goat anti-rabbit IgG Goat-anti-rabbit-HRP conjugate HRP-conjugated goat anti-rabbit HRP-conjugated secondary goat anti-rabbit Goat anti-rabbit IgG-HRP HRP-conjugated goat anti-rabbit IgG

4 protocols using goat anti rabbit hrp

Yeast Strain and Plasmid Characterization

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Yeast (Saccharomyces cerevisiae) strains used in this paper are summarized in S1 Table. Plasmids used in this study are summarized in S2 Table. Gene disruptions and tagging were performed by a standard PCR-based method ([49 (link)]. All chemical reagents were purchased from Fisher Scientific (NJ, USA), except for the following: Nitrogen bases were purchased from US Biological (Swampscott, MA); ProtoGel for Western blots from National Diagnostics (Atlanta, GA); Bacto peptone and Bacto agar from BD Difco (Franklin Lakes, NJ); salmon testes DNA, amino acids, 1-naphthyl phosphate, 2-nitrophenyl β-D-galactopyranoside and protease inhibitors from Sigma Aldrich (St. Louis, MO); glass beads from BioSpec Products (Bartlesville, OK); restriction enzymes and buffers from New England Biolabs (Ipswich, MA).
Antibodies used in this study included mouse monoclonal anti-GFP (Roche Diagnostics), rat anti-HA (Roche Diagnostics), mouse anti-HA (Covance), rabbit anti-G6PDH (Sigma), rabbit anti-Ape1 (a kind gift from Dr. Ohsumi), rabbit anti-Atg8 (a kind gift from Dr. Ohsumi), goat anti-rat HRP (Abcam), goat anti-rabbit HRP and goat anti-mouse HRP (GE Healthcare).

Lipatova Z., Gyurkovska V., Zhao S.F, & Segev N. (2020). Characterization of constitutive ER-phagy of excess membrane proteins. PLoS Genetics, 16(12), e1009255.

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Quantitative Lysozyme Analysis in Organoids

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Organoids were collected in cold medium and sheared by pipetting to generate epithelial fragments that were subsequently washed twice in ice-cold medium to separate cells from debris and lysozyme was secreted into the crypt lumen. Cells were lysed using RIPA buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% SDS, 0.5% Na-Deoxycholate, and 1% NP-40) containing Complete protease and phosphatase inhibitors (Roche). Protein content was quantified using standard Bradford assay. 10 µg of protein was run on a 14% SDS page and eventually transferred to a PVDF membrane and probed with rabbit anti-lysozyme (Dako; 1:10,000 in TBS-T 5% milk) and for normalization mouse anti–α-tubulin (Sigma-Aldrich; 1:3,000). After washes, membranes were incubated with secondary HRP-conjugated antibodies (goat anti–rabbit HRP 1:20,000 and rabbit anti–mouse 1:6,000; GE healthcare) and exposed to x-ray films using ECL reagents (GE Healthcare) and signals were quantified using ImageJ software (National Institutes of Health).

Farin H.F., Karthaus W.R., Kujala P., Rakhshandehroo M., Schwank G., Vries R.G., Kalkhoven E., Nieuwenhuis E.E, & Clevers H. (2014). Paneth cell extrusion and release of antimicrobial products is directly controlled by immune cell–derived IFN-γ. The Journal of Experimental Medicine, 211(7), 1393-1405.

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Western Blot Analysis of Smad Signaling

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Whole cell protein lysates were prepared in RIPA buffer (0.15M NaCl, l% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1% Triton X-100, 1mM phenylmethylsulfonyl fluoride, 0.05M Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Pierce Halt Inhibitor Cocktail, Thermo Scientific). Protein concentrations were estimated by Biorad colorimetric assay (BCA). Bound antibodies were detected with enhanced chemiluminescence (ECL kit, cat #) or by Odyssey Infrared Imager (LI-COR Biosciences). The following primary antibodies were used: Smad4, Smad2, Smad3, phospho-Smad2, phospho-Smad3, Actin, HA (Cell signaling) and FLAG (Sigma-Aldrich). For secondary antibodies, goat anti-rabbit-HRP (GE Healthcare NA934V), goat anti-mouse HRP (GE Healthcare NA931V), goat anti-mouse-680 (Licor 925–32220) donkey anti-rabbit-800 (Licor 926–32213) were used. Dilutions were used according to the recommendation of the respective manufacturers.

Lanauze C.B., Sehgal P., Hayer K., Torres-Diz M., Pippin J.A., Grant S.F, & Thomas-Tikhonenko A. (2021). Colorectal cancer-associated Smad4 R361 hotspot mutations boost Wnt/β-catenin signaling through enhanced Smad4-LEF1 binding. Molecular cancer research : MCR, 19(5), 823-833.

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Yeast Strain and Antibody Protocol

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Yeast (Saccharomyces cerevisiae) strains used in this paper are summarized in Table S1. Plasmids used in this study are summarized in Table S2 Antibodies used in this study included mouse monoclonal anti-GFP (Roche Diagnostics), rat anti-HA (Roche Diagnostics), mouse anti-HA (Covance), rabbit anti-G6PDH
(Sigma), rabbit anti-Ape1 (a kind gift from Dr. Ohsumi), rabbit anti-Atg8 (a kind gift from Dr.
Ohsumi), goat anti-rat HRP (Abcam), goat anti-rabbit HRP and goat anti-mouse HRP (GE Healthcare).

Lipatova Z., Gyurkovska V., Zhao S.F., & Segev N. (2020). Characterization of Constitutive macro-ER-phagy.

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