3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt assay kit

1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-myristoyl-2-stearoyl-sn-glycero-3-phosphocholine (MSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG (2000)), cardiolipin (CL), 1,1′,2,2′-tetraoleoyl cardiolipin [4-(dipyrrometheneboron difluoride)butanoyl] (ammonium salt), Distearoylphosphatidylcholine (DSPC), TopFluor^® CL were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Cholesterol and ammonium sulfate were purchased from JT Baker (Phillipsburg, NJ, USA). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM) and other reagents for cell culture were purchased from Mediatech (Manassas, VA, USA). Daunorubicin was purchased from AvaChem Scientific (San Antonio, TX, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay kit and Phosphate Buffered Saline (PBS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). DAPI (4′,6-diamidino-2-phenylindole) and Bicinchoninic acid protein kit was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Polycarbonate membrane (0.08 μm) was purchased from Whatman plc (Maidstone, Kent, UK). MDA-MB-231 breast cancer cells were obtained from American Type Culture Collection (Manassas, VA, USA).

Iron (III) chloride hexahydrate (FeCl₃·6H₂O), sodium oleate, oleic acid, 1-octadecene, poly (allylamine hydrochloride) (PAH; M_w = 15,000 Da), phosphate buffered saline (PBS), tetramethylammonium hydroxide, N-(3-(dimethylamino)propyl-N′-ethylcarbodiimide) hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (NHS), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay kit and 2ʹ,7ʹ-dichlorohydrofluorescein diacetate (DCFH; ≥ 94%), glucose oxidase (GOx) were purchased from Sigma-Aldrich. 3,3′,5,5′-tetramethylbenzidine (TMB), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), o-phenylenediamine (OPD; 98%), methylene blue (MB), terephthalic acid (TA) and hydrogen peroxide (H₂O₂) assay kit were obtained from Beyotime Biotechnology. Bicinchoninic acid (BCA) protein assay kit, malondialdehyde (MDA) and protein carbonyl assay kit were obtained from Nanjing Jiancheng Institute of Biological Engineering. Live/dead bacteria viability kit was purchased from Thermo Fisher Scientific. All other chemicals were obtained from Adamas-beta and used without further purification. Deionized (DI) water (Millipore Milli-Q grade, 18.2 MΩ) was used in all the experiments.